[Clinical phenotype and genetic features of 16p11.2 microdeletion-related epilepsy in children]. / 16p11.2å¾®ç¼ºå¤±ç›¸å ³å„¿ç«¥ç™«ç—«çš„ä¸´åºŠè¡¨åž‹ä¸Žé—ä¼ å­¦ç‰¹å¾åˆ†æž.

OBJECTIVES: To study the clinical phenotype and genetic features of 16p11.2 microdeletion-related epilepsy in children. METHODS: The medical data of 200 children with epilepsy who underwent a genetic analysis of epilepsy by the whole exon sequencing technology were collected retrospectively, of whom 9 children with epilepsy had 16p11.2 microdeletion. The clinical phenotype and genetic features of the 9 children with 16p11.2 microdeletion were analyzed. RESULTS: The detection rate of 16p11.2 microdeletion was 4.5% (9/200). The 9 children with 16p11.2 microdeletion were 3-10 months old. They experienced focal motor seizures with consciousness disturbance, and some of the seizures developed into generalized tonic-clonic seizures. The interictal electroencephalogram showed focal or multifocal epileptiform discharge, and all 9 children responded well to antiepileptic drugs. The 9 children had a 16p11.2 deletion fragment size of 398-906 kb, and the number of deleted genes was 23-33 which were all pathogenic mutations. The mutation was of maternal origin in 2 children, of paternal origin in 1 child, and de novo in the other children. CONCLUSIONS: 16p11.2 microdeletion can be detected in some children with epilepsy. Most of the 16p11.2 microdeletion is de novo mutation and large gene fragment deletion. The onset of 16p11.2 microdeletion-related epilepsy in children is mostly within 1 year of life, and the epilepsy is drug-responsive.

Estimation of soil organic matter content in Yinchuan Plain based on fractional derivative combined with spectral indices.

Soil organic matter (SOM) is a crucial indicator of soil fertility. Field hyperspectral reflectance and laboratory SOM data of soil samples from the Yinchuan Plain were used to explore the performance of models based on fractional derivative combined with different spectral indices. Following reciprocal and logarithmic transformation, the reflectance data were processed using fractional derivative from 0 to 2 orders (interval 0.20). Then, the difference index (DI), ratio index (RI), brightness index (BI), normalized difference index (NDI), renormalized difference index (RDI), and generalized difference index (GDI) were constructed. The two-dimensional correlation between the six indices and SOM content were analyzed. The optimal spectral indices were selected to establish SOM estimation models with principal component regression (PCR), partial least square regression (PLSR), back propagation neural network (BPNN), support vector machine (SVM), and geographically weighted regression (GWR). Results showed that the maximum absolute correlation coefficient (MACC) values between DI, RI, NDI, BI, GDI, RDI, and SOM contents increased firstly and then decreased, with the highest values observed at 1.0, 0.6, 1.4, and 1.6 orders. The 0.2-2.0 order RDI under fractional derivative variation could be used for subsequent model construction, in which the optimal combinations of bands for MACC values were mainly concentrated at 400-600 nm and 1300-1700 nm. Among the different models based on the single spectral index RDI, the model based on SVM achieved the highest estimation accuracy, whose modeling determination coefficient, verification determination coefficient and relative percentage difference reached 0.86, 0.87 and 2.32. Our results would provide a scientific reference for quick and accurate SOM assessment and mapping in areas with relatively low SOM content.

Estimation of soil water and organic matter content in medium and low yield fields of Ningxia Yellow River Irrigation area based on hyperspectral information.

