Protective effects of fecal microbiota transplantation in sepsis are independent of the modulation of the intestinal flora.

OBJECTIVE: The aim of this study was to investigate the protective effects of probiotics and fecal transplantation on inflammatory and oxidative parameters in the intestines of two rat models of sepsis. METHODS: Rats were treated with prebiotics, probiotics, or symbiotics and exposed to lipopolysaccharide (LPS) or zymosan after 15 d to induce endotoxemia. Oxidative damage and inflammation were analyzed, and histologic examination of the intestinal tissue was performed. Fecal microbiota transplantation (FMT) was carried out in LPS- and zymosan-induced rat models of sepsis. RESULTS: Supplementation with symbiotics for 15 d effectively reduced the inflammatory parameters compared with supplementation for 7 d. Probiotics, prebiotics, and symbiotics exerted different effects on the evaluated parameters. In general, Lactobacillus rhamnosus and L. casei exerted better local protective effects. Evaluation of the role of the intestinal microbiota through FMT revealed its protective effects irrespective of the previous treatment with probiotics. CONCLUSION: Probiotic strains significantly differ among themselves and exert different effects on the host's health. Symbiotics and FMT could offer additional immunomodulatory benefits to drug therapy, thus serving as a new therapeutic alternative in pediatric patients with sepsis.

Early life experience contributes to the developmental programming of depressive-like behaviour, neuroinflammation and oxidative stress.

This study used an animal model of depression induced by maternal care deprivation (MCD) to investigate whether depressive behaviour, neuroinflammation and oxidative stress were underlying factors in developmental programming after early life stress. At postnatal days (PND) 20, 30, 40, and 60, individual subsets of animals were evaluated in behavioural tests and then euthanized to assess cytokine levels and oxidative stress parameters in the prefrontal cortex (PFC), hippocampus and serum. The results showed that MCD did not induce behavioural changes at PND 30 and 40. However, at PND 20 and 60, the rats displayed a depressive-like behaviour in the forced swimming test, without changes in locomotor spontaneous activity. In the brain and serum, the levels of pro-inflammatory cytokines (interleukin-1ß (IL-1ß), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α)) were increased, and the anti-inflammatory cytokine (interleukin-10) level was reduced throughout developmental programming (PND 20, 30, 40 and 60). Protein carbonyl levels increased in the brain at PND 30, 40 and 60. Superoxide dismutase (SOD) activity was decreased during all developmental programming phases evaluated in the brain. Catalase (CAT) activity was decreased at PND 20, 40 and 60 in the brain. Our results revealed that "critical episodes" in early life stressful events are able to induce behavioural alterations that persist into adulthood and can stimulate inflammation and oxidative damage in both central and peripheral systems, which are required for distinct patterns of resilience against psychiatric disorders later in life.

'Hot' Earth in the mass media: the reliability of news reports on global warming.

Research into the reliability of news reports on 'global warming' published by the UOL media group, Folha.com and Folha de S. Paulo reveals a tendency for positions to be polarized between complete agreement with the assertion that the causes are entirely anthropogenic (the dominant position) and complete denial. The sample comprised 676 news items from more than 3,000 published on the topic between October 2007 and October 2008. The study tested the hypothesis that the news output of the three media outlets is dominated by the positions of the Intergovernmental Panel on Climate Change. In absolute terms, the panel is the most frequently cited source, since just seven news items comprised exceptions to the 'consensus.' These contrary opinions made up 1.03% of the sample.

A Terra 'quente' na imprensa: confiabilidade de notícias sobre aquecimento global / 'Hot' Earth in the mass media: the reliability of news reports on global warming

Pesquisa sobre confiabilidade de notícias a respeito do 'aquecimento global', em veículos do grupo de comunicação UOL, Folha.com e Folha de S. Paulo, revelou certa polaridade de posições entre a concordância plena de que as causas sejam exclusivamente antrópicas (posição predominante) e sua completa negação. A amostra compreende 676 notícias, entre mais de três mil relacionadas ao tema, entre outubro de 2007 e outubro de 2008. Avaliou-se a hipótese de ser o noticiário dos três meios de comunicação dominado pelas posições do Painel Intergovernamental de Mudança Climática. Em termos absolutos, o Painel é a fonte mais referenciada, pois apenas sete notícias constituem exceções ao 'consenso'. Tais opiniões contrárias perfazem 1,03% da amostra.

Research into the reliability of news reports on 'global warming' published by the UOL media group, Folha.com and Folha de S. Paulo reveals a tendency for positions to be polarized between complete agreement with the assertion that the causes are entirely anthropogenic (the dominant position) and complete denial. The sample comprised 676 news items from more than 3,000 published on the topic between October 2007 and October 2008. The study tested the hypothesis that the news output of the three media outlets is dominated by the positions of the Intergovernmental Panel on Climate Change. In absolute terms, the panel is the most frequently cited source, since just seven news items comprised exceptions to the 'consensus.' These contrary opinions made up 1.03% of the sample.