Leishmania major: targeting IL-4 in successful immunomodulation of murine infection.
Exp Parasitol
; 84(2): 214-22, 1996 Nov.
Article
em En
| MEDLINE
| ID: mdl-8932771
ABSTRACT
Protection against Leishmania major infection among inbred strains of mice is dependent upon successful expansion and activation of type 1 CD4+ effector (Th1) cells, a process that is aberrant in highly susceptible BALB strains. We sought to establish whether vaccination strategies using whole parasite lysates or a characterized immunodominant antigen, the Leishmania homolog of mammalian receptor for activated protein kinase C (LACK), would be capable of protecting subsequently infected BALB mice if given within a cytokine milieu capable of biasing the immune response toward Th1 cells. When given with neutralizing antibody to IL-4, but not when given alone, subcutaneously administered soluble Leishmania antigens mediated substantial protection to BALB/c mice against subsequent infection with parasites as assessed by size of the local lesion, enhanced Th1-type immune responses, and decreased parasite burdens. Similarly, when given with recombinant IL-12, LACK conferred substantial protection to cohorts of BALB.B, BALB/c, and BALB.K mice that was associated with reduction in serum IgE levels, consistent with effects on IL-4 production. Thus altering the cytokine milieu during administration of vaccine antigens by neutralizing IL-4 induced powerful Th1 recall responses during infection that were capable of mediating substantial levels of protection.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Protozoários
/
Interleucina-4
/
Leishmaniose Cutânea
/
Leishmania major
/
Antígenos de Protozoários
Limite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Estados Unidos