'Setting the Benchmark' Part 2: Contextualising the Physical Demands of Teams in the FIFA World Cup Qatar 2022.

This study aimed to contextualise and benchmark the physical demands of teams in the FIFA World Cup Qatar 2022. With FIFA's official approval, all sixty-four games were analysed during the competition (n = 32 teams) using a multi-camera computerised tracking system. On average, teams during Qatar 2022 covered around 108.1 ± 3.6 km in total, with 9.0 ± 0.9 and 2.3 ± 0.3 km covered at the higher intensities (≥20.0 and ≥25.0 km · h-1), respectively. Compared to the FIFA World Cup Russia 2018, national teams in Qatar 2022 covered only 3% more total distance but 16-19% more distance at the higher intensities (P < 0.01; Effect Size [ES]: 0.9-2.0). When the data was adjusted based on the number of minutes played, tournament differences at the higher intensities were less pronounced (9-12%; P < 0.01; ES: 0.7-1.3). The United States, Canada, Saudi Arabia, Germany and IR Iran covered 19-34% more high-intensity distance than Argentina, Ecuador, Qatar, Poland and Costa Rica during the 2022 tournament (P < 0.01; ES: 3.2-3.5). Match-to-match variation of each team in Qatar 2022 revealed Ecuador and Uruguay were particularly consistent for the distances covered at higher intensities (Coefficient of Variation [CV]: 2-3%), whilst Japan demonstrated considerable variation (CV: 23-29%). Teams generally covered more total distance on a per-minute basis in the first versus the second half (P < 0.01; ES: 1.2), but no differences existed at higher intensities (P > 0.05; ES: 0.0-0.1). Correlations between the number of high-intensity runs and various phase of play events across all teams were strongest for defensive transitions and recoveries, in addition to progressions up the pitch and into the final third (r = 0.63-0.75; P < 0.01). The present findings provide valuable context into the contemporary team demands of international football. This information could be useful for practitioners to benchmark team performances and to potentially understand the myriad of factors impacting physical performances.

'Setting the Benchmark' Part 1: The Contextualised Physical Demands of Positional Roles in the FIFA World Cup Qatar 2022.

This study aimed to contextualise and benchmark the positional role demands during the FIFA World Cup Qatar 2022. With FIFA's official approval, all sixty-four games were analysed during the competition (n=722) using a multi-camera computerised tracking system. During a typical FIFA World Cup Qatar 2022 match, defensive and central midfielders covered 8-19% more total distance than other positional roles (P<0.01; Effect Size [ES]: 0.8-2.5). The distances covered at higher intensities (≥20 and ≥25 km · h-1) were 16-92% and 36-138% higher for wide midfielders and wide forwards compared to central defenders, defensive and central midfielders as well as centre forwards (P<0.01; ES: 0.7-2.2 and ES: 0.6-1.4). Defensive and central midfielders covered a greater proportion of their distance at higher intensities (≥20 and ≥25 km · h-1) out-of-possession (71-83%; P<0.01; ES: 1.4-3.0), whilst attacking midfielders, wide and centre forwards more in-possession (55-68%; P<0.01; ES: 1.6-3.3). Nine out of the top ten sprint speeds attained at the tournament were from wide positional roles (35.3-35.7 km · h-1). All positional roles demonstrated a second half reduction in total distance covered compared to the first half (P<0.01; ES: 0.8-1.3). A decline between halves for the distances covered at higher intensities (≥20 and ≥25 km · h-1) were more evident in attacking midfielders, wide defenders and midfielders than for other positional roles (P<0.01; ES: 0.3-0.7). Defensive midfielders and centre forwards were found to have the highest coefficient of variation (CV: 30.9-35.9% and 67.7-67.8%) for the distances covered at higher intensities (≥20 and ≥25 km · h-1) compared to other positional roles. The current findings provide valuable contextual information about the contemporary positional demands of international football. This could be useful in the development and prescription of specific training regimes for national teams.

