Lipopolysaccharide promoted opening of the porin channel.
FEBS Lett
; 320(3): 251-5, 1993 Apr 12.
Article
em En
| MEDLINE
| ID: mdl-8385028
ABSTRACT
We show here that the imipenem (a carbapenem, beta-lactam antibiotic)-permeable porin channels (protein D2 or OprD2) of Pseudomonas aeruginosa were closed mostly in the lipopolysaccharide (LPS)-free membrane and were openable by adding LPS to the membrane as assayed by ion conductivity measurements using planar lipid bilayers. Open and closed states of the OprD2 channels exhibited conductivities of about 400 and 30 pS, respectively, in 1 M NaCl. The OprD2 channel in the LPS-containing membrane showed very rapid opening and closing events in a second order and the duration of closure became longer at low membrane potentials.
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Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Proteínas da Membrana Bacteriana Externa
/
Lipopolissacarídeos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão