Coexpression with potassium channel subunits used to clone the Y2 receptor for neuropeptide Y.
Mol Pharmacol
; 49(3): 387-90, 1996 Mar.
Article
em En
| MEDLINE
| ID: mdl-8643076
ABSTRACT
Xenopus oocytes were injected with RNAs for the two inward-rectifier potassium channel subunits Kir3.1 (GIRK1) and Kir3.4 (rcKATP or CIR) in addition to RNA from the neuroblastoma cell line KAN-TS. Potassium currents were evoked by neuropeptide Y in oocytes injected with polyadenylated RNA or with cRNA from pools of a neuroblastoma (KAN-TS) cDNA library, and progressive subdivision of responding pools yielded a single cDNA. The encoded protein contains 381 amino acids, has the seven hydrophobic domains characteristic of G protein-coupled receptors, and is 31% identical to the Y1 receptor potassium currents were induced by neuropeptide Y (EC50=60pm) and Y2-selective analogues. Coexpression with potassium channel subunits will be a generally useful method for the cloning of G protein-coupled receptors.
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Base de dados:
MEDLINE
Assunto principal:
Neuropeptídeo Y
/
Canais de Potássio
/
Receptores de Neuropeptídeo Y
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mol Pharmacol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Itália