Postmortem Interval Estimation With Corneal Endothelial Cell Density.

OBJECTIVES: In the neonatal period, healthy people have the highest corneal endothelial cell density (CECD) (5000-7000 cells/mm2). Corneal endothelial cell density declines with age in adults (2500-3000 cells/mm2) and continues to decline in the postmortem period. We measured CECD in cases with different postmortem interval (PMI) and investigated its association with PMI. METHODS: A total of 555 corneas harvested from 285 cases with a known time of death were examined using a specular microscope. RESULTS: Postmortem corneal removal time ranged between 10 and 1395 minutes. The CECD averages were 2653 for right cornea and 2678 cells/mm2 for left cornea. An inverse but nonlinear relationship was found between age and CECD. In both men and women, there was an inverse and weak correlation between age and CECD (ρ = -0.282; P < 0.001; ρ = -0.264; P < 0.001, respectively). There was no significant relationship between postmortem corneal removal time and CECD (ρ = 0.049; P = 0.421; ρ = 0.011; P = 0.855 for right and left corneas, respectively). The highest decline in time dependent CECD was detected between the 480th and 540th minutes at -7.2%. CONCLUSIONS: We found no significant decrease in CECD numbers according to PMI or cause of death. Experimental studies on cases with known and standardized antemortem CECD values will provide essential results in estimating PMI.

Non-suicidal self-injurious behavior in forensic child and adolescent populations. Clinical features and relationship with depression.

OBJECTIVE: To investigate risk factors for non-suicidal self-injury (NSSI), and present the relationship between NSSI and depression in children and adolescents who appeared for forensic examination. METHODS: This study consisted of 295 children and adolescents who were brought for judicial examination in the TR Ministry of Justice Forensic Science Department, Council of Forensic Medicine, Ankara, Turkey between May and October 2013. Sociodemographic factors, alcohol and substance abuse, and history of sexual abuse and suicide attempts were assessed using a semi-structured questionnaire. During forensic medical examinations, NSSI was evaluated. Depression was assessed using the Beck Depression Inventory. RESULTS: The frequency of NSSI was 20.2% among boys, and 30.6% among girls. Statistical differences were found between subjects with and without NSSI in terms of number of children in their families, whether or not their parents were divorced, whether they held part-time jobs, or had a history of sexual abuse, substance abuse, or suicide attempts, and the number of criminal involvements. Those with NSSI had higher depressive scores than others (p<0.001). CONCLUSION: Children and adolescents with NSSI have wide-ranging problems in their lives. In a forensic adolescent population, depressive symptoms are more common in individuals with NSSI behaviors, and the specific characteristics of these behaviors need further investigation.

Evaluation of social and demographic characteristics of incest cases in a university hospital in Turkey.

BACKGROUND: Incest is defined as any sexual activity between close blood relatives including step relatives and family members who are forbidden by law to marry. It is a problem that can be seen in all the social classes in developed and undeveloped societies. The World Health Organization classifies this problem as a silent health emergency. Father-daughter incest is reported to be the most common incest type followed by the other types like brother-sister, sister-sister and mother-son incest. MATERIAL AND METHODS: Subjects for this study were recruited from a sample of incest cases referred to Forensic Medicine Department of Gaziosmanpasa University Medical Faculty Hospital between 2008 and 2012. Data involved social and demographic characteristics and clinical features of victims, perpetrators and the families. The ethical committee of the faculty of medicine approved the study. RESULTS: The study sample consisted of 43 incest cases (36 females and 7 males) with an age rage 4-40 years. Two third of the victims were under 18 years old. All perpetrators were males. Father - daughter incest (34.9%) was found to be most common incest type followed by brother - sister incest (14%). 75% of the perpetrators were family members and relatives with consanguinity while 25% of them were not consanguineous but faithful and intimate relatives to victims. CONCLUSIONS: Increasing awareness about incest and its damaging effects is so important and clinicians should keep in mind sexual abuse or incest when examining the risky population. Multidisciplinary approach is necessary for determining short term or long term results and preventing the negative consequences of incest.

Comprehensive examination of etiological factors and clinical manifestations of maxillofacial traumas in forensic cases: A five-year retrospective study.

