The OLFR1 gene encoding the HGMP07E putative olfactory receptor maps to the 17p13-->p12 region of the human genome and reveals an MspI restriction fragment length polymorphism.
Cytogenet Cell Genet
; 63(3): 200-4, 1993.
Article
em En
| MEDLINE
| ID: mdl-8097991
ABSTRACT
Olfactory receptors are believed to be encoded by an extremely large subfamily of G protein-coupled receptors. A human gene (OLFR1) encoding a member of this family was cloned from a genomic library by cross-hybridization with a gene fragment obtained by the polymerase chain reaction. The nucleotide sequence of a 3.4-kb EcoRI fragment was determined, and the protein sequence was deduced from the single open reading frame. The gene was assigned by in situ hybridization of metaphase chromosomes to the 17p13-->p12 region of the human genome, in proximity to the tumor-suppressor gene encoding p53. When used as a probe on Southern blots under moderately stringent conditions, it hybridizes to at least three closely related genes. A restriction fragment length polymorphism was detected after MspI digestion. Mendelian segregation of the gene was assessed in three CEPH families, and linkage analysis confirmed the localization of the locus.
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Base de dados:
MEDLINE
Assunto principal:
Células Receptoras Sensoriais
/
Olfato
/
Cromossomos Humanos Par 17
/
Receptores Odorantes
Limite:
Humans
Idioma:
En
Revista:
Cytogenet Cell Genet
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Bélgica