Ultrasound-assisted extraction of scopolamine and hyoscyamine from Hyoscyamus niger roots using central compost design.

Hyoscyamus niger is an important medicinal plant used in medicine and contains tropane alkaloid compounds such as hyoscyamine and scopolamine. In this study, after the selection of the solvent for extracting hyoscyamine and scopolamine, the central composite design of the response surface methodology was used to study the effect of solvent concentrations (0, 25, 50, 75, and 100 %), temperatures (25, 30, 35, 40, and 45 °C) and ultrasonication times (10, 20, 30, 40, and 50 min). The hyoscyamine and scopolamine content were obtained by HPLC-DAD. The results indicated that the predicted optimal condition for hyoscyamine and scopolamine extraction from H. niger root was as follows. Hyoscyamine: 100 % methanol, temperature 45 °C and ultrasonication time 10 min, obtained 172.06 µg/g dry weight; and scopolamine: 98.50 % methanol, temperature 25 °C and ultrasonication time 10 min, provided 229.48 µg/g dry weight. To confirm the predicted extraction conditions, a separate experiment was conducted, and the results showed that the hyoscyamine and scopolamine contents were 164.72 and 209.23 µg/g dry weight, respectively.

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)

ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature embryos. There were significant differences between cultivars in terms of the percentage of callus induction and regeneration. Plant growth regulators had significant effect on percentage of callus induction in mature explants and percentage of regeneration from both explants. In immature explants, the highest percentage of regeneration (65%) was achieved with the Arta cultivar calli derived from MS medium supplemented with 1mg/L 2,4-D, 2 mg/L Picloram and 200 mg/L casein hydrolysate, and subcultured on MS medium. Also, the highest percentage of regeneration (52.38%) from mature embryo explants was achieved in the Arta cultivar with callus induction on MS medium supplemented with 1 mg/L 2,4-D, 2 mg/L Picloram and 200 mg/L casein hydrolysate and regeneration on MS medium containing 0.05 mg/L NAA.