Expression of biologically active isoforms of the tumor angiogenesis factor VEGF in Escherichia coli.
Biochem Biophys Res Commun
; 222(2): 249-55, 1996 May 15.
Article
em En
| MEDLINE
| ID: mdl-8670191
ABSTRACT
Vascular endothelial growth factor (VEGF) was identified as an endothelial cell specific mitogen that induces angiogenesis and vascular permeability in vivo. VEGF is a homodimeric protein which contains three intramolecular and two intermolecular disulfide bridges. Here, we report on an efficient procedure for recombinant production of VEGF isoforms VEGF121 and VEGF165 in Escherichia coli. The proteins were solubilized from inclusion bodies, refolded, and purified by chromatographic methods. The final protein products were almost completely in the dimeric conformation, bound to VEGF receptor FLT1 with a Kd of 30 pM, stimulated proliferation of human umbilical vein endothelial cells half-maximally at a concentration of 30 pM, and induced in vivo neovascularization and vascular permeability on the chicken chorioallantoic membrane.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Endotélio Vascular
/
Fatores de Crescimento Endotelial
/
Linfocinas
/
Receptores de Fatores de Crescimento
/
Receptores Proteína Tirosina Quinases
/
Neovascularização Fisiológica
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1996
Tipo de documento:
Article