The NeST long ncRNA controls microbial susceptibility and epigenetic activation of the interferon-γ locus.

Long noncoding RNAs (lncRNAs) are increasingly appreciated as regulators of cell-specific gene expression. Here, an enhancer-like lncRNA termed NeST (nettoie Salmonella pas Theiler's [cleanup Salmonella not Theiler's]) is shown to be causal for all phenotypes conferred by murine viral susceptibility locus Tmevp3. This locus was defined by crosses between SJL/J and B10.S mice and contains several candidate genes, including NeST. The SJL/J-derived locus confers higher lncRNA expression, increased interferon-γ (IFN-γ) abundance in activated CD8(+) T cells, increased Theiler's virus persistence, and decreased Salmonella enterica pathogenesis. Transgenic expression of NeST lncRNA alone was sufficient to confer all phenotypes of the SJL/J locus. NeST RNA was found to bind WDR5, a component of the histone H3 lysine 4 methyltransferase complex, and to alter histone 3 methylation at the IFN-γ locus. Thus, this lncRNA regulates epigenetic marking of IFN-γ-encoding chromatin, expression of IFN-γ, and susceptibility to a viral and a bacterial pathogen.

SKAP2 Modular Organization Differently Recognizes SRC Kinases Depending on Their Activation Status and Localization.

Dimerization of SRC kinase adaptor phosphoprotein 2 (SKAP2) induces an increase of binding for most SRC kinases suggesting a fine-tuning with transphosphorylation for kinase activation. This work addresses the molecular basis of SKAP2-mediated SRC kinase regulation through the lens of their interaction capacities. By combining a luciferase complementation assay and extensive site-directed mutagenesis, we demonstrated that SKAP2 interacts with SRC kinases through a modular organization depending both on their phosphorylation-dependent activation and subcellular localization. SKAP2 contains three interacting modules consisting in the dimerization domain, the SRC homology 3 (SH3) domain, and the second interdomain located between the Pleckstrin homology and the SH3 domains. Functionally, the dimerization domain is necessary and sufficient to bind to most activated and myristyl SRC kinases. In contrast, the three modules are necessary to bind SRC kinases at their steady state. The Pleckstrin homology and SH3 domains of SKAP2 as well as tyrosines located in the interdomains modulate these interactions. Analysis of mutants of the SRC kinase family member hematopoietic cell kinase supports this model and shows the role of two residues, Y390 and K7, on its degradation following activation. In this article, we show that a modular architecture of SKAP2 drives its interaction with SRC kinases, with the binding capacity of each module depending on both their localization and phosphorylation state activation. This work opens new perspectives on the molecular mechanisms of SRC kinases activation, which could have significant therapeutic impact.

Configurations of mother-child and father-child attachment relationships as predictors of child language competence: An individual participant data meta-analysis.

An individual participant data meta-analysis was conducted to test pre-registered hypotheses about how the configuration of attachment relationships to mothers and fathers predicts children's language competence. Data from seven studies (published between 1985 and 2014) including 719 children (Mage : 19.84 months; 51% female; 87% White) were included in the linear mixed effects analyses. Mean language competence scores exceeded the population average across children with different attachment configurations. Children with two secure attachment relationships had higher language competence scores compared to those with one or no secure attachment relationships (d = .26). Children with two organized attachment relationships had higher language competence scores compared to those with one organized attachment relationship (d = .23), and this difference was observed in older versus younger children in exploratory analyses. Mother-child and father-child attachment quality did not differentially predict language competence, supporting the comparable importance of attachment to both parents in predicting developmental outcomes.

Maternal distress and parenting during COVID-19: differential effects related to pre-pandemic distress?

