Clinical and Ultrasound Efficacy of Topical Hypertonic Cream (Jovita Osmocell®) in the Treatment of Cellulite: A Prospective, Monocentric, Double-Blind, Placebo-Controlled Study.

Background and Objectives: Cellulite, or edemato-fibro-sclerotic panniculopathy (EFP), is characterized by dermal and hypodermal changes leading to adipose tissue accumulation and compromised venous circulation. This study investigates the efficacy of a hypertonic cream containing concentrated sodium chloride (Jovita Osmocell®) in addressing water retention and structural alterations in adipose tissue, aiming to interrupt the cellulite formation process. Materials and Methods: A 12-week, prospective, monocentric, double-blind, placebo-controlled study enrolled 30 female subjects with grade II or III cellulite. Patients were randomized to receive hypertonic cream or a placebo. Thigh circumference, ultrasound evaluations, and standardized photographs were collected at baseline, intermediate, and endpoint visits. Adverse events were monitored. Results: After 84 days, the hypertonic cream group exhibited a significant reduction in thigh circumference compared to the placebo group (p = 0.0037). B-mode ultrasound examinations revealed significant changes in the parameters studied, such as the thickness of the subcutaneous tissue. No statistically significant changes were noticed in the placebo group. Volunteers reported the investigational product's pleasantness and good anti-cellulite activity, with no reported adverse events. Conclusions: The hypertonic cream demonstrated efficacy in reducing thigh circumference, addressing water retention and structural alterations in adipose tissue. The proposed mechanism involves osmosis, releasing accumulated fluids between fat cells, supporting drainage, and reducing inflammation. This study supports the efficacy and safety of hypertonic sodium chloride emulsions in cellulite treatment and confirms safety and user satisfaction.

New Data Set of Polychlorinated Dibenzo-p-dioxin and Dibenzofuran Half-Lives: Natural Attenuation and Rhizoremediation Using Several Common Plant Species in a Weathered Contaminated Soil.

In this paper, a new data set of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/Fs) half-lives (HLs) in soil is presented. Data are derived from a greenhouse experiment performed with an aged contaminated soil under semi-field conditions, obtained from a National Relevance Site (SIN) located in Northern Italy (SIN Brescia-Caffaro). Ten different treatments (combination of seven plant species with different soil conditions) were considered together with the respective controls (soil without plants). The ability of the plants to stimulate the biodegradation of these compounds was evaluated by measuring the PCDD/F concentration reduction in soil over a period of 18 months. The formation of new bound residues was excluded by using roots as a passive sampler of bioaccessible concentrations. The best treatment which significantly reduced PCDD/F concentrations in soil was the one with Festuca arundinacea (about 11-24% reduction, depending on the congener). These decreases reflected in HLs ranging from 2.5 to 5.8 years. Simulations performed with a dynamic air-vegetation-soil model (SoilPlusVeg) confirmed that these HLs were substantially due to biodegradation rather than other loss processes. Because no coherent PCDD/F degradation HL data sets are currently available for soil, they could substantially improve the predictions of soil remediation time, long-range transport, and food chain transfer of these chemicals using multimedia fate models.

Identification of Sulfonated and Hydroxy-Sulfonated Polychlorinated Biphenyl (PCB) Metabolites in Soil: New Classes of Intermediate Products of PCB Degradation?

In this paper we describe the identification of two classes of contaminants: sulfonated-PCBs and hydroxy-sulfonated-PCBs. This is the first published report of the detection of these chemicals in soil. They were found, along with hydroxy-PCBs, in soil samples coming from a site historically contaminated by the industrial production of PCBs and in background soils. Sulfonated-PCB levels were approximately 0.4-0.8% of the native PCB levels in soils and about twice the levels of hydroxy-sulfonated-PCBs and hydroxy-PCBs. The identification of sulfonated-PCBs was confirmed by the chemical synthesis of reference standards, obtained through the sulfonation of an industrial mixture of PCBs. We then reviewed the literature to investigate for the potential agents responsible for the sulfonation. Furthermore, we predicted their physicochemical properties and indicate that, given the low pKa of sulfonated- and hydroxy-sulfonated-PCBs, they possess negligible volatility, supporting the case for in situ formation from PCBs. This study shows the need of understanding their origin, their role in the degradation path of PCBs, and their fate, as well as their (still unknown) toxicological and ecotoxicological properties.

Estimation of polycyclic aromatic hydrocarbon variability in air using high volume, film, and vegetation as samplers.

Organic films and leaves provide a medium into which organic contaminants, such as polycyclic aromatic hydrocarbons (PAHs), can accumulate, resulting in a useful passive air sampler. In the present work, the temporal variability (weekly) in PAH concentrations and the fingerprint of films developed on window surfaces were investigated. Moreover, films and leaves of two tree species (Acer pseudoplatanus and Cornus mas) collected at the same time were used to derive PAH air concentrations and investigate their short-term variability. In general, the most abundant chemicals found in films were phenanthrene and pyrene (22%), followed by perylene (21%) and fluoranthene (16%), but the fingerprint (in contrast to leaves and air) changed over time. Leaf derived air concentrations were within a factor of 2 to 9 from measured values, while air concentrations back-calculated from films were within a factor of 2 to 53. This happened because predicted air concentrations using films and vegetation samplers (especially for low KOA chemicals) generally reflect only the last few hours (due to the fast equilibrium) of the weekly integrated samples obtained employing the high-volume sampler. This means that films and leaves can be usefully employed for predicting the short-term variability of low KOA organic contaminant air concentrations.

Comparison of microplastic type, size, and composition in atmospheric and foliage samples in an urban scenario.

The rising trend of plastic production in last years and the inadequate disposal of related waste has raised concerns regarding microplastic-related environmental issues. Microplastic particles disperse by means of transport and deposition processes to different ecosystems and enter food chains. In this paper, atmospheric deposition and foliage samples of two species (i.e., Hedera helix and Photinia glabra) were collected and analysed for the quantity and identity of microplastics (MPs). A preliminary methodology to treat foliage samples and subsequently identify MPs using a quantum cascade laser IR spectrophotometer is presented. The treatment of airborne samples involved filtration, mild digestion, concentration, and transfer onto reflective slides whereas that for foliage involved washing, concentration, and transference of putative MPs onto reflective slides. Fibers and fragments were differentiated according to their physical features (size, width, height, etc.) and calculating derived characteristics (namely, circularity and solidity). The preliminary results obtained suggest a good agreement between atmospheric-deposited and foliage-retained MPs, showing the capability of leaves to act as passive samplers for environmental monitoring.

Relationship between foliar polycyclic aromatic hydrocarbons (PAHs) concentrations and plant traits: Intracanopy variability for a broadleaf species in an urban environment.

The emission of potentially harmful compounds, including polycyclic aromatic hydrocarbons (PAHs), and the resulting air pollution is a serious problem in modern cities. It is therefore important to develop mitigation strategies, such as "smart" planting of trees that act as sinks for PAHs. However, the intra-individual (within-tree) variability in leaf PAH concentrations remains unknown. In this paper, we studied 15 ornamental apple trees (Malus × moerlandsii 'Profusion') growing on a main street in a medium-sized city in Galicia (NW Spain). We determined the PAH concentrations at 12 canopy positions in each tree (2 orientations and 2 distances from the trunk at 3 heights), measured various ecological traits (specific leaf area [SLA], δ13C, stomatal density, fatty acid contents and leaf hairiness) and analyzed the variability in traits within the canopy in relation to PAH concentrations. We observed high intra-individual variability in the PAH concentrations and the leaf traits. Statistical analyses revealed that leaf height was the main source of variability both in the PAH concentrations and in the traits, mainly due to the leaf morphology, particularly to the SLA. Therefore, the ideal vegetation to remove PAHs would be high leaf biomass trees, not too tall and with a high proportion of shade leaves.

