[Vaccination with genetically modified IL-2 secreting cells in a rat model of colonic carcinoma]. / Vaccination par des cellules génétiquement modifiées sécrétant de l'IL-2 dans un modèle de carcinome colique de rat.
Bull Cancer
; 83(3): 218-26, 1996 Mar.
Article
em Fr
| MEDLINE
| ID: mdl-8695924
ABSTRACT
Genetically engineered tumor cells secreting immunostimulatory molecules could facilitate the obtention of a vaccination against tumor antigens. To test this approach, we transfected genes encoding for rat and mouse IL-2 into PROb cells. These cells originate from a dimethylhydrazine induced colon carcinoma of BD IX rats. We observed an inhibition of the in vivo tumor growth directly proportional to the IL-2 secretion. An immunohistochemical analysis revealed that the tumors were infiltrated by leucocytes expressing the IL-2 receptor, suggesting their activation within the tumor. A strong delay of tumor growth was observed in rats challenged with PROb cells after a previous rejection of IL-2 secreting cells. Yet two rats out of six were completely protected. This protection is specific since rejection of PROb-IL-2 does not confer protection towards the syngeneic glioma A15A5. In addition, we could show by depletion experiments that NK/LAK, CD8, and CD4 lymphocytes were involved in the rejection of cells secreting large amounts of IL-2. Macrophages appear to be involved in the rejection process too, but also in the induction of an immune memory. Vaccination experiments using irradiated PROb IL-2 cells were performed. Only a partial protection towards a challenge with parental PROb cells could be obtained, also depending on the amount of secreted IL-2 the best protection being obtained after vaccination with cells synthesizing a small amount of IL-2. However, this protection was not superior to that obtained by coinjection of irradiated PROb cells and BCG.
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
/
Interleucina-2
/
Neoplasias do Colo
/
Imunoterapia
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Fr
Revista:
Bull Cancer
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
França