Contaminant Exposure and Transport from Three Potential Reuse Waters within a Single Watershed.

Global demand for safe and sustainable water supplies necessitates a better understanding of contaminant exposures in potential reuse waters. In this study, we compared exposures and load contributions to surface water from the discharge of three reuse waters (wastewater effluent, urban stormwater, and agricultural runoff). Results document substantial and varying organic-chemical contribution to surface water from effluent discharges (e.g., disinfection byproducts [DBP], prescription pharmaceuticals, industrial/household chemicals), urban stormwater (e.g., polycyclic aromatic hydrocarbons, pesticides, nonprescription pharmaceuticals), and agricultural runoff (e.g., pesticides). Excluding DBPs, episodic storm-event organic concentrations and loads from urban stormwater were comparable to and often exceeded those of daily wastewater-effluent discharges. We also assessed if wastewater-effluent irrigation to corn resulted in measurable effects on organic-chemical concentrations in rain-induced agricultural runoff and harvested feedstock. Overall, the target-organic load of 491 g from wastewater-effluent irrigation to the study corn field during the 2019 growing season did not produce substantial dissolved organic-contaminant contributions in subsequent rain-induced runoff events. Out of the 140 detected organics in source wastewater-effluent irrigation, only imidacloprid and estrone had concentrations that resulted in observable differences between rain-induced agricultural runoff from the effluent-irrigated and nonirrigated corn fields. Analyses of pharmaceuticals and per-/polyfluoroalkyl substances in at-harvest corn-plant samples detected two prescription antibiotics, norfloxacin and ciprofloxacin, at concentrations of 36 and 70 ng/g, respectively, in effluent-irrigated corn-plant samples; no contaminants were detected in noneffluent irrigated corn-plant samples.

Transcranial direct current stimulation during a prolonged cognitive task: the effect on cognitive and shooting performances in professional female basketball players.

The negative effect of prolonged cognitive demands on psychomotor skills in athletes has been demonstrated. Transcranial direct current stimulation (tDCS) could be used to mitigate this effect. This study examined the effects of tDCS over the left dorsolateral prefrontal cortex (DLPFC) during a 30-min inhibitory Stroop task on cognitive and shooting performances of professional female basketball players. Following a randomised, double-blinded, sham-controlled, cross-over design, players were assigned to receive anodal tDCS (a-tDCS, 2 mA for 20 min) or sham-tDCS in two different sessions. Data from 8 players were retained for analysis. Response Time decreased significantly over time (p < 0.001; partial η2 = 0.44; no effect of condition, or condition vs. time interaction). No difference in mean accuracy and shooting performance was observed between tDCS conditions. The results suggest that a-tDCS exert no additional benefits in reducing the negative effects of prolonged cognitive demands on technical performance compared to sham (placebo).Practitioner summary: Prolonged cognitive demands can negatively affect the athletes' performance. We tested whether transcranial direct current stimulation (tDCS) over the left dorsolateral prefrontal cortex (DLPFC) could attenuate these effects on cognitive and shooting performance in professional female basketball players. However, tDCS did not exert any additional benefits compared to sham.Abbreviations: tDCS: transcranial direct current stimulation; a-tDCS: anodal transcranial direct current stimulation; PFC: prefrontal cortex; DLPFC: dorsolateral prefrontal cortex; PCT: prolonged cognitive task; TT: time trial; RT: response time; NASA-TLX: National Aeronautics and Space Administration Task Load Index; RPE: ratings of perceived exertion; CR-10 scale: category rating scale; EEG: electroencephalogram; AU: arbitrary units.

Contextualised high-intensity running profiles of elite football players with reference to general and specialised tactical roles.

