Preparation and surface characterization of functional group-grafted and heparin-immobilized polyurethanes by plasma glow discharge.
Biomaterials
; 17(8): 841-7, 1996 Apr.
Article
em En
| MEDLINE
| ID: mdl-8730969
ABSTRACT
Functional group-grafted polyurethanes were prepared by oxygen plasma discharge treatment, followed by graft polymerization of 1-acryloylbenzotriazole (AB) and a subsequent substitution reaction of AB with sodium hydroxide and ethylene diamine. The primary amine or carboxylic acid groups grafted on the surfaces were coupled with heparin using water-soluble carbodiimide. The modified surfaces were characterized by measuring the water contact angle, electron spectroscopy for chemical analysis and attenuated total reflection Fourier-transform infrared spectroscopy. The amount of heparin covalently immobilized on the primary amine- and carboxylic acid group-grafted polyurethanes were 2.0 and 1.4 micrograms cm-2, respectively, as determined by the toluidine blue method. The water contact angle of the polyurethanes was decreased by AB grafting, and further decreased by the introduction of functional groups such as carboxylic acid and primary amine and immobilization of heparin, showing increased hydrophilicity of the modified surfaces. Heparin was almost not released from the immobilized surfaces in the physiological solution for 100 h, indicating good stability of immobilized heparin.
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Base de dados:
MEDLINE
Assunto principal:
Poliuretanos
/
Heparina
/
Reagentes de Ligações Cruzadas
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
1996
Tipo de documento:
Article