Functional characterisation of the Drosophila 5-HTdro1 and 5-HTdro2B serotonin receptors in insect cells: activation of a G(alpha) s-like protein by 5-HTdro1 but lack of coupling to inhibitory G-proteins by 5-HTdro2B.
FEBS Lett
; 381(3): 233-6, 1996 Mar 04.
Article
em En
| MEDLINE
| ID: mdl-8601462
ABSTRACT
Insect cells are routinely used for the production of receptor proteins. Expression of the Drosophila 5-HTdro1 serotonin receptor resulted in positive coupling of the receptor to adenylyl cyclase via the G(alpha)s G-protein subtype. The Drosophila 5-HTdro2B receptor stimulated the metabolism of inositol phospholipid via a pertussis toxin-insensitive G-protein, but exhibited no detectable inhibition of adenylyl cyclase. Immunoblot analysis of the endogenous G-proteins revealed that Sf9 cells lack the G-protein subtypes G(alpha i 1-3) and G(alpha)o, but express the subtype G(alpha)s and G(alpha)q.
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Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Receptores de Serotonina
/
Proteínas de Ligação ao GTP
/
Drosophila
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Reino Unido