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1.
Genome Res ; 16(6): 796-803, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16672307

RESUMO

Sequencing of full-insert clones from full-length cDNA libraries from both Xenopus laevis and Xenopus tropicalis has been ongoing as part of the Xenopus Gene Collection Initiative. Here we present 10,967 full ORF verified cDNA clones (8049 from X. laevis and 2918 from X. tropicalis) as a community resource. Because the genome of X. laevis, but not X. tropicalis, has undergone allotetraploidization, comparison of coding sequences from these two clawed (pipid) frogs provides a unique angle for exploring the molecular evolution of duplicate genes. Within our clone set, we have identified 445 gene trios, each comprised of an allotetraploidization-derived X. laevis gene pair and their shared X. tropicalis ortholog. Pairwise dN/dS, comparisons within trios show strong evidence for purifying selection acting on all three members. However, dN/dS ratios between X. laevis gene pairs are elevated relative to their X. tropicalis ortholog. This difference is highly significant and indicates an overall relaxation of selective pressures on duplicated gene pairs. We have found that the paralogs that have been lost since the tetraploidization event are enriched for several molecular functions, but have found no such enrichment in the extant paralogs. Approximately 14% of the paralogous pairs analyzed here also show differential expression indicative of subfunctionalization.


Assuntos
Sequência de Bases , Biblioteca Gênica , Poliploidia , Xenopus laevis/genética , Xenopus/genética , Animais , Evolução Molecular , Expressão Gênica , Genes Duplicados , Genoma , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Filogenia , Homologia de Sequência do Ácido Nucleico
2.
Proc Natl Acad Sci U S A ; 102(51): 18485-90, 2005 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-16352711

RESUMO

We analyzed 8.55 million LongSAGE tags generated from 72 libraries. Each LongSAGE library was prepared from a different mouse tissue. Analysis of the data revealed extensive overlap with existing gene data sets and evidence for the existence of approximately 24,000 previously undescribed genomic loci. The visual cortex, pancreas, mammary gland, preimplantation embryo, and placenta contain the largest number of differentially expressed transcripts, 25% of which are previously undescribed loci.


Assuntos
Perfilação da Expressão Gênica , Regulação da Expressão Gênica no Desenvolvimento/genética , Camundongos Endogâmicos C57BL/genética , Camundongos/genética , Processamento Alternativo/genética , Animais , Família Multigênica/genética , RNA não Traduzido/genética , Reprodutibilidade dos Testes , Transcrição Gênica/genética
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