BACKGROUND: Maxillofacial injuries, due to their diverse etiological causes, are often considered a component of multi-trauma and constitute a significant portion of trauma. This study aims to elucidate the incidence of maxillofacial traumas, particularly among military personnel, various clinical courses, and characteristics, thereby contributing to the literature. METHODS: Forensic reports, primarily related to military personnel and organized between 2011 and 2016 at the Forensic Medicine Department of Gülhane Medical Faculty, Health Sciences University, were retrospectively examined. The study involved a detailed analysis of cases with maxillofacial injuries resulting from trauma, focusing on aspects such as age, gender, the origin of the trauma, degree of injury, the presence of bone and dental fractures, and the occurrence of psychiatric disorders as a result of the trauma. RESULTS: This study demonstrated that maxillofacial traumas predominantly occurred in young male individuals, particularly among military personnel. The most common etiological factor identified was interpersonal violence. The majority of injuries were soft tissue damages, with the nasal bone being the most frequently fractured area. Injuries to the head and upper extremities were also detected in some of the cases, showing that multiple injuries are common in such cases. Post-traumatic psychological disorders developed in some cases, with anxiety disorders being the most commonly observed. CONCLUSION: It has been determined that maxillofacial injuries can affect multiple body regions, necessitating a multidisciplinary approach. This study underscores the importance of developing comprehensive strategies and policies for understanding and managing maxillofacial traumas, providing a fundamental reference for future studies in this field.

Fatal land hunting-related injuries in the Eastern Black Sea region-Turkey.

BACKGROUND: Hunting requires the use of various weapons or tools as professionals according to the characteristics of the creature to be hunted. Deaths during hunting activities may occur as a result of different reasons (firearm wounds, falling from a height, wild animal attack, or natural, etc.). These events' forensic reports are prepared by the physician who performed the first intervention. Identification of wounds, taking measurement photographs of the wounds before treatment, specifying the shape/sizes of foreign bodies in radiological imaging will be beneficial for determining the type of firearm, the number of shots, and the shooting distance. METHODS: The cases that performed autopsy between 2007 and 2016 at The Council of Forensic Medicine Trabzon Group Chairmanship were analyzed, retrospectively. Twenty-six (4.1%) deceased were examined. Crime scene investigation reports, hunting equipment, wound characteristics, and causes of death were investigated. RESULTS: All of our cases consisted of men (92.3%; n=24) with a mean age of 42.5 years (14-81; SD: ± 17.4). It was determined that 42.3% of the incidents occurred in winter, 80.8% (n=21) occurred in the daytime, 69.2% occurred in open areas such as fields, and 26.9% occurred in forested land. It was found that 88.5% of the deaths were caused by shotgun pellet/buckshot injuries, 7.7% (n=2) by falling off a cliff, and 3.8% by wild boar attacks (vascular injury). It was determined that 57.7% of the shots were made from long shooting distance, 19.2% were made from close range, and 11.5% were made from contact distance. It was found that 73.9% of the perpetrators were other hunters and 26.1% were the deceased person himself. It was determined that 42.3% of the injuries occurred in the chest and 38.4% in the head-and-neck region. It was determined that 96.2% of the cases did not undergo first aid intervention even though there were eyewitnesses in approximately ¾ of the cases. Ethyl alcohol was detected in only 3 cases (11.5%). CONCLUSION: It is thought that similar deaths can be prevented to a certain extent thanks to the hunting and medical first aid training to be given by the local governments. Risk control mechanisms must work properly. Besides, it is necessary to ensure that forensic experts can access the results of the proceedings in such cases. It is thought that this will be more beneficial in terms of developing new behavior styles in similar events.

Drowning in submerged cars caused by traffic accidents.