BACKGROUND: Distinguishing whether and how pre-existing characteristics impact maternal responses to adversity is difficult: Does prior well-being decrease the likelihood of encountering stressful experiences? Does it protect against adversity's negative effects? We examine whether the interaction between relatively uniformly experienced adversity (due to COVID-19 experience) and individual variation in pre-existing (i.e., pre-pandemic onset) distress predicted mothers' pandemic levels of distress and insensitive caregiving within a country reporting low COVID-19 death rates, and strict nationwide regulations. METHOD: Fifty-one Singaporean mothers and their preschool-aged children provided data across two waves. Pre- pandemic onset maternal distress (i.e., psychological distress, anxiety, and parenting stress) was captured via self-reports and maternal sensitivity was coded from videos. Measures were repeated after the pandemic's onset along with questionnaires concerning perceived COVID-19 adversity (e.g., COVID-19's impact upon stress caring for children, housework, job demands, etc.) and pandemic-related objective experiences (e.g., income, COVID-19 diagnoses, etc.). Regression analyses (SPSS v28) considered pre-pandemic onset maternal distress, COVID-19 stress, and their interaction upon post-pandemic onset maternal distress. Models were re-run with appropriate covariates (e.g., objective experience) when significant findings were observed. To rule out alternative models, follow up analyses (PROCESS Model) considered whether COVID-19 stress mediated pre- and post-pandemic onset associations. Models involving maternal sensitivity followed a similar data analytic plan. RESULTS: Pre-pandemic maternal distress moderated the association between COVID-19 perceived stress and pandemic levels of maternal distress (ß = 0.22, p < 0.01) but not pandemic assessed maternal sensitivity. Perceived COVID-19 stress significantly contributed to post-pandemic onset maternal distress for mothers with pre-pandemic onset distress scores above (ß = 0.30, p = 0.05), but not below (ß = 0.25, p = 0.24), the median. Objective COVID-19 adversity did not account for findings. Post-hoc analyses did not suggest mediation via COVID-19 stress from pre-pandemic to pandemic maternal distress. CONCLUSIONS: Pre-existing risk may interact with subsequent perceptions of adversity to impact well-being. In combination with existing research, this small study suggests prevention programs should focus upon managing concurrent mental health and may highlight the importance of enhanced screening and proactive coping programs for people entering high stress fields and/or phases of life.

A meta-analysis of the distribution of preschool and early childhood attachment as assessed in the strange situation procedure and its modified versions.

This meta-analysis synthesized the distribution of attachment classifications as coded with the Cassidy-Marvin Preschool Attachment Coding System and the Main-Cassidy Six-Year-Old System. These systems have extended scholars' capacity to measure differences in the developing child-parent attachment relationship, and its sequelae, beyond the infancy period; however, the global distribution of the attachment categories in these systems, and the potential factors influencing this distribution, remain unknown. The meta-analysis included 97 samples (N = 8,186 children; 55% boys), mostly drawn from North American or European populations (89%; M = 76% White). Results indicated that the distribution of child-mother attachment was 53.5% secure, 14.0% avoidant, 11.0% ambivalent, and 21.5% disorganized/controlling. Moderator analyses showed that rates of security were lower, and rates of disorganization were higher, in samples of at-risk families, specifically when children were exposed to maltreatment. Variations in the procedure also moderated the distribution. The discussion calls for greater unity around methodological practices.

A meta-analysis on observed paternal and maternal sensitivity.

Two meta-analyses were conducted (N = 10,980 child-father dyads) with 93 studies published between 1983-2020, primarily in North America and Europe, on observed parental sensitivity to children (3-180 months; 48% girls; 14% non-White) in partnered mothers and fathers. The first meta-analysis found higher maternal mean levels of observed sensitivity, with a small effect size (d = -.27). Differences between parents were larger with micro coding and triadic/family assessments. Differences narrowed as a function of publication year and were not significant in European samples. The second meta-analysis identified a moderate correlation between observed maternal and paternal sensitivity (r = .23 after adjusting for probable publication bias). Correlations were larger in Middle Eastern samples and with composite sensitivity scales.

Maternal expressions of positive emotion for children predicts children's respiratory sinus arrhythmia surrounding stress.

The aim of this study is to assess whether positive emotional exchanges (i.e., emotion coregulation) within the mother-child dyad play a protective role in children's physiological response to a distressing task. Specifically, we test whether positive emotion coregulation among mothers and their preschool-aged children is associated with children's respiratory sinus arrhythmia (RSA) at baseline, during, and following a frustration task. One hundred Singaporean mother-child dyads (Mchildage  = 3.5 years) participated in a standardized "Laughing Task" in which positive emotional constructs were measured. Children also participated in a frustration task while RSA was continuously monitored. Hierarchical linear regressions revealed that greater maternal positive emotional responses to children were associated with child RSA at baseline and in recovery from frustration, but not during frustration. These findings have implications for the important role that positive emotion responsivity from mothers may play in children's developing autonomic response systems, and underscore the need for longitudinal work on this topic.

Profiles of Emotion Dysregulation Among University Students Who Self-Injure: Associations with Parent-Child Relationships and Non-Suicidal Self-Injury Characteristics.

More research is needed to understand the different vulnerability profiles of university students who engage in non-suicidal self-injury (NSSI). This study sought to classify university students (n = 479; 83.8% female) aged 17-25 years (M = 18.77; SD = 1.42) who had engaged in NSSI within the past year into latent profiles based on their self-perceived difficulties in regulating both positive and negative emotions. Independent samples of students who had a past history of NSSI but had not self-injured within the previous year (n = 439; 82.9% females; Mage = 19.03, SD = 1.62) and who had no history of NSSI (n = 1551; 69.9% females; Mage = 19.02, SD = 1.55) were recruited for comparison purposes. Latent cluster analyses revealed three emotion regulation profiles within the NSSI sample-the Average Difficulties (47.4%), Dysregulated (33.0%), and Low Difficulties (19.6%) profiles-each of which differed meaningfully from both comparison samples on mean emotion regulation difficulties. Students across profiles also differed in their self-reported experiences with parents, particularly with fathers (pressure, antipathy, unresolved attachment, psychological control), and in the extent to which they felt alienated from parents. Lastly, students across profiles differed in the frequency, methods, functions, and addictive properties of their NSSI. Findings highlight that university students who self-injure experience distinct patterns of difficulties with emotion regulation, which are associated with variation in parent-child relational risk factors and NSSI outcomes.

Configurations of mother-child and father-child attachment as predictors of internalizing and externalizing behavioral problems: An individual participant data (IPD) meta-analysis.

An unsettled question in attachment theory and research is the extent to which children's attachment patterns with mothers and fathers jointly predict developmental outcomes. In this study, we used individual participant data (IPD) meta-analysis to assess whether early attachment networks with mothers and fathers are associated with children's internalizing and externalizing behavioral problems. Following a pre-registered protocol, data from 9 studies and 1,097 children (mean age: 28.67 months) with attachment classifications to both mothers and fathers were included in analyses. We used a linear mixed effects analysis to assess differences in children's internalizing and externalizing behavioral problems as assessed via the average of both maternal and paternal reports based on whether children had two, one, or no insecure (or disorganized) attachments. Results indicated that children with an insecure attachment relationship with one or both parents were at higher risk for elevated internalizing behavioral problems compared with children who were securely attached to both parents. Children whose attachment relationships with both parents were classified as disorganized had more externalizing behavioral problems compared to children with either one or no disorganized attachment relationship with their parents. Across attachment classification networks and behavioral problems, findings suggest (a) an increased vulnerability to behavioral problems when children have insecure or disorganized attachment to both parents, and (b) that mother-child and father-child attachment relationships may not differ in the roles they play in children's development of internalizing and externalizing behavioral problems.

Preschool father-child attachment and its relation to self-reported child socioemotional adaptation in middle childhood.

Although considerable research has sought to establish the influence of parent-child attachment on child socioemotional adaptation, it has primarily focused on mother-child dyads and external reports of adaptation. The current study investigates the longitudinal associations between both preschool mother-child and father-child attachment and self-reported socioemotional adaptation in middle childhood. Eighty-three children (47 girls) participated in separate lab visits with each parent at both Time 1 (3-5 years old) and Time 2 (7-11 years old). Results revealed that father-child attachment assessed with the MacArthur Preschool Attachment Coding System was uniquely related to child self-esteem, such that insecure children had significantly lower self-esteem scores than what was expected by chance. Children insecurely attached to their father also reported significantly higher externalizing problems than secure children, but this relationship was only significant if the child was also insecurely attached to their mother.

Validation of the Preschool Attachment Rating Scales with child-mother and child-father dyads.

Growing evidence points to the theoretical and statistical advantages of continuous (rather than categorical) assessments of child-caregiver attachment. The Preschool Attachment Rating Scales (PARS) is a continuous coding system to assess preschool attachment that is complementary to the categorical MacArthur Preschool Attachment Coding System (PACS). The current study aims to evaluate the reliability and validity of the PARS to measure both child-mother and child-father attachment during the preschool period. Participants included 144 preschool-aged children (M = 46.89 months, SD = 8.77; 83 girls) and their parents. Results support the reliability and validity of the PARS: good inter-rater reliability, expected associations between scales, convergence with the PACS, and association with parental sensitivity and child externalizing problems. These findings support the application of continuous assessments of child-caregiver attachment in the preschool years. They also align with previous work on child-mother attachment, and present avenues for future research on child-father attachment.

Invalidating Caregiving Environments, Specific Emotion Regulation Deficits, and Non-suicidal Self-injury.

This study examined the indirect effects of distinct aspects of invalidating caregiving environments (i.e., paternal maltreatment, maternal maltreatment, and perceived alienation) on non-suicidal self-injury (NSSI) via six specific emotion regulation difficulties. We hypothesized that specific emotion regulation deficits would mediate associations between invalidating environments and NSSI. Participants included 114 young adults (57 self-injurers; 57 age- and sex-matched comparison participants) aged 17-25 years. Three parallel mediation models tested hypotheses. Results showed that maternal maltreatment, paternal maltreatment, and perceived alienation indirectly predicted NSSI through poor emotional clarity. Maternal maltreatment uniquely predicted NSSI through limited access to regulation strategies. Lastly, maternal maltreatment and perceived alienation were both linked to greater difficulties engaging in goal-directed behavior during emotional upsets; however, contrary to hypotheses, this particular deficit was associated with decreased odds of engaging in NSSI. Findings illustrate how different aspects of invalidating environments and specific emotion regulation deficits may be implicated in NSSI engagement.

Fear of Failure Mediates the Relation Between Parental Psychological Control and Academic Outcomes: A Latent Mediated-Moderation Model of Parents' and Children's Genders.

Given the negative relations between parental psychological control and various aspects of emerging adults' social and emotional adaptation, there is a need to determine whether similar relations exist for emerging adults' academic adjustment. The current study tested an integrative model using an interactionist approach of dyadic gender composition to test whether maternal and paternal psychological control are unique and interactive predictors of four different indicators of academic adjustment (i.e., academic achievement, satisfaction of academic achievement, academic goal progress, and school satisfaction) for male and female emerging adults, using fear of failure as a mediator. The sample comprised 1792 undergraduate students aged 17-25 years old (74.2% identified as females). The results showed that maternal and paternal psychological control interacted to predict students' fear of failure, and that fear of failure negatively predicted all indicators of academic adjustment. The results revealed small indirect mediation effects. For females, fear of failure mediated the relation between the interaction of psychological control and satisfaction of academic achievement. For males, fear of failure mediated the relation between the interaction of psychological control and academic goal progress. The results show that parents continue to play an important role in their children's lives during emerging adulthood, and provide insight on the mechanisms underlying such parental influence.

The contributions of child-mother attachment, maternal parenting stress, and military status to the prediction of child behavior problems.

Studies show that children with a military parent are at heightened risk of the development of behavior problems. However, there is limited work examining how other factors experienced by military families may also influence behavior problems. In the current study, we recruited three types of Canadian families with a preschooler: families with a deployed military member, families with a nondeployed military member, and nonmilitary families. We examined whether the nonmilitary parent's (in all cases the mother) parenting stress and attachment relationship with the child are associated with behavior problems, and whether deployment status further contributes to the prediction. Child-mother dyads participated in an observed attachment assessment, and mothers reported on their stress levels and their child's behavior. Results showed that both child attachment insecurity and parenting stress were associated with elevated levels of internalizing problems; however, only parenting stress was associated with conduct problems. Military deployment predicted higher levels of internalizing and conduct problems beyond the contributions of attachment and stress. Furthermore, having a father in the military (whether deployed or not) also contributed to internalizing problems. These findings shed light on how the military lifestyle impacts early childhood mental health through the complex interplay between various parts of their environment.

Los estudios muestran que los niños con un progenitor en las fuerzas armadas se encuentran a un más alto riesgo para desarrollar problemas de comportamiento. Sin embargo, es limitado el trabajo que examina cómo otros factores experimentados por familias con miembros en las fuerzas armadas pudieran también influir en los problemas de comportamiento. En el presente estudio, reclutamos tres tipos de familias con un niño en edad prescolar: familias en las que un miembro había sido enviado al servicio militar activo; familias con un miembro que no había sido enviado al servicio militar activo, y familias sin un miembro en las fuerzas armadas. Examinamos si el estrés de la crianza del progenitor no miembro de las fuerzas armadas (en todos los casos la madre) y la relación de afectividad con el niño están asociadas con los problemas de comportamiento, y si la condición de haber sido enviado al servicio militar activo contribuye aún más a esta predicción. Las díadas madre-niño participaron en una evaluación observada de la afectividad, y las madres reportaron acerca de sus niveles de estrés y el comportamiento de sus niños. Los resultados mostraron que tanto la inseguridad de la afectividad en el niño como el estrés de la crianza estaban asociados con niveles elevados de problemas de internalización, sin embargo, sólo el estrés de la crianza estaba asociado con problemas de comportamiento. El hecho de ser enviado al servicio militar activo predijo más altos niveles de problemas de internalización y de comportamiento más allá de lo que contribuye la afectividad y el estrés. Es más, el tener un papá en las fuerzas armadas (ya sea que haya sido enviado al servicio militar activo o no), también contribuye a problemas de internalización. Estos resultados arrojan luz sobre cómo el estilo de vida de estas familias con un miembro de las fuerzas armadas impacta la salud mental en la temprana niñez a través de la compleja interacción entre varias partes de su entorno. Palabras claves: fuerzas armadas, asignación al servicio militar activo, afectividad, problemas de comportamiento, estrés de crianza.

Les études montrent que les enfants dont l'un des parents est dans les forces armées sont à un risque bien plus élevé de développer des problèmes de comportement. Cependant peu de recherches examinent comment d'autres facteurs dont font l'expérience les familles de militaires peuvent également influencer les problèmes de comportement. Dans cette étude nous avons recruté trois types de familles ayant un d'enfant d'âge préscolaire (avant la grande maternelle): des familles dont l'un des membres est un militaire déployé, des familles avec un membre militaire n'étant pas déployé, et des familles non-militaires. Nous avons examiné si le stress de parentage du parent non-militaire (dans tous les cas la mère) et la relation d'attachement avec l'enfant étaient liés aux problèmes de comportement, et si le statut de déploiement contribue plus à la prédiction. Les dyades enfant-mère ont participé à une évaluation d'attachement observée, et les mères ont fait état de leurs niveaux de stress et du comportement de leurs enfants. Les résultats ont montré qu'à la fois l'insécurité de l'attachement de l'enfant et le stress de parentage était lié à des niveaux élevés de problèmes d'internalisation. Cependant, seul le stress de parentage était lié à des problèmes de comportement. Le déploiement militaire prédisait des niveaux d'internalisation plus élevés et des problèmes de conduite au delà des contributions à l'attachement et au stress. De plus, le fait d'avoir un père dans les forces armées (qu'il soit déployé ou non) contribuait aussi aux problèmes d'internalisation. Ces résultats mettent en lumière la manière dont le style de vie militaire impacte la santé mentale de la petite enfance au travers d'une interaction complexe entre les différentes parties de leur environnement. Mots clés: militaire, déploiement, attachement, problèmes de comportement, stress de parentage.

Detecting multi-way epistasis in family-based association studies.

The era of genome-wide association studies (GWAS) has led to the discovery of numerous genetic variants associated with disease. Better understanding of whether these or other variants interact leading to differential risk compared with individual marker effects will increase our understanding of the genetic architecture of disease, which may be investigated using the family-based study design. We present M-TDT (the multi-locus transmission disequilibrium test), a tool for detecting family-based multi-locus multi-allelic effects for qualitative or quantitative traits, extended from the original transmission disequilibrium test (TDT). Tests to handle the comparison between additive and epistatic models, lack of independence between markers and multiple offspring are described. Performance of M-TDT is compared with a multifactor dimensionality reduction (MDR) approach designed for investigating families in the hypothesis-free genome-wide setting (the multifactor dimensionality reduction pedigree disequilibrium test, MDR-PDT). Other methods derived from the TDT or MDR to investigate genetic interaction in the family-based design are also discussed. The case of three independent biallelic loci is illustrated using simulations for one- to three-locus alternative hypotheses. M-TDT identified joint-locus effects and distinguished effectively between additive and epistatic models. We showed a practical example of M-TDT based on three genes already known to be implicated in malaria susceptibility. Our findings demonstrate the value of M-TDT in a hypothesis-driven context to test for multi-way epistasis underlying common disease etiology, whereas MDR-PDT-based methods are more appropriate in a hypothesis-free genome-wide setting.

Greater caregiving risk, better infant memory performance?

Poor early life care often relates to cognitive difficulties. However, newer work suggests that in early-life, adversity may associate with enhanced or accelerated neurodevelopment. We examine associations between postnatal caregiving risks (i.e., higher self-reported postnatal-anxiety and lower observed maternal sensitivity) and infant relational memory (i.e., via deferred imitation and relational binding). Using subsamples of 67-181 infants (aged 433-477 post-conceptual days, or roughly five to seven months since birth) taking part in the GUSTO study, we found such postnatal caregiving risk significantly predictive of "better" performance on a relational binding task following a brief delay, after Bonferroni adjustments. Subsequent analyses suggest that the association between memory and these risks may specifically be apparent among infants spending at least 50% of their waking hours in the presence of their mothers. Our findings echo neuroimaging research concerning similar risk exposure and larger infant hippocampal volume, and likewise underscore the importance of considering developmental context in understanding early life experience. With this in mind, these findings caution against the use of cognitive outcomes as indices of experienced risk.

Mother-Child Attachment From Infancy to the Preschool Years: Predicting Security and Stability.

Relations between maternal mind-mindedness (appropriate and nonattuned mind-related comments), children's age-2 perspective-taking abilities, and attachment security at 44 (n = 165) and 51 (n = 128) months were investigated. Nonattuned comments predicted insecure preschool attachment, via insecure 15-month attachment security (44-month attachment) and poorer age-2 perspective-taking abilities (51-month attachment). With regard to attachment stability, higher perspective-taking abilities distinguished the stable secure groups from (a) the stable insecure groups and (b) children who changed from secure to insecure (at trend level). These effects were independent of child gender, stressful life events, and socioeconomic status (SES). The contribution of these findings to our understanding of stability and change in attachment security from infancy to the preschool years is discussed.

Childhood attachment and behavioral inhibition: Predicting intolerance of uncertainty in adulthood.

Intolerance of uncertainty (IU), the tendency to react negatively to uncertain situations, has been identified as an important cognitive component of anxiety disorders, yet little is known about its etiology. Links to temperament, particularly behavioral inhibition (BI), and insecure attachment have been proposed in the development of IU, but no prospective empirical investigation has been performed thus far. In the current study, attachment to caregiver and BI of 60 children were assessed at age 6, using observational measures. Mother's anxiety symptoms were assessed when participants were 14 years old. IU was reported by participants when they were 21 years old, as was neuroticism. Two types of insecure attachment (ambivalent and disorganized-controlling) and BI were positively related to IU over a 15-year span, even after controlling for participants' neuroticism and maternal anxiety. Attachment and BI had no significant interacting effect on the development of IU. Maternal anxiety was positively related to child BI and insecure attachment, but not IU. This study is the first to provide empirical support for a link between ambivalent and disorganized-controlling attachment and BI in preschool children to the development of IU in adulthood. Results have etiological and preventative implications not only for anxiety disorders but also for all disorders related to IU.

The role of ethnicity and socioeconomic status in Southeast Asian mothers' parenting sensitivity.

Past research indicates that socioeconomic status (SES) accounts for differences in sensitivity across ethnic groups. However, comparatively little work has been conducted in Asia, with none examining whether ethnicity moderates the relation between SES and sensitivity. We assessed parenting behavior in 293 Singaporean citizen mothers of 6-month olds (153 Chinese, 108 Malay, 32 Indian) via the Maternal Behavioral Q-Sort for video interactions. When entered into the same model, SES (F(1,288) = 17.777, p < .001), but not ethnicity, predicted maternal sensitivity (F(2,288) = .542, p = .582). However, this positive relation between SES and sensitivity was marginally moderated by ethnicity. SES significantly positively predicted sensitivity in Chinese, but not Malay dyads. Within Indian dyads, SES marginally positively predicted sensitivity only when permanent residents were included in analyses. We discuss the importance of culture on perceived SES-associated stress. However, because few university-educated Malays participated, we also consider whether university education, specifically, positively influences sensitivity.

Middle Childhood Attachment Strategies: validation of an observational measure.

The purpose of this study was to assess behavioral manifestations of attachment in middle childhood, and to evaluate their relations with key theoretical correlates. The sample consisted of 87 children (aged 10-12 years) and their mothers. Dyads participated in an 8-min videotaped discussion of a conflict in their relationships, later scored with the Middle Childhood Attachment Strategies Coding System (MCAS) for key features of all child attachment patterns described in previous literature (secure, ambivalent, avoidant, disorganized-disoriented, caregiving/role-confused, hostile/punitive). To assess validity, relations among MCAS dimensions and other measures of attachment, parenting, and psychological adjustment were evaluated. Results provide preliminary evidence for the psychometric properties of the MCAS in that its behaviorally assessed patterns were associated with theoretically relevant constructs, including maternal warmth/acceptance and psychological control, and children's social competence, depression, and behavioral problems. The MCAS opens new grounds for expanding our understanding of attachment and its outcomes in middle childhood.