Tn5 tagging of the phenol-degrading gene on the chromosome of Pseudomonas putida.
Mol Cells
; 7(1): 40-4, 1997 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-9085263
ABSTRACT
Transposon mutagenesis was performed by the method of conjugational transfer in order to identify and characterize genes encoding enzymes involved in the pathway of phenol utilization as a carbon source. Escherichia coli, which carries the Tn5-132, Was mated with Pseudomonas putida SM25 as a host. We selected a mutant that could not utilize phenol as a carbon source. Chromosomal integration of the transposon was confirmed by Southern analysis, successfully tagging the gene related to a phenol-utilizing pathway. By cell-free enzyme and genetic complementation assays, the inactivated enzyme through the mutation of the corresponding gene was identified as the catB gene, which encodes a cis,cis-muconate lactonizing enzyme.
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Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Cromossomos Bacterianos
/
Pseudomonas putida
/
Liases Intramoleculares
/
Genes Bacterianos
Idioma:
En
Revista:
Mol Cells
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1997
Tipo de documento:
Article