BACKGROUND: Traffic accidents are among the most common causes of death. A small proportion of drownings are associated with traffic accidents. The roads in the Eastern Black Sea Region, where the study was conducted are fairly close to the seas, rivers, and ponds. This study aims to evaluate the cases who underwent autopsies after the traffic accident between 2009 and 2016 and who were found to have died as a result of drowning. METHODS: A retrospective examination was made of the autopsy reports in the period 2009-2016. RESULTS: As a result of the examination of forensic reports, from a total of 7124 autopsies performed in our center between 2009 and 2016, 41 (0.57%) were seen to be due to death in a traffic accident that resulted in drowning. Of the vehicles involved in the ac-cidents, 30 (73.2%) were retrieved from a river/stream, 7 (17.1%) from a lake, and 4 (9.7%) from the sea. In all 39 cases, the primary cause of death was determined as asphyxia related to drowning. Other reasons affecting death were traumatic intracranial bleeding in 7 (17.1%) cases, medulla spinalis injury in 4 (9.7%), and pulmonary injury in 2 (4.9%). CONCLUSION: It was determined in the study that the typical autopsy results of trauma and drowning after a traffic accident could coexist. Drowning alone could be the cause of death, even though there was a traumatic origin such as a traffic accident in such cases. It was revealed that chemical and microscopic examinations should be handled together with crime scene results and eyewitness statements in addition to traumatic results during the examination phase.

Deaths due to 'patpat' accidents in the Black Sea Region, Turkey.

BACKGROUND: Overtime, agricultural machines have been designed appropriate to more simple needs, instead of tractors which have a wide area of use. An example of these is the 'patpat,' so named because of the sound of the motor, and for which the main purpose is ploughing. METHODS: A patpat is a single-axle, two-wheeled tractor with a trailer attached. In this descriptive study, the descriptive characteristics and causes of death were analyzed of case related to patpat accidents for which autopsies were performed by the Forensic Medicine Institution Trabzon Group Directorate between 2006 and 2017. RESULTS: Information was retrieved from the scene of accident examination documents, and the autopsy and laboratory records. The 21 cases comprised 17 (81%) males and 4 (19%) females, with a mean age of 47.6±20.3 years (median: 57, range: 10-75 years). Of the total accidents, in 85.7% (n=18), no other vehicle was involved. Following the accident, 52.4% (n=11) of the patpats were found at the side of the road, and 33.3% (n=7) at the edge of a stream or at the bottom of a steep valley. The cause of the accident was determined to be that the patpat had overturned on a bend in 33.3% (n=7) cases and in 85.7% (n=18) of cases, the patpat was found overturned or on its side. The most common injury was being thrown from the vehicle (38.1%, n=8), and in 66.7% (n=14) of cases, the deceased was the patpat driver. CONCLUSION: Patpat accidents are a serious problem because of the frequency of use of these vehicles without proper supervision and especially because of the hazardous topographical conditions where they are used.

Tuberculosis prevalence in forensic autopsies.

INTRODUCTION: According to the 2008 World Health Organization report, in 2006, 9.2 million new cases were determined, and 1.7 million people have lost their life due to tuberculosis (TB) in all around the world. In our country (Turkey), it is estimated that 35,000 to 40,000 people have TB disease annually. The Ministry of Health could just determine 18,500 of these cases, and only 6500 patient could be treated effectively. According to the Tuberculosis Dispensary records, the incidence for TB in Turkey is 28/100,000. MATERIALS AND METHODS: It is aimed to determine the infection with Mycobacterium tuberculosis using acidoresistant bacilli microscopy, TB culture, and histopathological methods in tissue samples that were obtained from lungs of forensic cases whose autopsies had been performed in Council of Forensic Medicine Ankara Department Morgue Specialized Committee. RESULTS: A total of 3 tissue samples that were obtained from lungs of randomized 302 cases, were positive for TB in Löwenstein-Jensen medium. Granuloma with caseating necrosis was found in histopathological examination and acidoresistant (+) bacilli (1+, 2+, and 2+, respectively) in microscopically analysis were also demonstrated in this 3 tissue samples. DISCUSSION: For this reason, we think that autopsy workers have to be careful about tuberculosis during their autopsy working.

Determining gender by taking measurements from magnetic resonance images of the patella.

BACKGROUND: A key step in making a positive identification in forensic medicine is the establishment of a biological profile, which involves determining factors such as gender, age, ancestry, and stature. The goal of this study was to determine if gender could be established by taking various measurements of the patella taken from magnetic resonance imaging (MRI) images and analyzing the variations by gender. METHODS: The sample group consisted of 220 patients (110 male and 110 female) whose patella were measured using MRI images of their left knee. Reasons for exclusion were any previous surgery, patella bipartite variation, any fracture in the patella due to trauma or findings of mass or infection. Three measurements - transverse length (TP), craniocaudal length (CC) and anteroposterior length (APP) - were taken off T2-weighted axial and sagittal MRI scans. The program SPSS (Version 21.0) was used to make a descriptive analysis, independent t-test and discriminative analysis. RESULTS: It was found possible to determine gender with an accuracy rate of 91% for females and 87% for males. Since measurements were made individually the accuracy for gender estimation is lower than that seen in other methods. CONCLUSION: The findings are important in that they show that it is possible to determine gender with a high degree of accuracy using just a few measurements taken from the patella.

