Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation.
Plant Mol Biol
; 23(4): 839-46, 1993 Nov.
Article
em En
| MEDLINE
| ID: mdl-8251636
ABSTRACT
A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.
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Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Verduras
/
Calmodulina
/
Regulação da Expressão Gênica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Plant Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
França