Accurately obtaining soil water and organic matter content is of great significance for improving soil qua-lity in croplands with medium to low yield. We explored the estimation effect of fractional order differentiation (FOD) combined with different spectral indices on soil water and organic matter content in medium and low yield croplands of Ningxia Yellow River Irrigation Area. After root mean square transformation of field measured hyperspectral reflectance, we used 0-2 FOD (with a step length of 0.25) to construct difference index (DI), ratio index (RI), product index (PI), sum index (SI), generalized difference index (GDI), and nitrogen planar domain index (NPDI) and to select the optimal spectral index based on the correlation coefficients between six spectral indices with soil water and organic matter contents. We constructed a model for estimating soil water and organic matter content based on partial least squares regression (PLSR) and support vector machine (SVM). The results showed that the correlation between soil water and organic matter content and spectral information was effectively improved after FOD transformation compared with the original spectrum, with maximum increases of 0.1785 and 0.1713, respectively. The soil water content sensitive bands were mainly in the range of 400-630 and 1350-1940 nm, while the sensitive bands of organic matter content were mainly at 460-850, 1530-1910, and 2060-2310 nm. The accuracy of SVM model was significantly higher than that of PLSR, and the soil water content estimation model based on 1.75-order NPDI-SVM reached the highest precision, with a validation determination coefficient (Rp2) of 0.970, root mean square error (RMSE) of 1.615, and relative percent deviation (RPD) of 4.211. The organic matter content estimation model based on 0.5 order DI-SVM had the best performance, with Rp2, RMSE and RPD of 0.983, 0.701 and 5.307, respectively. Our results could provide data and technological support for soil water and nutrient monitoring, quality improvement, and graphics creating in similar area with medium to low yield fields.

Hyperspectral inversion of soil water and salt information based on fractional order derivative technology.

Accurate and efficient acquisition of soil water and salt information is a prerequisite for the improvement and sustainable utilization of saline lands. With the ground field hyperspectral reflectance and the measured soil water-salt content as data sources, we used the fractional order differentiation (FOD) technique to process hyperspectral data (with a step length of 0.25). The optimal FOD order was explored at the correlation level of spectral data and soil water-salt information. We constructed two-dimensional spectral index, support vector machine regression (SVR) and geographically weighted regression (GWR). The inverse model of soil water-salt content was finally evaluated. The results showed that FOD technique could reduce the hyperspectral noise and explore the potential spectral information to a certain extent, improve the correlation between spectrum and characteristics, with the highest correlation coefficients of 0.98, 1.35 and 0.33. The combination of characteristic bands screened by FOD and two-dimensional spectral index were more sensitive to characteristics than one-dimensional bands, with the optimal responses of order 1.5, 1.0 and 0.75. The optimal combinations of bands for maximum absolute correction coefficient of SMC were 570, 1000, 1010, 1020, 1330 and 2140 nm, pH were 550, 1000, 1380 and 2180 nm, and salt content were 600, 990, 1600 and 1710 nm, respectively. Compared with the original spectral reflectance, the validation coefficients of determination (Rp2) of the optimal order estimation models for SMC, pH, and salinity were improved by 1.87, 0.94 and 0.56, respectively. The overall GWR accuracy in the proposed model was better than SVR, where the GWR optimal order estimation models Rp2 were 0.866, 0.904 and 0.647, and the relative per-centage difference were 3.54, 4.25 and 1.86, respectively. The overall spatial distribution of soil water and salt content in the study area was characterized by low in the west and high in the east, with more serious soil alkalinization problems in the northwest and less severe in the northeast. The results would provide scientific basis for the hyperspectral inversion of soil water and salt in the Yellow River Irrigation Area and a new strategy for the implementation and management of precision agriculture in saline soil areas.

[Effects of different spectra types on the accuracy and correction of soil salt content inversion in Yinchuan Plain, China]. / ä¸åŒå ‰è°±ç±»åž‹å¯¹é“¶å·å¹³åŽŸåœŸå£¤å«ç›é‡åæ¼”ç²¾åº¦çš„å½±å“ä¸Žæ ¡æ­£.

Soil salinization is one of key drivers for the degradation of soil quality and yield in arable land. To accurately and quickly evaluate soil salt content in Yinchuan Plain, field and indoor hyperspectral data were processed with first order differential (FDR) transformation, then the feature bands were identified by stepwise regression (SR). Partial least squares regression (PLSR) and support vector machines (SVM) were used to build models, which were verified to figure out the optimal hyperspectral type for the study area. Moreover, segmented and global corrections were performed to process poor hyperspectral, aiming to improve the accuracy of soil salt content inversion. The results showed that the accuracy of soil salt content inversion model based on field hyperspectral data was 58.9% higher than that of the indoor hyperspectral data. The accuracy of the inversion was improved through the segmented and global correction of the indoor hyperspectral. We found that the segmented correction is more accurate for the PLSR model (Rc2=0.790, Rp2=0.633, RPD=1.64) and the global correction is more accurate for the SVM model (Rc2=0.927, Rp2=0.947, RPD=3.87). The SVM models' inversion accuracy was higher than that of PLSR, with the field hyperspectral model fitted the best, followed by the indoor hyperspectral processed with the global correction and the indoor hyperspectral processed with the segmented correction, while the indoor hyperspectral the worst. Our results suggest that field hyperspectral data could contribute to the quantitative inversion of soil salt content in Yinchuan Plain. The corrected indoor hyperspectral could significantly enhance the inversion accuracy of soil salt content, which could guarantee food security and ecological quality development.