The present study aimed to contextualise physical metrics with tactical actions according to general and specialised tactical roles. A total of 244 English Premier League players were analysed by coding player's physical-tactical actions via the fusion of tracking data and video. Data were analysed across 5 general (Central Defensive Players = CDP, Wide Defensive Players = WDP, Central Midfield Players = CMP, Wide Offensive Players = WOP, Central Offensive Players = COP) and 11 specialised positions (Centre Backs = CB, Full-Backs = FB, Wing-Backs = WB, Box-to-Box Midfielders = B2BM, Central Defensive Midfielders = CDM, Central Attacking Midfielders = CAM, Wide Midfielders = WM, Wide Forwards = WF, Centre Forwards = CF). COP covered more distance at high-intensity (> 19.8 km · h-1) when performing actions such as 'Break into Box', Run in Behind/Penetrate', and 'Close Down/Press' than other positions (ES: 0.6-5.2, P < 0.01). WOP covered more high-intensity 'Run with Ball' distance (ES: 0.7-1.7, P < 0.01) whereas WDP performed more 'Over/Underlap' distance than other positions (ES: 0.9-1.4, P < 0.01). CDP and WDP covered more high-intensity 'Covering' distances than other positions (ES: 0.4-2.4, P < 0.01). Nonetheless, data demonstrated that implementing specialised positional analysis relative to a generalised approach is more sensitive in measuring physical-tactical performances of players with the latter over or underestimating the match demands of the players compared to the former. A contextualised analysis may assist coaches and practitioners when designing position or even player-specific training drills since the data provides unique physical-tactical trends across specialised roles.

Food, Beverage, and Feedstock Processing Facility Wastewater: a Unique and Underappreciated Source of Contaminants to U.S. Streams.

Process wastewaters from food, beverage, and feedstock facilities, although regulated, are an under-investigated environmental contaminant source. Food process wastewaters (FPWWs) from 23 facilities in 17 U.S. states were sampled and documented for a plethora of chemical and microbial contaminants. Of the 576 analyzed organics, 184 (32%) were detected at least once, with concentrations as large as 143 µg L-1 (6:2 fluorotelomer sulfonic acid), and as many as 47 were detected in a single FPWW sample. Cumulative per/polyfluoroalkyl substance concentrations up to 185 µg L-1 and large pesticide transformation product concentrations (e.g., methomyl oxime, 40 µg L-1; clothianidin TMG, 2.02 µg L-1) were observed. Despite 48% of FPWW undergoing disinfection treatment prior to discharge, bacteria resistant to third-generation antibiotics were found in each facility type, and multiple bacterial groups were detected in all samples, including total coliforms. The exposure-activity ratios and toxicity quotients exceeded 1.0 in 13 and 22% of samples, respectively, indicating potential biological effects and toxicity to vertebrates and invertebrates associated with the discharge of FPWW. Organic contaminant profiles of FPWW differed from previously reported contaminant profiles of municipal effluents and urban storm water, indicating that FPWW is another important source of chemical and microbial contaminant mixtures discharged into receiving surface waters.

The validity and reliability of an integrated approach for quantifying match physical-tactical performance.

This study aimed to: (1) develop an integrated approach to quantifying match physical-tactical performance and (2) comprehensively examine the validity and reliability of this novel approach. Both UEFA qualified coaches and performance analysts (n = 30) participated to verify the scientific robustness of this new method. The percentage of correct responses were used to verify the validity of the integrated approach and the minimum acceptable agreement was set at 80%. Two well-trained groups of observers analysed a randomly selected English Premier League match for inter- and intra-observer reliability using the kappa statistic. A high degree of validity was demonstrated as the mean percentage of correct responses by all participants, accounting for 91.8 ± 4.3% for all, 92.2 ± 4.7% for out-of-possession, and 91.6 ± 5.7% for in-possession physical-tactical variables. Inter- and intra-observer reliability were found to be strong (κ = 0.81) to almost perfect (κ = 0.94), respectively. Additional analyses demonstrated that there was a nearly perfect correlation between data derived from the novel filter used for the present study to capture high-intensity running and those obtained from the filter of the commercial data provider (r = 0.99; P < 0.01). The data demonstrates that the integrated approach is valid and reliable regarding the quantification of physical-tactical performances. Therefore, it is now possible to unveil unique high-intensity profiles of elite players related to key tactical actions. This may help coaches and practitioners better understand the physical-tactical performances of players, as well as effectively translate physical metrics into training.

