A new splice variant of the inositol-1,4,5-triphosphate (IP3) receptor.
Cell Signal
; 6(4): 449-55, 1994 May.
Article
em En
| MEDLINE
| ID: mdl-7946968
ABSTRACT
In this study we have identified a new splice variant of the IP3 receptor (IP3R) transcript in a number of mouse cell lines (e.g. mouse T-lymphoma cells, mouse splenic lymphocytes and mouse NIH 3T3 fibroblast cell lines) using the reverse transcriptase-polymerase chain reaction. This variant IP3 receptor (designated as IP3RV-S2, approximately 453 bp) is larger than the non-neuronal form (402 bp) but smaller than the neuronal form (522 bp) of the IP3 receptors. Nucleotide sequencing data indicate that this new isoform (IP3RV-S2) contains a 51 nucleotide insertion within the non-neuronal form of IP3R at the S2 splice site. During mitogenic stimulation by Con A, the ratio between IP3R (non-neuronal form) and IP3RV-S2 (variant isoform) in mouse splenic T-lymphocytes increases approximately 1.5-fold. The change in relative amounts of these two IP3 receptor isoforms during mitogenic-stimulation suggests that T-lymphocytes may have different requirements for the IP3 isoforms in order to control intracellular calcium mobilization. The selective expression of these two IP3R isoforms (IP3RV-S2 and non-neuronal IP3R) may be critically important for the onset of signal transduction and cell activation.
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Base de dados:
MEDLINE
Assunto principal:
DNA Recombinante
/
Canais de Cálcio
/
Inositol 1,4,5-Trifosfato
/
Receptores Citoplasmáticos e Nucleares
Limite:
Animals
Idioma:
En
Revista:
Cell Signal
Ano de publicação:
1994
Tipo de documento:
Article