Estimation of soil Na + content based on measured hyperspectral and Sentinel-2B data in northern Ningxia, China.

To explore the ability of different sensors to estimate soil Na+ content, we got the mea-sured soil spectra and Sentinel-2B image spectra of the typical soil samples from the northern area of Ningxia. We filtered the sensitive parameters from the spectra data by means of stepwise regression (SR) and principal component regression analysis (PCA). We established the models to estimate soil Na+ content based on the measured spectra and image data using partial least square regression (PLSR), support vector machine (SVM) and back propagation neural network model (BPNN). The results showed that, except for Band9, there was significant correlation between the resampling data and the image data. The estimation accuracy of models based on SR-screening was generally higher than the PCA (excluding SVM model). The PCA-SVM model was the best image estimation model for soil Na+ content, with a prediction accuracy of 0.792. The SR-BPNN model was the best measured estimation model, with a prediction accuracy of 0.908. The estimating accuracy of the SR-PLSR image-spectra-based model increased from 0.481 to 0.798 after calibrated by the resampled measured spectrum model, which effectively enhanced the accuracy in estimating the soil Na+ content at large scale. We successfully made the spatial transformation of soil Na+ content from point to surface. Our results provided a scientific reference for Sentinel-2B image to monitor Na+ content in salinized soil.

[Distribution Characteristics of Antibiotics and Antibiotic Resistance Genes in Manure and Surrounding Soil of Cattle Farms in Ningxia].

Livestock breeding intensively uses veterinary antibiotics in concentrated feeding operations to improve growth and control disease. Consequently, livestock and poultry manure is an important repository of antibiotics and antibiotic resistance genes (ARGs). To understanding the distribution of antibiotics and ARGs in manure and surrounding soils of cattle farms in Ningxia, cattle manure from five breeding periods (lactation, calving, growing, pre-fattening, and post-fattening periods) and comparative soil samples were collected from the largest beef-breeding area in Ningxia. The compositions of ARGs in the samples were analyzed by UPLC-MS/MS and HT-qPCR. The results showed that:① Tetracycline, quinolone, and sulfonamide were the dominant antibiotics in cattle manure. The content of antibiotics in the manure varied greatly between different breeding periods. High amounts of antibiotics were detected during the pre-fattening and lactation periods, and the lowest amounts were detected during the calving period. ② Quinolone and tetracycline were the dominant antibiotics in the soils, and the detection rate and content of quinolone were highest. The cattle farms did not affect the distribution of antibiotics in the surrounding soils. The content of quinolone and tetracycline in the soils with cattle manure application were significantly higher than control and surrounding soils. ③ We detected 79-142 ARGs in cattle manure, with aminoglycosides the most common form. The number and relative abundance of ARGs were highest during the growing period and lowest during the calving period. The dominant ARGs were tetQ, ermF, and tetO-01 across all the breeding periods. ④ There were 35-79 ARGs in the tested soils, and multidrug and aminoglycoside ARGs were dominant. The cattle farms did not affect the number and relative abundance of ARGs in the surrounding soils; however, manure application significantly affected the number and relative abundance of ARGs. ⑤ Sulfonamide and chloramphenicol ARGs are at risk of mobilization and horizontal transport. A correlation analysis showed that the relative abundances of aminoglycoside and tetracycline in cattle manure were significantly positively correlated with their contents. ⑥ Aminoglycoside, sulfonamide, chloramphenicol, and mobile genetic elements (MGEs) in manure were significantly or extremely significantly positively correlated with aminoglycosides and sulfonamides in the soils, whereas macrolides were negatively correlated with vancomycin. These results provide baseline data to inform controls on the variety and dosages of feed and veterinary drugs in cattle farms and the application of organic fertilizers in agriculture.

Clinical and electroencephalogram characteristics and treatment outcomes in children with benign epilepsy and centrotemporal spikes.

