Identification of a Drosophila homologue of alpha-catenin and its association with the armadillo protein.
J Cell Biol
; 121(5): 1133-40, 1993 Jun.
Article
em En
| MEDLINE
| ID: mdl-8501118
ABSTRACT
The cadherin cell adhesion system plays a central role in cell-cell adhesion in vertebrates, but its homologues are not identified in the invertebrate. alpha-Catenins are a group of proteins associated with cadherins, and this association is crucial for the cadherins' function. Here, we report the cloning of a Drosophila alpha-catenin gene by low stringent hybridization with a mouse alpha E-catenin probe. Isolated cDNAs encoded a 110-kD protein with 60% identity to mouse alpha E-catenin, and this protein was termed D alpha-catenin. The gene of this protein was located at the chromosome band 80B. Immunostaining analysis using a mAb to D alpha-catenin revealed that it was localized to cell-cell contact sites, expressed throughout development and present in a wide variety of tissues. When this protein was immunoprecipitated from detergent extracts of Drosophila embryos or cell lines, several proteins co-precipitated. These included the armadillo product which was known to be a Drosophila homologue of beta-catenin, another cadherin-associated protein in vertebrates, and a 150-kD glycoprotein. These results strongly suggest that Drosophila has a cell adhesion machinery homologous to the vertebrate cadherin-catenin system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Moléculas de Adesão Celular
/
Transativadores
/
Genes de Insetos
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Proteínas do Citoesqueleto
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Proteínas de Drosophila
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Drosophila melanogaster
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Japão