[Studies on the early development of nucleocytoplasmic hybrid embryos between mouse and rabbit by nuclear transplantation].
Shi Yan Sheng Wu Xue Bao
; 26(4): 389-97, 1993 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-8023634
ABSTRACT
Nuclear transplantation has been proved to be a very powerful method in researching the relationship between nucleus and cytoplasm during embryogenesis. Electrofusion is a new means of cell fusion which is being improved in the past nearly ten years. In this study, we produced interorder nucleocytoplasmic hybrid embryos between mouse and rabbit with these two techniques. Two phenomena, i.e. premature chromosome condensation (PCC) and nuclear swelling, were observed after mouse 8-cell stage blastomeres were fused into rabbit enucleated oocytes. After 4.5-days cultured in mouse oviducts, 5.4% reconstituted embryos developed into blastocysts. By karyotype examination, it was known that only mouse chromosomes were presented in the blastomeres, one blastocyst even had a normal mouse karyotype (2 n = 40, XX). As a conclusion, we consider it is possible to obtain normal early development of interspecies nucleocytoplasmic hybrid embryos between mouse and rabbit.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Blastômeros
Limite:
Animals
Idioma:
Zh
Revista:
Shi Yan Sheng Wu Xue Bao
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
China