Application of Ultrasonic Intelligent Imaging in L-Selectin Regulating Embryo Implantation in Mongolian Sheep Endometrium.

In order to explore the practical application of ultrasonic imaging in the pregnancy stage of Mongolian sheep and the role of L-selectin in the embryo implantation process of Mongolian sheep, this paper systematically observed the early embryonic development by B-mode ultrasonic imaging wave diagnostic instrument with 5 MHz rectal probe and detected the expression of sLex and L-selectin in embryonic cells (jar cells) and endometrial cells (RL95-2 cells) by immunoassay to show the role of L-selectin in embryonic adhesion. The results were as follows: the correct rate of fetal sex determination by ultrasound imaging increased with the increase of pregnancy days and reached 93% at 84 days; sLex/L-selectin on the surface of Jar/RL95-2 cells is involved in the adhesion between embryo and endometrium; and when the concentration of L-selectin was 30 µg/ml, the implantation success rate of fertilized eggs and embryos was the highest, reaching 95%. It is proved that ultrasonic intelligent imaging exploration can summarize the imaging characteristics of the early development law of sheep fetus, which provides a basis for B-ultrasound to monitor fetal growth and predict fetal age. While discussing the molecular mechanism of implantation, it provides a new idea and means for the clinical intervention of contraception and pregnancy assistance with oligosaccharide as the target.

[Characterization of expression of alpha1-acid glycoprotein gene in Beijing fatty chicken (Gallus gallus)].

The specific expression of alpha1-AGP gene in eight different tissues of Beijing fatty chicken was investigated by RT-PCR. The full-length cDNA of alpha1-AGP was inserted into pEGFP-C1 multi-cloning sites to construct recombinant eukaryotic expression vector pEGFP-alpha1-AGP. The lipofectin method was used to transfect the pEGFP-alpha1-AGP into Beijing fatty chicken fibroblast cells. The open reading frame of Beijing fatty chicken alpha1-AGP gene was 612 base pairs in length, which was expressed higher in liver and lung than in muscle. This gene did not express in heart and kidney. The expression efficiency ranged from 31.3% to 47.6% in 24, 48, and 72 h after transformation. The green fluorescence mainly concentrated in the nucleus. With the increase of the expression of green fluorescence, granula was observed in the nucleus. RT-PCR and Western blotting analyses showed that pEGFP-alpha1-AGP had been integrated into the genome of Beijing chicken fibroblast cell with normal expression level. In optimized condition, there was no significant effect (P>0.05) on apoptosis ratio, positive cell shape, growth and reduplication state comparing with the control group. This research established the foundation for further function research of alpha1-AGP gene and application in transgenic animal cloning.