The relationship between nonsuicidal self-injurious behavior and manners of death: a multicenter autopsy study

Background/aim: This study aimed to determine the relationship between nonsuicidal self-injurious behavior (NSSIB) and manners of death.Materials and methods: This study retrospectively evaluated 6604 autopsy cases evaluated at forensic medicine institutes of six major cities of Turkey. The study group consisted of all cases with NSSIB findings. The control group was created from cases without signs of NSSIB. Results: We found that the numbers of possible suicide and homicide cases in the NSSIB (+) group were significantly higher than in the NSSIB (-) group. The possibility of death due to suicide was 3.213 times and homicide was 2.004 times higher than natural deaths in the NSSIB (+) group compared with the NSSIB (-) group.Conclusion: The presence of NSSIB might increase the risk of death due to suicide and homicide, especially in adolescence.

Relationship Between Self-Injurious Behaviors and Levels of Aggression in Children and Adolescents Who Were Subject to Medicolegal Examination.

Aggression, which is defined as a behavior causing harm or pain, is a behavioral pattern typically expected in children and adolescents who are involved in criminal activities. The aim of this study was to examine the relationship between aggression and self-injurious behavior (SIB) in children and adolescents. The study was performed in 295 cases which were sent for medicolegal examination. The mean age of the subjects was 14.27 ± 1.05 years (age range 10-18 years). The aggression levels of the subjects were determined using the Aggression Questionnaire (AQ), which is an updated form of the Buss-Durkee Hostility Inventory. The mean total AQ score of the subjects with and without SIB was 78.04 ± 21.0 and 62.75 ± 18.05, respectively (p < 0.01). There were significant statistical differences between the two groups with respect to their subscale scores (p < 0.01). It was concluded that the levels of aggression increased in children and adolescents who were involved in criminal activities when the SIBs increased.

Electrocution fatalities in military personnel in Ankara, Turkey.

OBJECTIVES: To investigate various cases of death caused by electrical injuries among Turkish military personnel. METHODS: We retrospectively reviewed fatality cases of military personnel between 1994 and 2013 at the Department of Forensic Medicine, Gulhane Military Medical Academy, School of Medicine, Ankara, Turkey, the only forensic medicine center for the Turkish Armed Forces. Medical records and autopsy reports of cases of electrical fatalities were reviewed and analyzed in terms of age and gender-specific incidence, voltage, contact details, body region distribution, location, and season of incident, site, and severity of injuries sustained, and histopathological and toxicological findings. RESULTS: Sixteen (3.5%) out of the 450 autopsy cases involved electrocution. All deaths were accidental and most frequently occurred outdoors (75%). Eight (50%) died due to high voltage while 6 (37.5%) died due to low voltage. The entry and exit lesions were determined most frequently in cases with high voltage injury. The low voltage deaths commonly occurred at the scene of the event (66.6%), while almost all high voltage deaths occurred in the hospital (87.5%, p=0.03). Electrical burns were most commonly detected in the upper extremities (32.6%, n=14). CONCLUSION: The present study shows that deaths due to high voltage electrocution are more frequent than low voltage electrocution among military personnel.

Medical examiners and Crimean-Congo hemorrhagic fever contamination risk.

Crimean-Congo Hemorrhagic Fever (CCHF) is an acute zoonotic infection caused by the CCHF virus. The viruses' activity peaks during April and May with a mortality rate of 3-30%. Transmission of the virus to human occurs through tick bites or exposure to infected animals' tissues or blood. The major at-risk group includes farmers living in endemic areas. Health-care workers are the second most affected group. Virus has shown up in a diverse geographic area which includes Middle East, Asia, Africa and Eastern Europe and is considered one of the most wide-spread tick borne infections. The most recent cases are from Iran and Turkey. This article represents autopsy results of four CCHF infected cases in 2011 and 2012, in Ankara, Turkey.