A novel approach to the laboratory diagnosis of Chlamydia trachomatis infections using monoclonal anti-idiotypic antibodies.
J Immunol Methods
; 163(1): 123-31, 1993 Jul 06.
Article
em En
| MEDLINE
| ID: mdl-8101547
ABSTRACT
We have developed a novel enzyme immunoassay (EIA) for the specific detection of Chlamydia trachomatis utilizing a monoclonal anti-idiotypic antibody to an antibody directed against a chlamydia specific epitope on 60 kDa heat-shock protein (HSP60). The basis of the assay is the inhibition of the binding of idiotype to anti-idiotype by antigen present in test samples. Two configurations of the assay were developed a blocking EIA and a competition EIA. Greater sensitivity was observed using the competition EIA, with the assay detecting purified recombinant HSP60 and purified chlamydia in a concentration-dependent manner from 0.01 to 10 micrograms protein and from 0.5 to 12 micrograms total protein, respectively. The assay is highly specific and offers several potential advantages over currently available EIAs for the detection of this pathogen.
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Base de dados:
MEDLINE
Assunto principal:
Infecções por Chlamydia
/
Chlamydia trachomatis
/
Anticorpos Anti-Idiotípicos
/
Técnicas Imunoenzimáticas
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Canadá