Variable and constant regions in the C-terminus of vinculin and metavinculin. Cloning and expression of fragments in E. coli.
FEBS Lett
; 317(3): 189-94, 1993 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-8425604
ABSTRACT
Metavinculin differs from vinculin in having an additional insert of 68 to 79 amino acids in length in the C-terminal half of the molecule. Cross-species comparison of metavinculin sequences from pig, man, chicken and frog reveals a division of the insert into two parts the first variable and the second highly conserved. The longest insert, 79 amino acids, was found in Xenopus laevis. Three different C-terminal constructs of vinculin and metavinculin over-expressed in E. coli could be purified by column chromatography. Two-dimensional gel electrophoresis and peptide analysis revealed pI values between 8.35 and 10.25 for the recombinant proteins. Biochemical and structural features of the metavinculin-specific sequence and the conserved vinculin/metavinculin carboxy-terminus are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Vinculina
/
Proteínas Aviárias
/
Proteínas Musculares
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1993
Tipo de documento:
Article