Diagnostic Imaging of Agminated Blue Lesions and Blue Lesions with Satellitosis: Case Series with a Concise Review of the Current Literature.

Background: Agmination and/or satellitosis in pigmented blue lesions is a phenomenon rarely mentioned in the literature and not well known. This phenomenon can be expressed by several benign and malignant pigmented blue lesions, such as blue nevi, Spitz nevi, melanocytoma and melanoma. On this spectrum, dermoscopy, reflectance confocal microscopy (RCM) and dynamic Optical coherence tomography (D-OCT) represent non-invasive imaging technologies, which may help clinicians in the diagnosis of melanoma and non-melanoma skin cancers in daily clinical practice. Methods: Currently, in the literature there is a lack of new data about agminated blue lesions and blues lesions with satellitosis, as well as the lack of a recent and updated review of the literature about this topic. Therefore, considering that clinicians must be confident with the diagnosis of these rare skin lesions, we decided to carry out this work. Results: In this paper, four new cases of agminated pigmented cutaneous lesions were described. Moreover, a review of the current literature on this topic was performed. Conclusions: A clinical-pathological correlation is often needed to reach a correct diagnosis; currently, dermoscopy and non-invasive diagnostic techniques, such as reflectance confocal microscopy and optical coherence tomography, due to the depth of these skin lesions in the dermis, can only make a partial and limited contribution.

Predicting the contribution of a local emission source in mid-range transport of DDT and its deposition in terrestrial and aquatic ecosystems in Northern Italy.

A recently developed dynamic multiple box multimedia fate model (Gridded-SoilPlusVeg, or GSPV) was developed and implemented to account for the environmental variation and the effect of directional advective transport of chemicals towards different compartments and geographical locations. A chemical plant located in Pieve Vergonte in Ossola Valley produced and emitted DDTs for around 50 years. In the previous study the fate and transport of p,p'-DDT emitted from the chemical plant were evaluated in nearby areas (up to 12 km). In this paper, the GSPV model was run for p,p'-DDT from its production period and decades after the production stop in 1996 (a total of 100 years) for a much larger study area (40,000 km2) in order to evaluate the contribution of a local source on a larger scale. Additionally, the deposition fluxes into the lakes were calculated and were used as input into a dynamic fugacity-based aquatic model to calculate DDT concentration in water and sediments of three Prealpine lakes: Lake Maggiore, Lake Como and Lake Lugano. The results of the simulations were compared with the monitoring and literature data. The results obtained from GSPV allowed to estimate the atmospheric deposition fluxes and identify the role of this source for the regional scale contamination in terrestrial and aquatic ecosystems.

Arsenic movement and fractionation in agricultural soils which received wastewater from an adjacent industrial site for 50 years.

Arsenic (As) is an element with important environmental and human health implications due to its toxic properties. It is naturally occurring since it is contained in minerals, but it can also be enriched and distributed in the environment by anthropogenic activities. This paper reports on the historic As contamination of agricultural soils in one of the most important national relevance site for contamination in Italy, the so-called SIN Brescia-Caffaro, in the city of Brescia, northern Italy. These agricultural areas received As through the use of irrigation waters from wastewater coming from a factory of As-based pesticides (lead and calcium arsenates, sodium arsenite). Pesticide production started in 1920 and ended in the '70. Concentrations in the areas are generally beyond the legal threshold values for different soil uses and are up to >200 mg/kg. Arsenic contamination was studied to assess the long-time trend and the dynamics related to the vertical movement of As down to 1 m depth and its horizontal diffusion with surface irrigation in the entire field. Arsenic fractionation analysis (solid phase speciation by sequential extraction procedure) was also performed on samples collected from these areas and employed in greenhouse experiments with several plant species to evaluate the long-term contamination and the role of plant species in modifying As availability in soil. The results of this work can help in the evaluation of the conditions controlling the vertical transfer of As towards surface aquifers, the bioaccumulation likelihood in the agricultural food chain and the selection of sustainable remediation techniques such as phytoextraction.

