Poly-(3-hydroxybutyrate) production from whey by high-density cultivation of recombinant Escherichia coli.
Appl Microbiol Biotechnol
; 50(1): 30-3, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9720197
ABSTRACT
Recombinant Escherichia coli strain GCSC 6576, harboring a high-copy-number plasmid containing the Ralstonia eutropha genes for polyhydroxyalkanoate (PHA) synthesis and the E. coli ftsZ gene, was employed to produce poly-(3-hydroxybutyrate) (PHB) from whey, pH-stat fed-batch fermentation, using whey powder as the nutrient feed, produced cellular dry weight and PHB concentrations of 109 g l-1 and 50 g l-1 respectively in 47 h. When concentrated whey solution containing 210 g l-1 lactose was used as the nutrient feed, cellular dry weight and PHB concentrations of 87 g l-1 and 69 g l-1 respectively could be obtained in 49 h by pH-stat fed-batch culture. The PHB content was as high as 80% of the cellular dry weight. These results suggest that cost-effective production of PHB is possible by fed-batch culture of recombinant E. coli using concentrated whey solution as a substrate.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Leite
/
Escherichia coli
/
Hidroxibutiratos
Limite:
Animals
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
1998
Tipo de documento:
Article