Isolation of a Dictyostelium discoideum 14-3-3 homologue.
Biochim Biophys Acta
; 1357(2): 243-8, 1997 Jun 27.
Article
em En
| MEDLINE
| ID: mdl-9223628
ABSTRACT
A 1.0 kb cDNA clone (Dd14-3-3) encoding a 14-3-3 homologue was isolated from a Dictyostelium discoideum cDNA library. The putative Dd14-3-3 protein has highest sequence identity to a barley 14-3-3 isoform (74%). Southern blot analysis suggests that only one 14-3-3 gene is present in the Dictyostelium genome. Highest Dd14-3-3 expression is observed in vegetatively growing cells, and expression decreases during multicellular development. In contrast, Dd14-3-3 protein levels detected immunochemically remained constant during Dictyostelium development. Expression of the Dd14-3-3 cDNA in Saccharomyces cerevisiae complemented the lethal disruption of the two yeast genes encoding 14-3-3 proteins (BMH1 and BMH2). This shows that Dd14-3-3 can fulfil the same function(s) as the yeast 14-3-3 proteins.
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Base de dados:
MEDLINE
Assunto principal:
Tirosina 3-Mono-Oxigenase
/
Proteínas
/
Dictyostelium
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Holanda