BACKGROUND: Epilepsy is a syndrome characterized by transient, rigid, paroxysmal, and repetitive central nervous system dysfunction. Prevention, control, and improvement of cognitive and behavioral dysfunction are of great significance for improving the patients' intellectual development and quality of life. Electroencephalograms (EEG) can predict an accelerated decline in cognitive function. AIM: To determine the clinical and EEG characteristics and treatment results of benign epilepsy in spiking children. METHODS: A total of 106 cases of benign epilepsy in children with myocardial spines treated at our hospital from January 2017 to January 2020 were selected. Differences in clinical data and EGG characteristics between treatment-effective/-ineffective patients were analyzed, and children's intellectual development before and after treatment evaluated using the Gesell Development Diagnostic Scale. RESULTS: EEG showed that the discharge proportion in the awake and sleep periods was 66.04%, and the peak/peak discharge was mainly single-sided, accounting for 81.13%, while the discharge generalization accounted for 31.13%. There was no significant difference in any of these variables between sexes and ages (P > 0.05). The proportion of patients with early onset (< 5 years old) and seizure frequency > 3 times/half a year was 40.00% and 60.00%, respectively; the incidence rate and seizure frequency in the younger age group (< 5 years old) were significantly higher than those in the treatment-effective group (P < 0.05), while the discharge index was significantly lower than that in the treatment-effective group (P < 0.05). The discharge index was negatively correlated with fine motor skill and language development (r = -0.274 and -0.247, respectively; P < 0.05), but not with the rest (P > 0.05). Logistic regression analysis showed that low age onset (< 5 years old) and seizure frequency were the factors affecting ineffective-treatment of benign epilepsy in children (odds ratio = 11.304 and 5.784, respectively; P < 0.05). The discharge index of the responsive group after treatment was significantly lower than that of the unresponsive group (P < 0.05). However, there was no significant difference between groups after treatment in gross and fine motor skills, adaptability, language, and personal social development (P > 0.05). CONCLUSION: The EEG of children with benign epilepsy due to spinal wave in central time zone has characteristic changes, and the therapeutic effect is influenced by age of onset and attack frequency.

[Effect of shock lymph on the expression of inflammatory mediators in pulmonary micro-vascular endothelial cells of rats].

OBJECTIVE: To observe the effect of lymph collected during shock on free radical and expressions of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) mRNA of pulmonary micro-vascular endothelial cells (PMVECs) of rats in order to explore the mechanisms of damaging effect of lymph collected during shock to the PMVECs. METHODS: PMVECs were isolated and cultured, and used at passage 3. The model of serious hemorrhagic shock was reproduced by maintaining the arterial blood pressure of rats at 40 mm Hg (1 mm Hg=0.133 kPa) for 90 minutes by exsanguination under aseptic condition. Mesentery lymph and portal vein blood were obtained from both shock rats and normal rats. PMVECs were respectively incubated in them for 6 hours, and at the same time, fetal bovine serum (FBS) and serum-free DMEM were used as culture media for comparison. The expressions of inducible nitric oxide synthase (iNOS), TNF-alpha and IL-6 mRNA were detected by the method of reverse transcription-polymerase chain reaction (RT-PCR), and the content of malondialdehyde (MDA), NO, TNF-alpha and IL-6 in culture supernatants were determined. RESULTS: After the PMVECs was treated by shock lymph at a final concentration of 4% for 6 hours, the expressions of iNOS, TNF-alpha and IL-6 mRNA in PMVECs and the contents of MDA, NO, TNF-alpha and IL-6 in culture supernatant fluids in shock lymph group were significantly increased compared with those of FBS group, normal lymph group, shock plasma group, normal plasma group and DMEM group. At the same time, the expressions of iNOS, TNF-alpha and IL-6 mRNA in PMVECs and the contents of NO in culture supernatant fluid of shock plasma group were significantly increased compared with those of FBS group, normal lymph group, normal plasma group and DMEM group (P<0.05 or P<0.01). CONCLUSION: The results demonstrate that the shock lymph in final concentration of 4% could enhance the expressions of iNOS, TNF-alpha and IL-6 of PMVECs, reduce the free radical, and as a result, induce damage to PMVECs.

[Effect of ligation of mesenteric lymph duct on inflammation response of liver in hemorrhagic shock in rats].

