Use of recombinant vaccinia to restimulate antigen specific human peripheral blood cytotoxic T lymphocytes.
J Virol Methods
; 65(1): 105-9, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9128867
ABSTRACT
A simple method is described for using recombinant vaccinia virus for restimulating human peripheral blood mononuclear cells (PBMC) to generate antigen specific CD8 + alpha beta cytotoxic T cell (CTL) effector populations. Effector PBMC were restimulated in vitro with a subpopulation of PBMC, which had been infected with recombinant vaccinia at very low multiplicities of infection in the absence of serum. This protocol avoided the vaccinia mediated overt cytopathic effects on the effector PBMC and generated bulk CTL cultures, which could be used for the identification of epitopes recognised by antigen specific CTL.
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Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
/
DNA Recombinante
/
Linfócitos T Citotóxicos
/
Epitopos
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Austrália