Accumulative weekly load in a professional football team: with special reference to match playing time and game position.

The aim of this study was to compare weekly accumulative load during the in-season competitive period by professional soccer players according to the amount of time played in official matches (90-min, >60-min, <60-min, and 0-min) regarding the players' position. Twenty-four professional outfield football players were monitored by GPS devices during training sessions and official matches and the accumulative weekly load were calculated for the following external load variables: total distance (TD; m), high speed running (HSR; >19.8 km·h-1), sprint meters (SPR; >25.2 km·h-1), high metabolic load distance (HMLD; >25.5 W·kg-1), number of accelerations (ACC; >3 m·s-2) and decelerations (DEC; <-3 m·s-2). This study revealed that players (as a whole o per demarcation) with more match playing time had a higher accumulative weekly load for most of the variables, but particularly at TD and HMLD (90-min and >60-min vs. <60-min and 0-min), HSR (90-min vs. <60-min and 0-min) and SPR (90-min vs. <60-min and 0-min). In addition, less positional variation was observed in relation to the competition requirements. This information would allow coaches to refine the required load with the objective of optimizing performance to soccer players.

Contextualised peak periods of play in English Premier League matches.

The present study aimed to determine the physical-tactical trends of elite players/teams during peak 1-, 3- and 5-min periods of match-play. A total of 50 English Premier League matches (n = 583 player observations) were analysed by coding the players' physical-tactical activities through the synchronisation of tracking data and video. The contextualised data showed that during the peak periods (i.e., the most demanding passage of play), players/teams covered the largest distances for 'Recovery Run' (28-37%) out of possession and 'Support Play' (9-13%) in possession. In the following periods, players covered less high-intensity distance versus the average with a more pronounced decline in the next 1-min period than longer duration 3- and 5-min periods (48% vs ~25-30%, ES: 0.4-0.5, P < 0.01); team data showed similar trends with different relative patterns (31% vs 17-30%, ES: 0.5-0.8, P < 0.01). After peak periods, players/teams performed 20-53% less high-intensity distances for 'Covering' and 'Recovery Run' (ES: 0.2-0.7, P < 0.01) out of possession. However, players covered 28-91% less distance for 'Run with Ball' (ES: 0.1-0.5, P < 0.05) when in possession. Some physical-tactical actions exhibited inconsistency in different time durations of the next periods; however, these physical-tactical data were position-specific. This may signify that each position has certain physical-tactical actions to execute even after the peak periods, especially when they are tactically required to do so. As the data demonstrates unique physical-tactical trends of players/teams during the peak and next periods of play, this could help practitioners prescribe position- and player-specific drills, and better understand transient decrements in high-intensity running after intense passages of play.

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams.

Surface waters often contain a variety of chemical contaminants potentially capable of producing adverse outcomes in both humans and wildlife due to impacts from industrial, urban, and agricultural activity. Here, we report the results of a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological effects of complex contaminant mixtures using water collected from eight impacted streams across the United States mainland and Puerto Rico. We initially characterized the ZFL lipidome using high resolution mass spectrometry, resulting in the annotation of 508 lipid species covering 27 classes. We then identified lipid changes induced by all streamwater samples (nonspecific stress indicators) as well as those unique to water samples taken from specific streams. Subcellular impacts were classified based on organelle-specific lipid changes, including increased lipid saturation (endoplasmic reticulum stress), elevated bis(monoacylglycero)phosphate (lysosomal overload), decreased ubiquinone (mitochondrial dysfunction), and elevated ether lipids (peroxisomal stress). Finally, we demonstrate how these results can uniquely inform environmental monitoring and risk assessments of surface waters.