OBJECTIVE: To observe the effect of ligation of mesenteric lymph duct on changes in free radicals and pro-inflammatory mediators in the liver of rats with serious hemorrhagic shock at different periods, and explore the effect of blockage of intestinal lymphatic pathway on inflammation response of liver. METHODS: Seventy-eight male Wistar rats were randomly divided into the sham group (n=6), shock group (n=42), and ligation group (n=30). The model of serious hemorrhagic shock was reproduced in shock group and ligation group. Mesenteric lymph was blocked by ligating mesenteric lymph duct in ligation group after resuscitation. Six rats were sacrificed at 0, 1, 3, 6, 12, and 24 hours, and the livers were harvested and homogenized for the determination of malondialdehyde (MDA), nitric oxide (NO), nitric oxide synthase (NOS), superoxide dismutase (SOD), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and myeloperoxidase (MPO) activity. The expression of inducible nitric oxide synthase (iNOS) mRNA in liver was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The contents of TNF-alpha, IL-6, NO, NOS, MDA, MPO and iNOS mRNA in liver homogenate of shock group were increased after transfusion and resuscitation, and their levels were higher at 6 and 12 hours. The values were significantly higher than those of the sham group, while the activity of SOD was significantly lower than that of sham group (P<0.05 or P<0.01). The contents of TNF-alpha, IL-6, NO, NOS, MDA, MPO and iNOS mRNA in liver homogenate were lower significantly after transfusion and 3, 6, 12 and 24 hours after resuscitation than those of shock group at each time points, and the SOD activity was higher (P<0.05 or P<0.01). CONCLUSION: The results demonstrate that the ligation of mesenteric lymph duct could reduce the polymorphonuclear leucocyte (PMN) detain, and its mechanism might relate to reduction of neutrophil aggregation, thus decreases the release of TNF-alpha and IL-6, reduces the NO and expression of iNOS mRNA, reduces the release of free radicals and consumption of SOD, as a result, it reduces the inflammation response of liver in serious hemorrhagic shock rats.

Serum is an indispensable factor in the maintenance of the biological characteristics of sweat gland cells.

The tolerance of sweat gland cells for in vitro amplification and subcultivation is low as they are somatic cells. The present study aimed to formulate an optimal medium for the culture of human eccrine sweat gland cells (HESGCs) and to establish a method for induction of HESGCs proliferation, whilst maintaining the characteristics of sweat gland cells. HESGCs cultured in sweat gland (SG):keratinocyte growth medium­2 (KGM­2) (1:1) medium had a higher proliferation rate and a stable morphology compared with cells cultured in SG and KGM­2 medium only. Reverse transcription­quantitative polymerase chain reaction indicated that cells cultured in the SG:KGM­2 (1:1) medium exhibited higher expression levels of α­smooth muscle actin, keratin (K)77, carcinoembryonic antigen, K8, K18, ectodysplasin A receptor, c­Myc, Kruppel­like factor 4 and octamer­binding transcription factor 4 compared with cells cultured in SG only or KGM­2 only medium. Three­dimensional culture analysis revealed that HESGCs cultured in SG:KGM­2 1:1 medium differentiated into sweat gland­like structures, whereas cells cultured in KGM­2 only medium underwent cornification. The present study also determined that the maintenance of the biological characteristics of HESGCs occurred due to the presence of fetal bovine serum (FBS). Cells cultured in medium without FBS differentiated into keratinocytes. Therefore, the SG:KGM­2 (1:1) medium may be a suitable culture medium for HESGCs. In conclusion, this mixed medium is a valuable compound and should be considered to be a potential supplemental medium for HESGCs.

[Effect of mesenteric lymph duct ligation on nitric oxide content and its expression in lung in rats with hemorrhagic shock].

