Effect of aggregation on the kinetics of autoxidation of the polyene antibiotic amphotericin B.
J Pharm Sci
; 82(2): 162-6, 1993 Feb.
Article
em En
| MEDLINE
| ID: mdl-8383201
ABSTRACT
We have previously studied the autoxidation of the polyene antibiotic amphotericin B (AB). In this paper we describe the dependence of the kinetics of autoxidation on the aggregation state of the antibiotic. Autoxidation, which is involved in drug inactivation and has been suggested to play a role in the mechanism of drug action, was assessed through the reaction of formed radicals with the spin label Tempol (2,2,6,6-tetramethyl-4-hydroxy-N-oxylpiperidine) by following the loss of the electron spin resonance signal, as previously described, and by oxygen consumption. Two types of AB (I and II) were used, the former being obtained by further purification of the latter. The kinetics of autoxidation were compared for aggregates formed by the antibiotic. Differences in aggregation state for both type I and type II AB were observed between monomeric, borax-complexed, and preparations in water containing variable proportions of dimethyl sulfoxide (DMSO) by optical absorption and circular dichroism (CD) spectra. On the other hand, although the suspensions of type I and type II AB in water-10% DMSO did not differ in their optical properties, they could be distinguished by quasielastic light scattering experiments, type II yielding smaller aggregates. It is proposed that the lack of difference in optical and CD spectra are due to the similarity of the microenvironments in both aggregates. In contrast, the borax complex of both type I and type II AB yielded similar optical and CD spectra and quasielastic light scattering behavior, indicating that complexation led to similar aggregates.(ABSTRACT TRUNCATED AT 250 WORDS)
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Anfotericina B
Idioma:
En
Revista:
J Pharm Sci
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Brasil