Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
FEMS Microbiol Lett
; 123(3): 343-7, 1994 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-7988915
ABSTRACT
Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5 alpha MCR was used instead of E. coli DH5 alpha. We used E. coli strains with different mutations in the methyl-specific restriction systems to show that McrBC-deficiency is sufficient to generate this effect. The results imply that C. glutamicum DNA contains methylcytosine in specific sequences recognized by the E. coli McrBC system.
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Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Enzimas de Restrição do DNA
/
Corynebacterium
/
Escherichia coli
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Alemanha