OBJECTIVE: To observe the effect of mesenteric lymph duct ligation on nitric oxide (NO) and its expression in lung in rats with serious hemorrhagic shock at different periods, and explore the role of intestinal lymphatic pathway in acute lung injury (ALI). METHODS: Seventy-eight male Wistar rats were randomly divided into the sham operation group (n=6), shock group (n=42), and ligation group (n=30). The model of serious hemorrhagic shock was reproduced in shock group and ligation group. Mesenteric lymph was diverted by ligating mesenteric lymph duct in ligation group after resuscitation. All rats were executed and their lungs were harvested. NO and nitric oxide synthase (NOS) were determined in lung homogenate 90 minutes after shock, and 0, 1, 3, 6, 12 and 24 hours after resuscitation. The expression of inducible nitric oxide synthase (iNOS) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: The content of NO, activity of NOS and expression of iNOS mRNA in lung tissue in shock group were increased 3 hours after transfusion and resuscitation, and they became higher 6-12 hours after resuscitation. The values were significantly higher than those in sham operation group, 90 minutes after shock and 0 hour after transfusion and resuscitation (P<0.05 or P<0.01). The values were only increased 3 hours and 6 hours after transfusion and resuscitation, and all the values on 6, 12 and 24 hours after resuscitation were significantly lower in the ligation group compared with those in shock group at the same time points (P<0.01 or P<0.05). CONCLUSION: The results demonstrate that the ligation of mesenteric lymph duct could reduce the NO and expression of iNOS mRNA in the lung tissue, thus ameliorate ALI in rats with serious hemorrhagic shock. Mesenteric lymph might play an important role in the pathogenesis of ALI.

Amniotic fluid stem cells provide considerable advantages in epidermal regeneration: B7H4 creates a moderate inflammation microenvironment to promote wound repair.

The current treatments for severe skin injury all involve skin grafting. However, there is a worldwide shortage of donor skin tissue. In this study, we examined the advantages of using human amniotic fluid stem (hAFS) cells in skin wound healing. In vitro, hAFS cells differentiate into keratinocytes (termed hAFS-K). Like keratinocytes, hAFS-K cells express the markers K5, K14, K10 and involucrin; display typical cellular structure, including a tonofibril-rich cytoplasm; and construct a completely pluristratified epithelium in 3D culture. In vivo, in a mouse excisional wound model, GFP-positive hAFS cells participate in wound repair. Co-localization of GFP/K14 and GFP/K10 in the repaired epidermis demonstrated that hAFS cells can differentiate into keratinocytes. Real-time PCR results confirmed that hAFS cells can initiate and promote early-stage repair of skin damage. During wound repair, hAFS cells did not directly secrete repair-related factors, such as bFGF, VEGF, CXCL12, TGF-ß1 and KGF, and provided a moderate inflammation reaction with lower expression of IL-1ß, IL-6, TNF-α, Cox2 and Mac3. In hAFS cells, the negative co-stimulatory molecule B7H4 regulates low immunogenicity, which can provide a modest inflammatory reaction microenvironment for wound repair. Furthermore, with their uniquely high proliferation rate, hAFS cells offer a promising alternative for epidermal regeneration.

[Effect of tetromethylpyrazine and aminoguanidine on expression of vascular endothelial growth factor in kidney of diabetic rats].

OBJECTIVE: To investigate the therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy. METHODS: Diabetic rats were induced by streptozotocin. They were divided into 5 groups: normal control group (group C), untreated diabetic group (group DM), tetromethylpyrazine treated group (group TMP), aminoguanidine treated group (group AG) and tetromethylpyrazine and aminoguanidine treated group (group TMP+AG). The expression of vascular endothelial growth factor (VEGF) in renal cortex of the rats in each group was observed by immunohistochemical staining after 12 weeks. RESULTS: The expression of VEGF in renal cortex of the rats in group TMP+AG and group C was alike. The expression of VEGF in renal cortex of group TMP and group AG decreased significantly as compared with that of group C, but was still above normal level. CONCLUSION: The therapeutic mechanism of tetromethylpyrazine and aminoguanidine on diabetic nephropathy may be inhibiting the over-expression of VEGF in kidney of diabetic rats.

Serum levels of 25-hydroxyvitamin D are associated with cognitive impairment in type 2 diabetic adults.