Environmental fate of sulfonated-PCBs: Soil partitioning properties, bioaccumulation, persistence, and mobility.

Two new classes of PCB metabolites were recently discovered: sulfonated-polychlorinated biphenyls (sulfonated-PCBs) and hydroxy-sulfonated-polychlorinated biphenyls (OH-sulfonated-PCBs). These metabolites, originating from PCB degradation, seem to possess more polar characteristics than their parent compounds. However, no other information, such as their chemical identity (CAS number) or their ecotoxicity or toxicity, is available so far, although more than about one hundred different chemicals were observed in soil samples. In addition, their physico-chemical properties are still uncertain since only estimations are available. Here we show the first evidence on the fate of these new classes of contaminants in the environment, producing results from several experiments, to evaluate sulfonated-PCBs and OH-sulfonated-PCBs soil partition coefficients, degradation in soil after 18 months of rhizoremediation, uptake into plant roots and earthworms, as well as a preliminary analytical method to extract and concentrate these chemicals from water. The results give an overview of the expected environmental fate of these chemicals and open questions for further studies.

Use of Next Generation Sequencing to Define the Origin of Primary Myelofibrosis.

Primary myelofibrosis (PMF) is a chronic myeloproliferative neoplasm (MPN) characterized by progressive bone marrow sclerosis, extra-medullary hematopoiesis, and possible transformation to acute leukemia. In the last decade, the molecular pathogenesis of the disease has been largely uncovered. Particularly, genetic and genomic studies have provided evidence of deregulated oncogenes in PMF as well as in other MPNs. However, the mechanisms through which transformation to either the myeloid or lymphoid blastic phase remain obscure. Particularly, it is still debated whether the disease has origins in a multi-potent hematopoietic stem cells or instead in a commissioned myeloid progenitor. In this study, we aimed to shed light upon this issue by using next generation sequencing (NGS) to study both myeloid and lymphoid cells as well as matched non-neoplastic DNA of PMF patients. Whole exome sequencing revealed that most somatic mutations were the same between myeloid and lymphoid cells, such findings being confirmed by Sanger sequencing. Particularly, we found 126/146 SNVs to be the e same (including JAK2V617F), indicating that most genetic events likely to contribute to disease pathogenesis occurred in a non-commissioned precursor. In contrast, only 9/27 InDels were similar, suggesting that this type of lesion contributed instead to disease progression, occurring at more differentiated stages, or maybe just represented "passenger" lesions, not contributing at all to disease pathogenesis. In conclusion, we showed for the first time that genetic lesions characteristic of PMF occur at an early stage of hematopoietic stem cell differentiation, this being in line with the possible transformation of the disease in either myeloid or lymphoid acute leukemia.

Synthesis of a new sulfonated-hexachlorobiphenyl standard for environmental analysis, ecotoxicological, and toxicological studies.

Sulfonated-polychlorinated biphenyls (sulfonated-PCBs) are a newly discovered class of PCB metabolites. They were observed for the first time in polar bear serum and lately, in soil, together with hydroxy-sulfonated-PCBs. Their presence is ubiquitous in soils, and their estimated physical chemical properties show high mobility in water, compared to the parent compounds. However, no single pure standards exist so far and therefore their quantification in the environmental matrices is not accurate. Additionally, pure standards are needed to experimentally determine their physical chemical properties, as well as the ecotoxicological and toxicological characteristics. In the present work, the challenging goal of preparing a polychlorinated biphenyl monosulfonic acid was achieved exploring different synthetic approaches, along which the selection of the starting material resulted in a crucial point. Using PCB-153 (2,2'-4,4'-5,5'-hexachloro-1,1'-biphenyl) the synthesis afforded, as the major species, a side compound. On the contrary, the use of PCB-155 (2,2'-4,4'-6,6'-hexachloro-1,1'-biphenyl), a symmetric hexachlorobiphenyl derivative showing chlorine atoms at all the ortho positions, gave the target sulfonated-PCB compound. In this case, sulfonation was successfully carried out through a two-step procedure, involving chlorosulfonylation and the subsequent hydrolysis of the chlorosulfonyl intermediate.

