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OBJECTIVES: Encephalitis is a severe neurological syndrome for which herpesvirus and enteroviruses are the most common etiological agents. Arboviruses, a wildly diverse group of pathogens, are also critical epidemiological agents associated with encephalitis. In Brazil, little is known about the causative agents of encephalitis. METHODS: We conducted a hospital surveillance for encephalitis between 2020 and 2022. Molecular (RT-PCR and qPCR) and serological (virus-specific IgM and viral antigens) techniques were performed in cerebrospinal fluid and serum samples obtained from study participants. RESULTS: In the 43 participants evaluated, the etiologic agent or the presence of IgM was detected in 16 (37.2%). Nine (20.9%) cases were positive for chikungunya virus (CHIKV), three (7.0%) for dengue virus, two (4.7%) for human adenovirus, one (2.3%) for varicella-zoster virus, and one (2.3%) for enterovirus. Whole-genome sequencing revealed that the CHIKV identified belongs to the East/Central/South African lineage. CONCLUSION: Herein, CHIKV is a common pathogen identified in encephalitis cases. Our results reinforce previous evidence that chikungunya represents a significant cause of encephalitis during CHIKV outbreaks and epidemics and add to existing information on the epidemiology of encephalitis in Brazil.
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Febre de Chikungunya , Vírus Chikungunya , Humanos , Brasil/epidemiologia , Vírus Chikungunya/genética , Vírus Chikungunya/isolamento & purificação , Masculino , Feminino , Febre de Chikungunya/epidemiologia , Febre de Chikungunya/virologia , Febre de Chikungunya/diagnóstico , Febre de Chikungunya/sangue , Adulto , Adolescente , Criança , Adulto Jovem , Pessoa de Meia-Idade , Pré-Escolar , Anticorpos Antivirais/sangue , Encefalite Viral/epidemiologia , Encefalite Viral/virologia , Encefalite Viral/diagnóstico , Imunoglobulina M/sangue , Idoso , Vírus da Dengue/genética , Vírus da Dengue/isolamento & purificação , Lactente , Filogenia , Herpesvirus Humano 3/genética , Herpesvirus Humano 3/isolamento & purificação , Enterovirus/isolamento & purificação , Enterovirus/genética , Sequenciamento Completo do GenomaRESUMO
Praziquantel (PZQ) is a medication used to treat several parasitic infections, including human schistosomiasis. Although this drug commonly causes transient adverse effects, severe hypersensitivity is rare, and only eight cases have been reported worldwide. Herein we report a case of a 13-year-old Brazilian female who developed anaphylaxis, a severe hypersensitive reaction, after taking praziquantel to treat Schistosoma mansoni infection. During a mass drug administration event in a socially vulnerable endemic area of Bahia (Brazil), after taking 60 mg/kg of praziquantel the patient developed rash and generalized edema an hour later, evolving to somnolence and hypotension. Following the anaphylactic episode, she received adequate treatment and recovered approximately 1 day later. Although praziquantel is considered safe, health professionals should be aware of potential life-threatening adverse events.
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Background: Despite growing scientific knowledge of Zika virus (ZIKV) infection, questions remain regarding ZIKV infection in pregnancy and congenital ZIKV syndrome (CZS). Methods: The ZIKAction Paediatric Registry is an international registry of children with documented ZIKV exposure in utero and/or with confirmed or suspected CZS. Its aim is to characterize these children (i.e., clinical, radiological, neurodevelopmental features) and describe outcomes, longer-term sequelae and management through retrospective case note review. This analysis described the maternal and perinatal characteristics of children in the Registry's Bahia arm, assessed their neuroimaging, ophthalmic, hearing and electroencephalography abnormalities by microcephaly classification and reported on hospitalisations. Children born in 2015-2018 and enrolled 2020-2021 in three public health facilities in Salvador were included. Results: Of 129 (57% female) children, 15 (11·6%) had laboratory-confirmed congenital ZIKV infection and 114 (88·4%) suspected CZS. At delivery, 15 (11·6%) were normocephalic, 30 (23·3%) moderately microcephalic, and 84 (65·1%) severely microcephalic. Median birth head circumference z-score was -3·51 [IQR, -4·69,-2·73]. During follow-up, all children had abnormal neuroimaging, 80·3% (94/117) abnormal electroencephalogram, 62·2% (77/120) ophthalmic abnormalities, and 27·4% (34/124) hearing impairment. Microcephaly classification was significantly associated with gestational age, and ophthalmological and electroencephalography abnormalities. Of 125 children with hospitalisation data, 52 (41·6%) had been hospitalised by most recent follow-up, at median age of 15·8 [4·0, 34·4] months; infections were the leading cause. Conclusion: Congenital ZIKV infection is an emerging disease with a varied and incompletely understood spectrum. Continued long-term follow-up is essential to understand longer-term prognosis and to inform future health and educational needs.