Inclusion of Pesticide Transformation Products Is Key to Estimating Pesticide Exposures and Effects in Small U.S. Streams.

Improved analytical methods can quantify hundreds of pesticide transformation products (TPs), but understanding of TP occurrence and potential toxicity in aquatic ecosystems remains limited. We quantified 108 parent pesticides and 116 TPs in more than 3 700 samples from 442 small streams in mostly urban basins across five major regions of the United States. TPs were detected nearly as frequently as parents (90 and 95% of streams, respectively); 102 TPs were detected at least once and 28 were detected in >20% samples in at least one region-TPs of 9 herbicides, 2 fungicides (chlorothalonil and thiophanate-methyl), and 1 insecticide (fipronil) were the most frequently detected. TPs occurred commonly during baseflow conditions, indicating chronic environmental TP exposures to aquatic organisms and the likely importance of groundwater as a TP source. Hazard quotients based on acute aquatic-life benchmarks for invertebrates and nonvascular plants and vertebrate-centric molecular endpoints (sublethal effects) quantify the range of the potential contribution of TPs to environmental risk and highlight several TP exposure-response data gaps. A precautionary approach using equimolar substitution of parent benchmarks or endpoints for missing TP benchmarks indicates that potential aquatic effects of pesticide TPs could be underestimated by an order of magnitude or more.

Machine Learning Models of Arsenic in Private Wells Throughout the Conterminous United States As a Tool for Exposure Assessment in Human Health Studies.

Arsenic from geologic sources is widespread in groundwater within the United States (U.S.). In several areas, groundwater arsenic concentrations exceed the U.S. Environmental Protection Agency maximum contaminant level of 10 µg per liter (µg/L). However, this standard applies only to public-supply drinking water and not to private-supply, which is not federally regulated and is rarely monitored. As a result, arsenic exposure from private wells is a potentially substantial, but largely hidden, public health concern. Machine learning models using boosted regression trees (BRT) and random forest classification (RFC) techniques were developed to estimate probabilities and concentration ranges of arsenic in private wells throughout the conterminous U.S. Three BRT models were fit separately to estimate the probability of private well arsenic concentrations exceeding 1, 5, or 10 µg/L whereas the RFC model estimates the most probable category (≤5, >5 to ≤10, or >10 µg/L). Overall, the models perform best at identifying areas with low concentrations of arsenic in private wells. The BRT 10 µg/L model estimates for testing data have an overall accuracy of 91.2%, sensitivity of 33.9%, and specificity of 98.2%. Influential variables identified across all models included average annual precipitation and soil geochemistry. Models were developed in collaboration with public health experts to support U.S.-based studies focused on health effects from arsenic exposure.

Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement.

Monitoring of the Colorado River near the Moab, Utah, wastewater treatment plant (WWTP) outflow has detected pharmaceuticals, hormones, and estrogen-receptor (ER)-, glucocorticoid receptor (GR)-, and peroxisome proliferator-activated receptor-gamma (PPARγ)-mediated biological activities. The aim of the present multi-year study was to assess effects of a WWTP replacement on bioactive chemical (BC) concentrations. Water samples were collected bimonthly, pre- and post-replacement, at 11 sites along the Colorado River upstream and downstream of the WWTP and analyzed for in vitro bioactivities (e.g., agonism of ER, GR, and PPARγ) and BC concentrations; fathead minnows were cage deployed pre- and post-replacement at sites with varying proximities to the WWTP. Before the WWTP replacement, in vitro ER (24 ng 17ß-estradiol equivalents/L)-, GR (60 ng dexamethasone equivalents/L)-, and PPARγ-mediated activities were detected at the WWTP outflow but diminished downstream. In March 2018, the WWTP effluent was acutely toxic to the fish, likely due to elevated ammonia concentrations. Following the WWTP replacement, ER, GR, and PPARγ bioactivities were reduced by approximately 60-79%, no toxicity was observed in caged fish, and there were marked decreases in concentrations of many BCs. Results suggest that replacement of the Moab WWTP achieved a significant reduction in BC concentrations to the Colorado River.