Spatially resolved environmental fate models: A review.

Spatially resolved environmental models are important tools to introduce and highlight the spatial variability of the real world into modeling. Although various spatial models have been developed so far, yet the development and evaluation of these models remain a challenging task due to several difficulties related to model setup, computational cost, and obtaining high-resolution input data (e.g., monitoring and emission data). For example, atmospheric transport models can be used when high resolution predicted concentrations in atmospheric compartments are required, while spatial multimedia fate models may be preferred for regulatory risk assessment, life cycle impact assessment of chemicals, or when the partitioning of chemical substances in a multimedia environment is considered. The goal of this paper is to review and compare different spatially resolved environmental models, according to their spatial, temporal and chemical domains, with a closer insight into spatial multimedia fate models, to achieve a better understanding of their strengths and limitations. This review also points out several requirements for further improvement of existing models as well as for their integration.

Predicting the regional contamination evolution of DDT for 100-years with a new gridded spatial and dynamic multimedia fate model.

In 1996 high dichlorodiphenyltrichloroethane (DDT) concentrations were found in Lake Maggiore (Italy) fish and sediments. DDT was produced by a chemical company located in a subalpine valley (Ossola valley, Piedmont Region, Italy), and ended up in the Toce River, a tributary of Lake Maggiore. In the area surrounding the chemical plant, high DDT concentrations in soil and vegetation were found after subsequent investigations. The quantification of the release from contaminated soil and the following migration toward downwind areas, deposition to the soil, and further evaporation plays an important role in understanding future DDT trends in soil and the atmosphere. To study this phenomenon, soil, and vegetation from Ossola Valley were monitored in 2001 and 2011. The concentration values obtained (soils: 0.05 to 1 µg/g; vegetation 2-100 ng/g), allowed to reconstruct the contamination gradient in the valley and were used to develop and calibrate a spatially resolved multimedia fugacity model. The model accounts for spatial and temporal dynamicity of environmental characteristics such as wind speed and direction, variable air compartment height etc., and simulates the fate and transport of chemicals on a local scale. The dynamic emission of DDT (about 13,000 kg for the 50 y production time) to the air was estimated and utilized for a 100-year simulation (from 1948 to 2048). The results obtained allowed to understand the temporal and spatial pattern of DDT contamination for a long period at a local scale as well as the potential contribution as a source potentially affecting sites at larger distances.

Enabling forecasts of environmental exposure to chemicals in European agriculture under global change.

European agricultural development in the 21st century will be affected by a host of global changes, including climate change, changes in agricultural technologies and practices, and a shift towards a circular economy. The type and quantity of chemicals used, emitted, and cycled through agricultural systems in Europe will change, driven by shifts in the use patterns of pesticides, veterinary pharmaceuticals, reclaimed wastewater used for irrigation, and biosolids. Climate change will also impact the chemical persistence, fate, and transport processes that dictate environmental exposure. Here, we review the literature to identify research that will enable scenario-based forecasting of environmental exposures to organic chemicals in European agriculture under global change. Enabling exposure forecasts requires understanding current and possible future 1.) emissions, 2.) persistence and transformation, and 3.) fate and transport of agricultural chemicals. We discuss current knowledge in these three areas, the impact global change drivers may have on them, and we identify knowledge and data gaps that must be overcome to enable predictive scenario-based forecasts of environmental exposure under global change. Key research gaps identified are: improved understanding of relationships between global change and chemical emissions in agricultural settings; better understanding of environment-microbe interactions in the context of chemical degradation under future conditions; and better methods for downscaling climate change-driven intense precipitation events for chemical fate and transport modelling. We introduce a set of narrative Agricultural Chemical Exposure (ACE) scenarios - augmenting the IPCC's Shared Socio-economic Pathways (SSPs) - as a framework for forecasting chemical exposure in European agriculture. The proposed ACE scenarios cover a plausible range of optimistic to pessimistic 21st century development pathways. Filling the knowledge and data gaps identified within this study and using the ACE scenario approach for chemical exposure forecasting will support stakeholder planning and regulatory intervention strategies to ensure European agricultural practices develop in a sustainable manner.