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Dengue fever is among the most significant public health concerns in Brazil. To date, the highest number of Dengue notifications in the Americas has been reported in Brazil, with cases accounting for a total number of 3,418,796 reported cases as of mid-December 2022. Furthermore, the northeastern region of Brazil registered the second-highest incidence of Dengue fever in 2022. Due to the alarming epidemiological scenario, in this study, we used a combination of portable whole-genome sequencing, phylodynamic, and epidemiological analyses to reveal a novel DENV-1 genotype V clade and the persistence of DENV-2 genotype III in the region. We further report the presence of non-synonymous mutations associated with non-structural domains, especially the NS2A (non-structural protein 2A), as well as describe synonymous mutations in envelope and membrane proteins, distributed differently between clades. However, the absence of clinical data at the time of collection and notification, as well as the impossibility of monitoring patients in order to observe worsening or death, restricts our possibility of correlating mutational findings with possible clinical prognoses. Together, these results reinforce the crucial role of genomic surveillance to follow the evolution of circulating DENV strains and understand their spread across the region through inter-regional importation events, likely mediated by human mobility, and also the possible impacts on public health and outbreak management.
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Vírus da Dengue , Dengue , Humanos , Vírus da Dengue/genética , Filogenia , Dengue/epidemiologia , Brasil/epidemiologia , Variação Genética , RNA Viral/genética , GenótipoRESUMO
BACKGROUND: Salvador was one of the Brazilian cities most affected during the 2015 Zika virus (ZIKV) outbreak. METHODS: A cross-sectional study was performed with enrolment of parturients and their newborns. RESULTS: Positive IgM antibodies for ZIKV, dengue (DENV) and Chikungunya (CHIKV) were present in 6.9, 11.9 and 22.8% of the parturients, and IgG antibodies were detected in 72.3, 92.3 and 38.6%, respectively. No cases of DENV congenital infection were identified. ZIKV and CHIKV congenital infections were observed in 16.5 and 13% of newborns, respectively. CONCLUSIONS: High exposure rates to the three arboviruses and the identification of newborns with ZIKV and CHIKV congenital infections reinforces the necessity of ZIKV and CHIKV prenatal and neonatal screening in endemic regions.
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Febre de Chikungunya , Dengue , Infecção por Zika virus , Anticorpos Antivirais/sangue , Brasil/epidemiologia , Febre de Chikungunya/congênito , Febre de Chikungunya/epidemiologia , Estudos Transversais , Dengue/congênito , Dengue/epidemiologia , Feminino , Humanos , Imunoglobulina G/sangue , Recém-Nascido , Gravidez , Infecção por Zika virus/congênito , Infecção por Zika virus/epidemiologiaRESUMO
Dengue é uma doença endêmica no Brasil há décadas. Entre os anos de 2015 e 2016 o Brasil foi afetado por um surto de infecção pelo vírus Zika (ZIKV) sem precedentes, que atingiu diversas regiões do país, incluindo o estado da Bahia e sua capital Salvador. Um dos grupos populacionais mais susceptíveis é o de gestantes, devido ao risco de infecção congênita por Zika.
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Doenças transmitidas por vetores levam a óbito cerca de 1 milhão de indivíduos por ano. Há um grande risco de infecção por vírus transmitidos por mosquitos Aedes em cidades nas quais esses encontram condições favoráveis para proliferação . Dessas arboviroses , a doença aguda pelo vírus Zika (ZIKV) , dengue (DENV) e febre de chikungunya (CHIKV) vêm tornando - se preocupação no cenário mundial , devido a fatores , como sua rápida propagação geográfica e os altos custos gerados aos sistemas de saúde.