Are There Differences in Elite Youth Soccer Player Work Rate Profiles in Congested vs. Regular Match Schedules?

ABSTRACT: Zanetti, V, Carling, C, Aoki, MS, Bradley, PS, and Moreira, A. Are there differences in elite youth soccer player work rate profiles in congested vs. regular match schedules? J Strength Cond Res 35(2): 473-480, 2021-Official international tournaments in which youth soccer players participate can involve very congested schedules. Yet, no information regarding physical and technical match performance during congested vs. regular (noncongested) cycles is available. In this study, accelerations, decelerations, mean metabolic power (MP), and technical performance (offensive and defensive variables) were compared across very congested match (VCM; 10 international matches played over 3 successive days, including 2 days with 2 consecutive matches separated by a 4- to 5-hour interval) and 10 regular (noncongested match [NCM]) match periods in elite male Under 15 (U15, n = 11) and Under 17 (U17, n = 13) soccer players. Players wore a 15-Hz Global Positioning System unit with a 100-Hz triaxial accelerometer. The session rating of perceived exertion (RPE) were assessed 30 minutes after match. Results showed a higher number of accelerations per minute observed in VCM vs. NCM (U15; 2.27 ± 0.35 vs. 2.12 ± 0.23; effect size [ES] = 0.49; U17; 2.27 ± 0.41 vs. 2.01 ± 0.31; ES = 0.69). Decelerations per minute were higher during VCM (U15; 1.99 ± 0.27 vs. 1.84 ± 0.25; ES = 0.55; and U17; 1.98 ± 0.35 vs. 1.80 ± 0.27; ES = 0.56). Mean MP was higher in the VCM (U15; 0.42 ± 0.06 vs. 0.37 ± 0.02; ES = 1.08; U17; 0.46 ± 0.03 vs. 0.30 ± 0.03; ES = 1.94). Technical actions per minute were higher in the VCM for U17 (ES = 1.60 and 1.37, for offensive and defensive performance, respectively) but lower (during VCM) for U15 (ES = 3.59 and 0.28, for offensive and defensive performance). U15 reported a higher session RPE in the VCM (7.9 ± 0.5 AUs vs. 6.9 ± 0.5 AUs). The findings suggest that running activity in these youth players was unaffected overall in tournaments with congested schedules, and that the intensity of match-play was actually greater than in regular match schedules.

Methylmercury-total mercury ratios in predator and primary consumer insects from Adirondack streams (New York, USA).

Mercury (Hg) is a global pollutant that affects biota in remote settings due to atmospheric deposition of inorganic Hg, and its conversion to methylmercury (MeHg), the bioaccumulating and toxic form. Characterizing biotic MeHg is important for evaluating aquatic ecosystem responses to changes in Hg inputs. Aquatic insects possess many qualities desired for MeHg biomonitoring, but are not widely used, largely because of limited information regarding percentages of total mercury (THg) composed of MeHg (i.e., MeHg%) in various taxa. Here, we examine taxonomic, spatial, and seasonal variation in MeHg% of stream-dwelling predator and primary-consumer insects from nine streams in the Adirondack region (NY, USA). Predator MeHg% was high (median 94%) and did not differ significantly among five taxa. MeHg% in selected dragonflies (the most abundant predators, Odonata: Aeshnidae and Libellulidae) exhibited little seasonal and spatial variation, and THg concentration was strongly correlated with aqueous (filtered) MeHg (FMeHg; rs = 0.76). In contrast, MeHg% in primary consumers-shredders (northern caddisflies [Trichoptera: Limnephilidae]) and scrapers (flathead mayflies [Ephemeroptera: Heptageniidae]), were lower (medians 52% and 35%, respectively), and differed significantly between taxa, among sites, and seasonally. Correlations of THg with FMeHg were weak (shredders, rs = 0.45, p = 0.09) or not significant (scrapers, p = 0.89). The higher MeHg% of predators corresponded with their higher trophic positions (indicated by nitrogen stable isotopes). Results suggest obligate predators hold the most promise for the use of THg as a surrogate for MeHg biomonitoring with aquatic insects within the Adirondack region.

