Biogenic 4-hydroxy-2-nonenal activates transcription factor AP-1 but not NF-kappa B in cells of the macrophage lineage.
Biofactors
; 6(2): 173-9, 1997.
Article
em En
| MEDLINE
| ID: mdl-9259999
ABSTRACT
A large spectrum of pro-oxidant agents, including molecules with lipoperoxidative effect, can modulate gene expression through modification of the DNA binding activity of the transcription factors activator protein 1 (AP-1) and nuclear factor kappa B (NF-kappa B). In this study the effect on these redox-sensitive factors by 4-hydroxy-2-nonenal (HNE), a major aldehydic product of lipid peroxidation, was examined in two cell lines of the macrophage type. Incubation in the presence of microM concentrations of the aldehyde led to a rapid increase of AP-1 binding with a transient maximum 30 min from HNE addition to the culture medium in both cell lines. On the contrary, HNE did not stimulate nuclear translocation of NF-kappa B. The diverging effect of HNE on the two transcription factors is likely related to the demonstrated differential activation pathway of AP-1 and NF-kappa B in macrophages. The HNE-induced activation of AP-1 suggests the aldehyde's involvement in the regulatory mechanisms of cell proliferation and differentiation.
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Base de dados:
MEDLINE
Assunto principal:
NF-kappa B
/
Fator de Transcrição AP-1
/
Aldeídos
/
Macrófagos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biofactors
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Itália