Resilient yet entirely degradable gelatin-based biogels for soft robots and electronics.

Biodegradable and biocompatible elastic materials for soft robotics, tissue engineering or stretchable electronics with good mechanical properties, tunability, modifiability or healing properties drive technological advance, and yet they are not durable under ambient conditions and do not combine all the attributes in a single platform. We have developed a versatile gelatin-based biogel, which is highly resilient with outstanding elastic characteristics, yet degrades fully when disposed. It self-adheres, is rapidly healable and derived entirely from natural and food-safe constituents. We merge all the favourable attributes in one material that is easy to reproduce and scalable, and has a low-cost production under ambient conditions. This biogel is a step towards durable, life-like soft robotic and electronic systems that are sustainable and closely mimic their natural antetypes.

Robustness of antiadhesion between nanofibers and surfaces covered with nanoripples of varying spatial period.

Since nanofibers have a high surface-to-volume ratio, van der Waals forces render them attracted to virtually any surface. The high ratio provides significant advantages for applications in drug delivery, wound healing, tissue regeneration, and filtration. Cribellate spiders integrate thousands of nanofibers into their capture threads as an adhesive to immobilize their prey. These spiders have antiadhesive nanoripples on the calamistrum, a comb-like structure on their hindmost legs, and are thus an ideal model for investigating how nanofiber adhesion can be reduced. We found that these nanoripples had similar spacing in the cribellate species Uloborus plumipes, Amaurobius similis, and Menneus superciliosus, independent of phylogenetic relation and size. Ripple spacing on other body parts (i.e., cuticle, claws, and spinnerets), however, was less homogeneous. To investigate whether a specific distance between the ripples determines antiadhesion, we fabricated nanorippled foils by nanosecond UV laser processing. We varied the spatial periods of the nanoripples in the range ~203-613 nm. Using two different pulse numbers resulted in ripples of different heights. The antiadhesion was measured for all surfaces, showing that the effect is robust against alterations across the whole range of spatial periods tested. Motivated by these results, we fabricated irregular surface nanoripples with spacing in the range ~130-480 nm, which showed the same antiadhesive behavior. The tested surfaces may be useful in tools for handling nanofibers such as spoolers for single nanofibers, conveyor belts for producing endless nanofiber nonwoven, and cylindrical tools for fabricating tubular nanofiber nonwoven. Engineered fibers such as carbon nanotubes represent a further candidate application area.

Ambient Climate Influences Anti-Adhesion between Biomimetic Structured Foil and Nanofibers.

Due to their uniquely high surface-to-volume ratio, nanofibers are a desired material for various technical applications. However, this surface-to-volume ratio also makes processing difficult as van der Waals forces cause nanofibers to adhere to virtually any surface. The cribellate spider Uloborus plumipes represents a biomimetic paragon for this problem: these spiders integrate thousands of nanofibers into their adhesive capture threads. A comb on their hindmost legs, termed calamistrum, enables the spiders to process the nanofibers without adhering to them. This anti-adhesion is due to a rippled nanotopography on the calamistrum. Via laser-induced periodic surface structures (LIPSS), these nanostructures can be recreated on artificial surfaces, mimicking the non-stickiness of the calamistrum. In order to advance the technical implementation of these biomimetic structured foils, we investigated how climatic conditions influence the anti-adhesive performance of our surfaces. Although anti-adhesion worked well at low and high humidity, technical implementations should nevertheless be air-conditioned to regulate temperature: we observed no pronounced anti-adhesive effect at temperatures above 30 °C. This alteration between anti-adhesion and adhesion could be deployed as a temperature-sensitive switch, allowing to swap between sticking and not sticking to nanofibers. This would make handling even easier.

Spatial Period of Laser-Induced Surface Nanoripples on PET Determines Escherichia coli Repellence.

Bacterial adhesion and biofilm formation on surfaces are associated with persistent microbial contamination, biofouling, and the emergence of resistance, thus, calling for new strategies to impede bacterial surface colonization. Using ns-UV laser treatment (wavelength 248 nm and a pulse duration of 20 ns), laser-induced periodic surface structures (LIPSS) featuring different sub-micrometric periods ranging from ~210 to ~610 nm were processed on commercial poly(ethylene terephthalate) (PET) foils. Bacterial adhesion tests revealed that these nanorippled surfaces exhibit a repellence for E. coli that decisively depends on the spatial periods of the LIPSS with the strongest reduction (~91%) in cell adhesion observed for LIPSS periods of 214 nm. Although chemical and structural analyses indicated a moderate laser-induced surface oxidation, a significant influence on the bacterial adhesion was ruled out. Scanning electron microscopy and additional biofilm studies using a pili-deficient E. coli TG1 strain revealed the role of extracellular appendages in the bacterial repellence observed here.

The external scent efferent system of selected European true bugs (Heteroptera): a biomimetic inspiration for passive, unidirectional fluid transport.

In this work, we present structured capillaries that were inspired by the microstructures of the external scent efferent system as found in different European true bug species (Pentatomidae and Cydnidae). These make use of small, orientated structures in order to facilitate fluid movement towards desired areas where defensive substances are evaporated. Gland channels and microstructures were investigated by means of scanning electron microscopy and abstracted into three-dimensional models. We used these models to create scent channel replicas from different technical substrates (steel and polymers) by means of laser ablation, laser structuring and casting. Video analysis of conducted fluid-flow experiments showed that bug-inspired, artificial scent fluid channels can indeed transport different fluids (water solutions and oils/lubricants) passively in one direction (velocities of up to 1 mm s-1), while halting the fluid movement in the opposite direction. At the end of this contribution, we present a physical theory that explains the observed fluid transport and sets the rules for performance optimization in future work.

Directional, passive liquid transport: the Texas horned lizard as a model for a biomimetic 'liquid diode'.

Moisture-harvesting lizards such as the Texas horned lizard (Iguanidae: Phrynosoma cornutum) live in arid regions. Special skin adaptations enable them to access water sources such as moist sand and dew: their skin is capable of collecting and transporting water directionally by means of a capillary system between the scales. This fluid transport is passive, i.e. requires no external energy, and directs water preferentially towards the lizard's snout. We show that this phenomenon is based on geometric principles, namely on a periodic pattern of interconnected half-open capillary channels that narrow and widen. Following a biomimetic approach, we used these principles to develop a technical prototype design. Building upon the Young-Laplace equation, we derived a theoretical model for the local behaviour of the liquid in such capillaries. We present a global model for the penetration velocity validated by experimental data. Artificial surfaces designed in accordance with this model prevent liquid flow in one direction while sustaining it in the other. Such passive directional liquid transport could lead to process improvements and reduction of resources in many technical applications.