RESUMO
Karyotyping is an examination that helps in detecting chromosomal abnormalities. Chromosome analysis is a very challenging task which requires various steps to obtain a karyotype. The challenges associated with chromosome analysis are overlapping and touching of chromosomes. The input considered for chromosome analysis is the metaspread G band chromosomes. The proposed work mainly focus on separation the overlapped and touching chromosomes which is considered to be the major challenge in karyotype. There are various research contribution in chromosome analysis in progress which includes both low (Machine Learning) and high level (Deep Learning) methods. This paper proposes a mathematical based approaches which is very effective in segmentation of clustered chromosomes. The accuracy of segmentation is robust compared to high level approaches.