Autocrine stimulation of motility in SBC-5 human lung carcinoma cells by a two-kringle variant of HGF.
Cancer Lett
; 83(1-2): 235-43, 1994 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-8062221
ABSTRACT
Hepatocyte growth factor (HGF), a ligand for the c-met protooncogene product, is a pleiotropic cytokine which elicits mitogenic, motogenic and morphogenic activities. Among various human lung carcinoma cells, we found that SBC-5 small cell lung carcinoma cells simultaneously expressed the c-Met/HGF receptor and a smaller variant-type of HGF composed of N-terminal two-kringle domains, without expressing authentic heterodimeric HGF. The addition of anti-HGF antibodies to cultures of SBC-5 cells specifically inhibited spreading and motility of the cells without affecting growth, and the conditioned medium of SBC-5 cells also induced scattering of other lineage lung carcinoma. Thus, simultaneous expression of the unique smaller variant HGF and its receptor, c-Met, in SBC-5 cells suggests the involvement of a smaller variant HGF in the development or progression of the lung carcinoma cells, through an autocrine mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma
/
Fator de Crescimento de Hepatócito
/
Neoplasias Pulmonares
Limite:
Humans
Idioma:
En
Revista:
Cancer Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Japão