Secondary in vitro generation of cytolytic T-lymphocytes (CTL's) in the murine sarcoma virus system. Virus-specific CTL induction across the H-2 barrier.
J Natl Cancer Inst
; 61(3): 885-90, 1978 Sep.
Article
em En
| MEDLINE
| ID: mdl-80457
ABSTRACT
Following in vitro stimulation of murine sarcoma virus Moloney isolate (M-MuSV)-immune spleen cells with syngeneic antigenically related Moloney leukemia cells, highly efficient cytotoxic T-lymphocytes (CTL's) were generated. The cytotoxic effect was directed only against H-2-compatible target cells bearing M-MuSV tumor-associated antigens (TAA). However, in a cold target competition assay a weak but detectable capacity to block CTL activity was also obtained when allogeneic Moloney leukemia cells were added. Moreover, when M-MuSV-immune spleen cells from mice inoculated with virus 14 days previously were stimulated by allogeneic Moloney leukemia cells, a strong cytotoxic effect toward syngeneic and allogeneic tumor cells bearing M-MuSV TAA was elicited.
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Base de dados:
MEDLINE
Assunto principal:
Sarcoma Experimental
/
Linfócitos T
/
Citotoxicidade Imunológica
/
Antígenos de Neoplasias
Limite:
Animals
Idioma:
En
Revista:
J Natl Cancer Inst
Ano de publicação:
1978
Tipo de documento:
Article