A comparative study of different experimental protocols for mutagenesis assays with the 9-azaguanine resistance system in cultured Chinese hamster cells.
Mutat Res
; 37(2-3): 293-306, 1976 Nov.
Article
em En
| MEDLINE
| ID: mdl-794707
ABSTRACT
Both spontaneous and EMS-induced mutant frequencies were determined in cultured cells from V79 Chinese hamsters using three different experimental protocols. After optimal expression time was attained, mutation frequencies only remained constant when a protocol was used in whcih the cell density was maintained below critical values both before and during mutant selection. The identification of such a plateau allows, besides more reliable and reproducible estimates of mutation frequency, reduction in the size of experiments for quantitative evaluation of mutagenicity. Determination of mutation frequencies over a wide range of expression times becomes in fact unnecessary.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Genéticas
/
Mutação
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
1976
Tipo de documento:
Article