Estrogen receptor mRNA in mineralized tissues of rainbow trout: calcium mobilization by estrogen.
FEBS Lett
; 411(1): 145-8, 1997 Jul 07.
Article
em En
| MEDLINE
| ID: mdl-9247160
ABSTRACT
RT-PCR was undertaken on total RNA extracts from bone and scales of the rainbow trout, Oncorhynchus mykiss. The rainbow trout estrogen receptor (ER)-specific primers used amplified a single product of expected size from each tissue which, using Southern blotting, strongly hybridized with a 32P-labelled rtER probe under stringent conditions. These data provide the first in vivo evidence of ER mRNA in bone and scale tissues of rainbow trout and suggest that the effects of estrogen observed in this study (increased bone mineral and decreased scale mineral contents, respectively) may be mediated directly through ER.
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Base de dados:
MEDLINE
Assunto principal:
Osso e Ossos
/
Receptores de Estrogênio
/
Cálcio
/
Oncorhynchus mykiss
/
Estradiol
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Reino Unido