Characterization of the oligosaccharide moiety of VIP receptor from the human pancreatic cell line BxPC-3.
Peptides
; 14(6): 1331-8, 1993.
Article
em En
| MEDLINE
| ID: mdl-8134315
ABSTRACT
The human pancreatic cell line BxPC-3 displays two classes of binding sites with high and low affinity for VIP. The order of potency of VIP-related peptides in inhibiting either [125I]VIP or [125I]N-AcPACAP27 binding and in stimulating cAMP production was typical of the human VIP receptor. By combining affinity labeling with glycosidase treatments, we have characterized the VIP receptor as a M(r) = 68,200 glycoprotein, consisting of a M(r) = 39,300 polypeptide core with at least three N-linked oligosaccharide chains. In addition, our results revealed the presence of a low amount of sialic acid residues in the carbohydrate moiety of receptor.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Glicoproteínas
/
Adenocarcinoma
/
Receptores de Peptídeo Intestinal Vasoativo
Limite:
Humans
Idioma:
En
Revista:
Peptides
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França