Soccer-Specific Reactive Repeated-Sprint Ability in Elite Youth Soccer Players: Maturation Trends and Association With Various Physical Performance Tests.

Di Mascio, M, Ade, J, Musham, C, Girard, O, and Bradley, PS. Soccer-specific reactive repeated-sprint ability in elite youth soccer players: maturation trends and association with various physical performance tests. J Strength Cond Res 34(12): 3538-3545, 2020-Repeated-sprint ability is an important physical prerequisite for competitive soccer and deviates for players in various stages of growth and development. Thus, this study investigated reactive repeated-sprint ability in elite youth soccer players in relation to maturation (age at peak height velocity) and its association with performance of other physical tests. Elite male youth players from an English Premier League academy (U12, n = 8; U13, n = 11; U14, n = 15; U15, n = 6; U16, n = 10; and U18, n = 13) completed the reactive repeated-sprint test (RRST; 8 × 30-m sprints with 30-second active recovery), and other physical tests including the Yo-Yo intermittent recovery test level 2 (Yo-Yo IR2), arrowhead agility test, countermovement jump test with arms (CMJA), in addition to 10- and 20-m straight-line sprints. Reactive repeated-sprint test (RRST) performance (total time across 8 sprints) progressively improved from U12 to U16 (p < 0.01; effect size [ES]: 1.0-1.9), yet with no differences found between U16 and U18. No between-group differences in RRST performance were evident after accounting for age at peak height velocity (p > 0.05; ES: <0.3). Correlation magnitudes between performance on the RRST and other tests were trivial to moderate for the Yo-Yo IR2 (r = -0.15 to 0.42), moderate to very large for the arrowhead agility test (r = 0.48-0.90), moderate to large for CMJA (r = -0.43 to 0.66), and trivial to large for 10- and 20-m sprints (r = 0.05-0.61). The RRST was sensitive at tracking maturation trends in elite youth players, although performance improvements were not as marked from 15 to 16 years of age. RRST performance correlates with several physical qualities decisive for competitive soccer (agility, speed, power, and aerobic endurance).

Cell-Based Metabolomics for Untargeted Screening and Prioritization of Vertebrate-Active Stressors in Streams Across the United States.

The U.S. Geological Survey and the U.S. Environmental Protection Agency have assessed contaminants in 38 streams across the U.S., using an extensive suite of target-chemical analysis methods along with a variety of biological effects tools. Here, we report zebrafish liver (ZFL) cell-culture based NMR metabolomic analysis of these split stream samples. We used this untargeted approach to evaluate the sites according to overall impact on the ZFL metabolome and found that neither the total number of organics detected at the sites, nor their cumulative concentrations, were good predictors of these impacts. Further, we used partial least squares regression to compare ZFL endogenous metabolite profiles to values for 455 potential stressors (organics, inorganics, and physical properties) measured in these waters and found that the profiles covaried with at most 280 of the stressors, which were subsequently ranked into quartiles based on the strength of their covariance. While contaminants of emerging concern (CECs) were well represented in the top, most strongly covarying quartile-suggesting considerable potential for eliciting biological responses at these sites-there was even higher representation of various well-characterized legacy contaminants (e.g., PCBs). These results emphasize the importance of complementing chemical analysis with untargeted bioassays to help focus regulatory efforts on the most significant ecosystem threats.

Potential Toxicity of Complex Mixtures in Surface Waters from a Nationwide Survey of United States Streams: Identifying in Vitro Bioactivities and Causative Chemicals.

