Measurement of in vivo DNA binding by sequence-specific transcription factors using UV cross-linking.
Methods
; 11(2): 215-24, 1997 Feb.
Article
em En
| MEDLINE
| ID: mdl-8993034
ABSTRACT
This paper describes an in vivo UV cross-linking protocol that is sensitive enough to detect DNA binding by sequence-specific transcription factors in Drosophila embryos and tissue culture cells. The strength of this approach is that it provides a quantitative measure of DNA binding in vivo with unambiguous identification of the factor involved in the binding. This assay often detects DNA binding properties of proteins that were not predicted from previous experiments, and it can be used to directly test diverse models of gene regulation in the context of a living organism.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Methods
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos