Developmental regulation of neuroligand-induced responses in cultured oligodendroglia.
Neuroreport
; 9(6): 973-80, 1998 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-9601652
ABSTRACT
Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+ concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and metenkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Córtex Cerebral
/
Oligodendroglia
/
Neurotransmissores
Limite:
Animals
Idioma:
En
Revista:
Neuroreport
Assunto da revista:
NEUROLOGIA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Bélgica