While chemical analysis of contaminant mixtures remains an essential component of environmental monitoring, bioactivity-based assessments using in vitro systems increasingly are used in the detection of biological effects. Historically, in vitro assessments focused on a few biological pathways, for example, aryl hydrocarbon receptor (AhR) or estrogen receptor (ER) activities. High-throughput screening (HTS) technologies have greatly increased the number of biological targets and processes that can be rapidly assessed. Here we screened extracts of surface waters from a nationwide survey of United States streams for bioactivities associated with 69 different end points using two multiplexed HTS assays. Bioactivity of extracts from 38 streams was evaluated and compared with concentrations of over 700 analytes to identify chemicals contributing to observed effects. Eleven primary biological end points were detected. Pregnane X receptor (PXR) and AhR-mediated activities were the most commonly detected. Measured chemicals did not completely account for AhR and PXR responses. Surface waters with AhR and PXR effects were associated with low intensity, developed land cover. Likewise, elevated bioactivities frequently associated with wastewater discharges included endocrine-related end points ER and glucocorticoid receptor. These results underscore the value of bioassay-based monitoring of environmental mixtures for detecting biological effects that could not be ascertained solely through chemical analyses.

Predictive Analysis Using Chemical-Gene Interaction Networks Consistent with Observed Endocrine Activity and Mutagenicity of U.S. Streams.

In a recent U.S. Geological Survey/U.S. Environmental Protection Agency study assessing more than 700 organic compounds in 38 streams, in vitro assays indicated generally low estrogen, androgen, and glucocorticoid receptor activities, with 13 surface waters with 17ß-estradiol-equivalent (E2Eq) activities greater than a 1-ng/L estimated effects-based trigger value for estrogenic effects in male fish. Among the 36 samples assayed for mutagenicity in the Salmonella bioassay (reported here), 25% had low mutagenic activity and 75% were not mutagenic. Endocrine and mutagenic activities of the water samples were well correlated with each other and with the total number and cumulative concentrations of detected chemical contaminants. To test the predictive utility of knowledge-base-leveraging approaches, site-specific predicted chemical-gene (pCGA) and predicted analogous pathway-linked (pPLA) association networks identified in the Comparative Toxicogenomics Database were compared with observed endocrine/mutagenic bioactivities. We evaluated pCGA/pPLA patterns among sites by cluster analysis and principal component analysis and grouped the pPLA into broad mode-of-action classes. Measured E2eq and mutagenic activities correlated well with predicted pathways. The pPLA analysis also revealed correlations with signaling, metabolic, and regulatory groups, suggesting that other effects pathways may be associated with chemical contaminants in these waters and indicating the need for broader bioassay coverage to assess potential adverse impacts.

Urban Stormwater: An Overlooked Pathway of Extensive Mixed Contaminants to Surface and Groundwaters in the United States.

Increasing global reliance on stormwater control measures to reduce discharge to surface water, increase groundwater recharge, and minimize contaminant delivery to receiving waterbodies necessitates improved understanding of stormwater-contaminant profiles. A multiagency study of organic and inorganic chemicals in urban stormwater from 50 runoff events at 21 sites across the United States demonstrated that stormwater transports substantial mixtures of polycyclic aromatic hydrocarbons, bioactive contaminants (pesticides and pharmaceuticals), and other organic chemicals known or suspected to pose environmental health concern. Numerous organic-chemical detections per site (median number of chemicals detected = 73), individual concentrations exceeding 10 000 ng/L, and cumulative concentrations up to 263 000 ng/L suggested concern for potential environmental effects during runoff events. Organic concentrations, loads, and yields were positively correlated with impervious surfaces and highly developed urban catchments. Episodic storm-event organic concentrations and loads were comparable to and often exceeded those of daily wastewater plant discharges. Inorganic chemical concentrations were generally dilute in concentration and did not exceed chronic aquatic life criteria. Methylmercury was measured in 90% of samples with concentrations that ranged from 0.05 to 1.0 ng/L.