Fructan synthesis in transgenic tobacco and chicory plants expressing barley sucrose: fructan 6-fructosyltransferase.
FEBS Lett
; 400(3): 355-8, 1997 Jan 06.
Article
em En
| MEDLINE
| ID: mdl-9009230
ABSTRACT
We have recently cloned a cDNA encoding sucrosefructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the beta-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652-11656]. Here we report functional expression of 6-SFT from barley in transgenic tobacco and chicory. Transformants of tobacco, a plant naturally unable to form fructans, synthesized the trisaccharide kestose and a series of unbranched fructans of the phlein type (beta-2,6 linkages). Transformants of chicory, a plant naturally producing only unbranched fructans of the inulin type (beta-2,1 linkages), synthesized in addition branched fructans of the graminan type, particularly the tetrasaccharide bifurcose which is also a main fructan in barley leaves.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Tóxicas
/
Nicotiana
/
Hordeum
/
Plantas Geneticamente Modificadas
/
Frutanos
/
Hexosiltransferases
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Suíça