COVID-19 Vaccine: Between Myth and Truth.

Since December 2019, a pandemic caused by the newly identified SARS-CoV-2 spread across the entire globe, causing 364,191,494 confirmed cases of COVID-19 to date. SARS-CoV-2 is a betacoronavirus, a positive-sense, single-stranded RNA virus with four structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N). The S protein plays a crucial role both in cell binding and in the induction of a strong immune response during COVID-19 infection. The clinical impact of SARS-CoV-2 and its spread led to the urgent need for vaccine development to prevent viral transmission and to reduce the morbidity and mortality associated with the disease. Multiple platforms have been involved in the rapid development of vaccine candidates, with the S protein representing a major target because it can stimulate the immune system, yielding neutralizing antibodies (NAbs), blocking viral entry into host cells, and evoking T-cell immune responses. To date, 178 SARS-CoV-2 vaccine candidates have been challenged in clinical trials, of which 33 were approved by various national regulatory agencies. In this review, we discuss the FDA- and/or EMA-authorized vaccines that are mostly based on mRNA or viral vector platforms. Furthermore, we debunk false myths about the COVID-19 vaccine as well as discuss the impact of viral variants and the possible future developments.

Plant-air partition coefficients for thirteen urban conifer tree species: Estimating the best gas and particulate matter associated PAH removers.

Polycyclic Aromatic Hydrocarbons (PAHs) are an important class of pollutants which mostly come out from incomplete combustion of organic materials including fossil fuels. For this reason, they are often found at high concentrations in cities, contaminating air with their gas and particle phase. While European Union policies try to reduce their concentrations, huge efforts are still devoted to mitigate the pollution by PAHs. One such measure of mitigation is the use of plants in capturing PAHs and other chemicals due to the ability of leaves in filtering both gas and particle fractions. In this work thirteen conifer species among those frequently living in temperate and Mediterranean cities were selected and concentrations of 16 PAHs were measured in needles. The data for spruce were used to back-calculate air concentrations of PAHs which were later used to obtain specific equations of plant-air partition coefficient (KPA). The results showed that the difference in total PAH uptake between the most (Pinus pinaster) and the least (Picea abies) efficient tree was close to an order of magnitude. Looking to the capability of the different species in enriching the particulate matter (PM) associated fraction of PAHs on their needles, Pinus pinaster was instead the least important (3.4% of total PAHs), while Cupressus lusitanica was the most important (34% of total PAHs). The new KPA equations can be used to fine tune the PAH uptake of a specific amount of plant biomass on air concentration reduction.

Life cycle exposure of plants considerably affects root uptake of PCBs: Role of growth strategies and dissolved/particulate organic carbon variability.

Plant roots can accumulate organic chemicals, including PCBs, and this could be relevant in spreading chemicals through the food chain. To estimate such uptake, several equations are available in the literature, mostly developed in lab conditions, to obtain the root concentration factor (RCF). Here, a long-term (18 months) greenhouse experiment, using an aged, contaminated soil, was performed to reproduce root uptake in field-like conditions and to account for the ecological variability of exposure during the entire life cycle. Specific growth strategies (i.e., annual vs. perennial), root development (e.g., timing of root production and decaying), and soil parameters (e.g., dissolved organic carbon (DOC), and the particulate organic carbon (POC)) may interfere with the uptake of contaminants into the roots of plants. In this study, we investigate the effects of these factors on the RCF, obtained for 79 PCBs. New predictive equations were calculated for 5 different plants species at four different growth times (from few months to 1.5 years) and stages (growing vs maturity). The relationships highlighted a species-specific and time-dependent accumulation of PCB in plants roots, with higher RCFs in summer than in fall for some species, and the relevant influence of DOC and POC in affecting root uptake.