Extracellular H+ stimulates the expression of c-fos/c-jun mRNA through Ca2+/calmodulin in PC12 cells.
Cell Signal
; 10(7): 499-503, 1998 Jul.
Article
em En
| MEDLINE
| ID: mdl-9754718
ABSTRACT
Evidence has accumulated that an increase in extracellular protons stimulates the transmembrane mechanism to induce various intracellular responses, such as the expression of c-fos and c-jun. In the present study, we aimed to obtain evidence that an increase in extracellular protons induces expression of c-fos/c-jun mRNA in PC12 pheochromocytoma cells of rats. We found that the c-fos/c-jun mRNA expression increased when extracellular pH was decreased gradually from 7.40 to 7.20 and that there was a significant correlation between extracellular pH values and the expression of c-fos/c-jun mRNA. To determine whether the Ca2+/calmodulin system subserves the H+-induced expression of c-fos/c-jun, Ca2+/calmodulin inhibitor trifluoperazine was added to PC12 cells. We found that trifluoperazine inhibited the expression of the H+-induced c-fos/c-jun mRNA by 30-35%. In contrast, trifluoperazine did not inhibit the expression of phorbol-induced c-fos/c-jun mRNA. These results indicate that an increase in extracellular protons induces the expression of c-fos/c-jun mRNA, and this expression is mediated partly by the Ca2+/calmodulin system.
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Base de dados:
MEDLINE
Assunto principal:
Calmodulina
/
Regulação da Expressão Gênica
/
Cálcio
/
Proteínas Proto-Oncogênicas c-jun
/
Proteínas Proto-Oncogênicas c-fos
/
Hidrogênio
Limite:
Animals
Idioma:
En
Revista:
Cell Signal
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Japão