Impact of surface roughness on Dielectrophoretically assisted concentration of microorganisms over PCB based platforms.

This article presents a PCB based microfluidic platform for performing a dielectrophoretic capture of live microorganisms over inter-digitated electrodes buried under layers of different surface roughness values. Although dielectrophoresis has been extensively studied earlier over silicon and polymer surfaces with printed electrodes the issue of surface roughness particularly in case of buried electrodes has been seldom investigated. We have addressed this issue through a layer of spin coated PDMS (of various surface roughness) that is used to cover the printed electrodes over a printed circuit board. The roughness in the PDMS layer is generally defined by the roughness of the FR4 base which houses the printed electrodes as well as other structures. Possibilities arising out of COMSOL simulations have been well validated experimentally in this work.

Virtually Administered Intervention Through Telerehabilitation for Chronic Non-specific Low Back Pain: A Review of Literature.

The most frequent reason for individuals to seek medical attention in both primary care settings and immediate care centers is low back pain (LBP). Over a duration of time, the disability caused by lower back pain has risen enough, particularly in countries with low or moderate incomes. In the coming years, there may be an increase in LBP-related impairment and expenses in countries with low or medium incomes, particularly when fragile medical systems are unable to handle this growing load. Hence, this review focuses on the effectiveness of telerehabilitation (TR) on LBP. The significant advantages of TR may include greater interaction and remote accessibility to medical treatments. The exchange of knowledge and health information is made possible through a more effective interaction, which benefits patients, families, carers, physicians, and researchers. People who live in distant places now have the opportunity to get medical attention assisting families in caring for patients with poor responsiveness. In addition, it provides the potential for prompt detection, the beginning of treatment in the midst of an emergency, a shorter stay in the hospital, ongoing monitoring of those at risk, and overall time and expense savings. Therefore, this study supports the application of TR in conditions of LBP for early management and relief of pain for patients in low-resource areas.

Telerehabilitation for a Non-specific Low Back Pain: A Case Report.

One of the most common conditions that affect activities of daily living and make them significantly more difficult to perform is low back pain (LBP). As a result, it is essential to treat LBP at an early stage. Particularly in geographically remote areas where there is a shortage of medical professionals and a lack of rehabilitation services, telerehabilitation is considered a potential alternative. Hence, this case report represents the impact of telerehabilitation on LBP in a 32-year-old female corporate worker who presented to the out-patient department of physiotherapy with the chief complaints of LBP for the last three months with difficulty in performing activities, and being unable to sit for prolonged period of time. The physiotherapeutic rehabilitation was virtually administered through online sessions through the cloud-based application as the patient was not able to visit the outpatient department on a regular basis. Post-intervention results demonstrated increased range of motion and flexibility, reduced pain, increased muscle strength, reduced disability and kinesiophobia, and improved quality of life. Hence, it can be concluded that telerehabilitation offers a novel solution to increase access to rehabilitation services.

Stability of carbon quantum dots: a critical review.

Carbon quantum dots (CQDs) are fluorescent carbon nanomaterials with unique optical and structural properties that have drawn extensive attention from researchers in the past few decades. Environmental friendliness, biocompatibility and cost effectiveness of CQDs have made them very renowned in countless applications including solar cells, white light-emitting diodes, bio-imaging, chemical sensing, drug delivery, environmental monitoring, electrocatalysis, photocatalysis and other related areas. This review is explicitly dedicated to the stability of CQDs under different ambient conditions. Stability of CQDs is very important for every possible application and no review has been put forth to date that emphasises it, to the best of our knowledge. This review's primary goal is to make the readers cognizant of the importance of stability, ways to assess it, factors that affect it and proposed ways to enhance the stability for making CQDs suitable for commercial applications.

Integrated DEP Assisted Detection of PCR Products With Metallic Nanoparticle Labels Through Impedance Spectroscopy.

Electrochemical impedance spectroscopy (EIS) is gaining immense popularity in the current times due to the ease of integration with microelectronics. Keeping this aspect in mind, various detection schemes have been developed to make impedance detection of nucleic acids more specific. In this context, the current work makes a strong case for specific DNA detection through EIS using nanoparticle labeling approach and also an added selectivity step through the use of dielectrophoresis (DEP), which enhances the detection sensitivity and specificity to match the detection capability of quantitative polymerase chain reaction (qPCR) in real-time context as compared to the individually amplified DNA (Liu et al., 2008). The detection limit of the proposed biochip is observed to be 3-4 PCR cycles for 582 bp bacterial DNA, where the complete procedure of detection starts in less than 10 min. The process of integrated DEP capture of labeled products coming out of PCR and their impedance-assisted detection is carried out in an in-house micro-fabricated biochip. The gold nanoparticles, which possess excellent optical, chemical, electronic, and biocompatibility properties and are capable of generating lump-like DNA structure without modifying its basic impedance signature are introduced to the amplified DNA through the nanoparticle labeled primers.

A review on advancements in carbon quantum dots and their application in photovoltaics.

Carbon quantum dots are a new frontier in the field of fluorescent nanomaterials, and they exhibit fascinating properties such as biocompatibility, low toxicity, eco-friendliness, good water solubility and photostability. In addition, the synthesis of these nanoparticles is facile, rapid, and satisfies green chemistry principles. CQDs have easily tunable optical properties and have found applications in bioimaging, nanomedicine, drug delivery, solar cells, light-emitting diodes, photocatalysis, electrocatalysis and other related areas. This article systematically reviews carbon quantum dot structure, their synthesis techniques, recent advancements, the effects of doping and surface engineering on their optical properties, and related photoluminescence models in detail. The challenges associated with these nanomaterials and their prospects are discussed, and special emphasis has been placed on the application of carbon quantum dots in enhancing the performance of photovoltaics and white light-emitting diodes.

Correction: A review on advancements in carbon quantum dots and their application in photovoltaics.

[This corrects the article DOI: 10.1039/D1RA08452F.].

Synchronized Electromechanical Shock Wave-Induced Bacterial Transformation.

We report a simple device that generates synchronized mechanical and electrical pressure waves for carrying out bacterial transformation. The mechanical pressure waves are produced by igniting a confined nanoenergetic composite material that provides ultrahigh pressure. Further, this device has an arrangement through which a synchronized electric field (of a time-varying nature) is initiated at a delay of ≈85 µs at the full width half-maxima point of the pressure pulse. The pressure waves so generated are incident to a thin aluminum-polydimethylsiloxane membrane that partitions the ignition chamber from the column of the mixture containing bacterial cells (Escherichia coli BL21) and 4 kb transforming DNA. A combination of mechanical and electrical pressure pulse created through the above arrangement ensures that the transforming DNA transports across the cell membrane into the cell, leading to a transformation event. This unique device has been successfully operated for efficient gene (∼4 kb) transfer into cells. The transformation efficacy of this device is found comparable to the other standard methods and protocols for carrying out the transformation.