Hypovitaminosis D is highly prevalent in type 2 diabetes. The aim of this study is to determine the serum levels of 25-hydroxyvitamin D [25(OH)D] in type 2 diabetic patients with and without mild cognitive impairment (MCI), and examine the relationship of 25(OH)D and MCI with other clinical factors. One hundred and sixty-five diabetic patients were enrolled in this study. Among whom, 95 patients were considered as MCI [Montreal Cognitive Assessment score (MoCA) < 26] and the other 70 as no MCI (MoCA ≥ 26). Subjects were assessed clinically. Diabetic patients with MCI had a longer duration of DM, fewer years of education, elevated fasting blood glucose (FBG), resistant index (RI) of carotid, and lower levels of 25(OH)D {[17.35 (13.02-25.92) vs 28.00 (19.67-34.30)] ng/ml, P < 0.001}. The MoCA score was positively correlated with log10[25(OH)D], education year, and inversely correlated with duration of DM, history of hypertension, intima-media thickness (IMT), FBG, max-RI, and min-RI. Log10[25(OH)D] was positively correlated with MoCA score, and inversely correlated with IMT, in multivariate regression analysis adjusted for age, sex, and education year, 25(OH)D (ß = 0.210, P = 0.003), history of hypertension (ß = -0.191, P = 0.007), IMT (ß = -0.194, P = 0.007), and FBG (ß = -0.157, P = 0.026) independently predicted MoCA score. In conclusion, our results suggest that levels of serum 25(OH)D are inversely associated with the cognitive impairment in diabetic patients. Vitamin D may be a potential protective factor for cognitive impairment in patients with type 2 diabetes.

Correlations between serum levels of 25-hydroxyvitamin D and carotid atherosclerosis in patients with type 2 diabetes in Shanghai.

OBJECTIVE: To explore the potential association between the serum levels of 25-hydroxyvitamin D [25(OH)D] and carotid atherosclerosis in patients with type 2 diabetes. MATERIAL AND METHODS: Three hundred and fifty patients with type 2 diabetes were enrolled in this study in Shanghai, China. B-mode ultrasound was used to detect carotid plaques as indicators of atherosclerosis and measure carotid artery intima-media wall thickness (C-IMT) at two sites of carotid artery. Subjects were divided into group A (patients with carotid plaques) and group B (patients without carotid plaques) and be assessed clinically. Serum levels of 25(OH)D and other clinical parameters were measured. Multivariate logistic regression was performed to find predictors of carotid atherosclerosis in the entire group. RESULTS: The levels of serum 25(OH)D were lower in group A than in group B[19.60 (13.30-25.73) vs 23.19 (18.10-30.06)ng/ml, P<0.001]. The C-IMT levels [(1.00±0.17 vs 0.88±0.20)mm, Ptrend<0.001] and proportion of people with carotid plaques(44/88 vs 20/87, Ptrend<0.001) in the lowest quartile of 25(OH)D were higher than in the highest quartile. Vitamin D concentrations were inversely associated with HbA1c in women(r=-0.194, P=0.006), and C-IMT in men(r=-0.409, P<0.001). Logistic regression analysis showed age, male sex, current smoke, history of hypertension, SBP, LDL-C and lg[25(OH)D] (OR: 0.924, 95%CI: 0.893-0.955, P<0.001) were independently associated with the presence of carotid plaques in T2DM. CONCLUSIONS: Serum vitamin D level is significantly and independently associated with carotid atherosclerosis in patients with T2DM in Shanghai, China.

Risk factors of mild cognitive impairment in middle aged patients with type 2 diabetes: a cross-section study.

UNLABELLED: The aim of this study was to evaluate the risk factors of mild cognitive impairment (MCI) in middle-aged patients with type 2 diabetes (T2DM). METHODS: Montreal Cognitive Assessment (MoCA) was applied as cognition assessment implement. One hundred and fifty-seven middle-aged type 2 diabetic patients were enrolled in this cross-section study (age 40~69, mean age 55 ± 7). There were 93 patients with MCI (MoCA score<26) in MCI group and 64 with normal cognitive function (MoCA score ≥ 26) in control group. Information of history of disease, family history, data of BMI, WHR, HbA1c, FINS, C-Peptide (C-P), SBP, DBP, blood lipid (TG, TC, LDL-C, HDL-C and carotid ultrasound (carotid IMT, carotid resistance index [RI]) was collected. RESULTS: There were significant differences in the rate of patients with hypertension ([40.63 vs. 58.06%], P=0.026), duration of diabetes mellitus ([3.09 ± 4.04 y vs. 4.80 ± 4.94 y], P=0.024), C-P ([2.79 ± 1.09 ng/ml vs. 2.26 ± 1.00 ng/ml], P=0.008), Max C-IMT ([0.81 ± 0.15 mm vs. 0.91 ± 0.15 mm], P<0.001), Min C-RI (0.71 ± 0.06 vs. 0.68 ± 0.06, P<0.05), and no significant differences in the duration of hypertension and hyperlipidemia, BMI, WHR, HbA1c, SBP, DBP and blood lipid between control group and MCI group. MoCA scores were positively correlated with C-P (r=0.252, P=0.005), and negatively correlated with the history of hypertension (r=-0.244, P=0.002), duration of DM (r=-0.161, P=0.044), Max C-IMT (r=-0.253, P=0.005) and Min C-RI (r=-0.183, P=0.023). Multiple regression analysis showed that history of hypertension (Beta=-0.267, P=0.002), C-P (Beta=0.281, P=0.001) and Min C-RI (Beta=-0.221, P=0.011) were significantly independent determinants for the MoCA scores. CONCLUSIONS: The longer duration of diabetes, history of hypertension, lower serum C-P levels, thickened C-IMT and higher C-RI could be risk factors of MCI in type 2 diabetic patients. This finding could have an important impact on the management of cognitive decline in diabetic patients.

