DNA-chlorpheniramine interaction studied by spectroscopic techniques.

ABSTRACT

It was previously studied that the antihistaminic chlorpheniramine elicits a biphasic response on cell growth and regulates polyamine metabolism, as described for polyamines. In part, polyamine effects on macromolecular synthesis and cell growth are attributed to nucleic acidpolyamine interactions. In this work, we have tested the hypothesis of a DNAchlorpheniramine interaction, using fluorometry, FTIR and Raman spectroscopic techniques. The results indicate that DNAchlorpheniramine interaction occurs inducing conformational changes in the macromolecule by affecting both phosphodiester bonds and bases. Results open new perspectives for characterization of action mechanisms of natural or synthetic diamines with pharmacological or physiological importance.