Rad23 is required for transcription-coupled repair and efficient overrall repair in Saccharomyces cerevisiae.
Mol Cell Biol
; 16(5): 2361-8, 1996 May.
Article
em En
| MEDLINE
| ID: mdl-8628303
ABSTRACT
The repair of UV-induced photoproducts (cyclobutane pyrimidine dimers) in a well-characterized minichromosome, genomic DNA, and a transcribed genomic gene (RPB2) of a rad23delta mutant of Saccharomyces care was examined. Isogenic wild-type cells show a strong bias for the repair of the transcribed strands in both the plasmid and genomic genes and efficient overall repair of both DNAs (>80% of the dimers were removed in 6 h). However, the rad23delta mutant shows (i) no strand bias for repair in these genes and decreased repair of both strands, (ii) partial repair of genomic DNA (approximately 45% in 6 h), and (iii) very poor repair of the plasmid overall approximately 15% in 6 h). These features, coupled with the decreased UV survival of rad23delta cells, indicate that Rad23 is required for both transcription-coupled repair and efficient overall repair in S. cerevisiae.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Transcrição Gênica
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Raios Ultravioleta
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Proteínas Fúngicas
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Ligação a DNA
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Reparo do DNA
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos