RESUMO
We describe a simple and inexpensive method of fabricating single cell magnetic traps within a polydimethylsiloxane (PDMS) device. These traps were developed as part of an automated system that captures individual yeast cells in a microfluidic device and analyzes each cell as it buds. To make the traps, PdCl(2) catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B alloy is electrolessly deposited onto the catalyst at a moderate temperature. We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel.
Assuntos
Técnicas Analíticas Microfluídicas , Microscopia de Força Atômica , Saccharomycetales/ultraestrutura , Ligas/química , Catálise , Células Imobilizadas/ultraestrutura , Dimetilpolisiloxanos/química , Magnetismo , Paládio/química , Saccharomycetales/crescimento & desenvolvimento , Silicones/químicaAssuntos
Inibidores da Angiogênese/uso terapêutico , Anticorpos Monoclonais/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Inibidores da Angiogênese/economia , Anticorpos Monoclonais/economia , Anticorpos Monoclonais Humanizados , Bevacizumab , Feminino , Humanos , Opinião Pública , Ciência , Resultado do Tratamento , Estados Unidos , United States Food and Drug AdministrationRESUMO
Evidence indicates that, despite some critical successes, current conservation approaches are not slowing the overall rate of biodiversity loss. The field of synthetic biology, which is capable of altering natural genomes with extremely precise editing, might offer the potential to resolve some intractable conservation problems (e.g., invasive species or pathogens). However, it is our opinion that there has been insufficient engagement by the conservation community with practitioners of synthetic biology. We contend that rapid, large-scale engagement of these two communities is urgently needed to avoid unintended and deleterious ecological consequences. To this point we describe case studies where synthetic biology is currently being applied to conservation, and we highlight the benefits to conservation biologists from engaging with this emerging technology.
Assuntos
Biodiversidade , Conservação dos Recursos Naturais , Biologia Sintética , Ecologia , Espécies IntroduzidasAssuntos
Neoplasias/tratamento farmacológico , Pesquisa/tendências , Inibidores da Angiogênese/uso terapêutico , Anticorpos Monoclonais/uso terapêutico , Anticorpos Monoclonais Humanizados , Antineoplásicos/uso terapêutico , Bevacizumab , Cloridrato de Erlotinib , Feminino , Humanos , Ácidos Hidroxâmicos/uso terapêutico , Masculino , Inibidores de Proteínas Quinases/uso terapêutico , Quinazolinas/uso terapêutico , Sociedades Médicas , Estados Unidos , VorinostatRESUMO
Thin polydimethylsiloxane (PDMS) films are frequently used in "lab on a chip" devices as flexible membranes. The common solvent used to dilute the PDMS for thin films is hexane, but hexane can swell the underlying PDMS substrate. A better solvent would be one that dissolves uncured PDMS but doesn't swell the underlying substrate. Here, we present protocols and spin curves for two alternatives to hexane dilution: longer spin times and dilution in tert-butyl alcohol. The thickness of the PDMS membranes under different spin speeds, spin times, and PDMS concentrations was measured using an optical profilometer. The use of tert-butyl alcohol to spin thin PDMS films does not swell the underlying PDMS substrate, and we have used these films to construct multilayer PDMS devices.