Clinical study on the treatment of knee osteoarthritis of Shen-Sui insufficiency syndrome type by electroacupuncture.

OBJECTIVE: To study the clinical effificacy of electroacupuncture (EA) on treating knee osteoarthritis (KOA) of Shen ()-Sui () insuffificiency (SSI) syndrome type. METHODS: A total of 245 patients (279 knees) of KOA-SSI were randomly assigned to two groups by lottery: 141 knees in the treatment group and 138 knees in the control group. The treatment group was managed with EA at the dominant points of Neixiyan (Ex-LE4) and Waixiyan (Ex-LE5) as well as the conjugate points of Xuanzhong (GB39) and Taixi (KI3) for 30 min, once a day, with 15 days as one course; 2 courses were applied with a 5-day interval in between. The control group was treated with intra-articular injection of 2 mL hyaluronic acid into the affected joint every 7 days for 5 times in total. The clinical effects on the patients in different stages were observed, and their symptom scores of knee and contents of cytokines, including interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), prostaglandin E(2alpha) (PGE(2alpha)) and matrix metalloproteinases-3 (MMP-3), in the knee joint fluid were measured before and after treatment. RESULTS: The study was completed in 235 patients (263 knees); four patients (7 knees) in the treatment group and six patients (9 knees) in the control group dropped out. Comparison of therapeutic effects (excellent and effective rates) between the two groups showed insignificant differences (P>0.05). Symptom scores of knee and contents of cytokines in the knee flfluid after treatment were lowered signifificantly in the patients of stage I-III in both groups (P<0.05 or P<0.01). However, the lowering of the total symptom score of knee in the patients of stage III in the treatment group was more signifificant (P<0.05). CONCLUSIONS: EA could effectively alleviate the clinical symptoms in KOA patients of stage III, showing an effect superior to that of hyaluronic acid. EA also shows action in suppressing the secretion of IL-1, IL-6, TNF-alpha, PGE(2alpha) and MMP-3 in the knee flfluid.

[Effects of shock lymph inducing the mesentery micro-lymphatic endothelial cells apoptosis of rats].

The model of serious hemorrhagic shock was established in the condition of asepsis and mesentery lymph was taken out. As control, normal mesentery lymph fluid, normal portal vein blood and shock portal vein blood of rats were taken out. The primary mesentery micro-lymphatic endothelial cells (MMLECs) of passages 3 were treated by different treatment factors, respectively. The cells proliferation by shock lymph and normal lymph of different final concentrations was measured using MTT method. The cell cycle arrest was analyzed by flow cytometry, and the electrophoresis analyze on DNA of cell nucleus was observed. The expressions of relative genes of apoptosis such as fas, fasL, bcl-2 and bax of MMLECs were detected by RT-PCR. The results showed that the MMLECs proliferation was decreased when the concentration of shock lymph increased to some extent and showed statisticly significant difference compared with normal lymph group. The cells cocultured with shocking lymph fluid at 4% final concentration showed that apoptosis rate of MMLECs was (35.4 +/- 1.6)%, and G0-G1 cell population was higher and the proportion of S+G2-M cell population was lower than that of other groups, and the DNA ladder was observed in electrophoresis of cell nucleus DNA at same time. And the expressions level of fas, fasL and bax mRNA were higher and bcl-2 mRNA was lower in shock lymph group than that of control group. The results demonstrated that shock lymph could reduce the cells proliferation, interfere with cell cycle, and accelerate high expression of apoptosis accelerative genes. As a result, shock lymph could induce the MMLECs apoptosis.