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Ann Hematol ; 100(11): 2677-2682, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34519886

RESUMO

Immune thrombocytopenia (ITP) is an acquired bleeding disorder, for which no specific diagnostic test exists. Inherited thrombocytopenia (IT) can mimic ITP and lead to unappropriated management with significant morbidity. Here, in small cohorts of these two disorders, we explored whether platelet sialylation and platelet activation could allow to discriminate the two conditions. We also aimed to confirm the value of immature platelet counts in this discrimination. Platelet sialylation and the expression level of P-selectin were assessed by multiparameter flow cytometry. Immature platelets were estimated on a Sysmex XN 9000 analyzer. No significant difference in platelet sialylation was observed between ITP and IT. Contrarily, platelet activation was significantly higher in ITP patients (p = 0.008). The immature platelet fraction, as previously demonstrated, was significantly lower in the ITP group compared to the IT group (p = 0.014). That statistical significance was achieved in this small pilot study suggests that the two easily available assays of immature platelet count and P-selectin expression could help physicians to reach the proper diagnosis in complex cases of thrombocytopenia.


Assuntos
Plaquetas/química , Ativação Plaquetária , Ácidos Siálicos/sangue , Trombocitopenia/sangue , Adulto , Idoso , Área Sob a Curva , Síndrome de Bernard-Soulier/sangue , Síndrome de Bernard-Soulier/diagnóstico , Síndrome de Bernard-Soulier/genética , Senescência Celular , Diagnóstico Diferencial , Feminino , Citometria de Fluxo/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Selectina-P/sangue , Projetos Piloto , Contagem de Plaquetas , Púrpura Trombocitopênica Idiopática/sangue , Púrpura Trombocitopênica Idiopática/diagnóstico , Curva ROC , Sensibilidade e Especificidade , Trombocitopenia/diagnóstico , Trombocitopenia/genética
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