Novel dimeric Nur77 signaling mechanism in endocrine and lymphoid cells.
Mol Cell Biol
; 17(10): 5946-51, 1997 Oct.
Article
em En
| MEDLINE
| ID: mdl-9315652
ABSTRACT
Within the nuclear receptor family, Nur77 (also known as NGFI-B) distinguishes itself by its ability to bind a target sequence (the NBRE) as a monomer and by its role in T-cell receptor (TCR)-induced apoptosis in T cells. We now report on a novel mechanism of Nur77 action that is mediated by homodimers. These dimers bind a Nur77 response element (NurRE), which has been identified as a target of CRH-induced Nur77 in the pro-opiomelanocortin (POMC) gene promoter. Both halves of the palindromic NurRE are required for responsiveness to physiological signals, like CRH in pituitary-derived AtT-20 cells. Similarly, in T-cell hybridomas, TCR activation induced NurRE but not NBRE reporters. The in vivo signaling function of Nur77 thus appears to be mediated by dimers acting on a palindromic response element of unusual spacing between its half-sites. This mechanism may represent the biologically relevant paradigm of action for this subfamily of orphan nuclear receptors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adeno-Hipófise
/
Fatores de Transcrição
/
Pró-Opiomelanocortina
/
Linfócitos T
/
Transdução de Sinais
/
Receptores Citoplasmáticos e Nucleares
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Canadá