Carbon fiber-sampling combined flame ionization mass spectrometry for direct analysis of drugs in oral fluid.

Drug-impaired driving poses a significant risk of collisions and other hazardous accidents, emphasizing the urgent need for simple and rapid roadside detection methods. Oral fluid, as an easily collectible and non-invasive test material, has gained widespread use in detecting drug-impaired driving. In this study, we have devised a method for direct sampling using a carbon fiber bundle combined with flame ionization mass spectrometry. The essence of this method lies in the synergy between the adsorption properties of carbon fiber and the plasma characteristics of the flame. Leveraging the strong adsorption capabilities of the carbon fiber bundle allows for the use of a minimal sample size (<100 µL) during sampling, presenting a distinct advantage in the roadside inspection and sampling process. Throughout the flame ionization process, proteins and salts within the oral fluid matrix adhere well to the carbon fiber bundle, while small molecule targets can be efficiently desorbed and react with charged species in the flame, leading to ionization. The results demonstrate the successful development of carbon fiber-sampling combined flame ionization mass spectrometry, capable of qualitative and quantitative analysis of drugs in oral fluid without the need for sample pre-treatment. Its quantitative capabilities are sufficient for real sample detection, providing an effective analytical method for the roadside detection of drugs in oral fluids.

Quantitative Analysis of Three Synthetic Cannabinoids MDMB-4en-PINACA, ADB-BUTINACA, and ADB-4en-PINACA by Thermal-Assisted Carbon Fiber Ionization Mass Spectrometry.

Recently, synthetic cannabinoids (SCs) have emerged as new psychoactive substances (NPS) and have been frequently added to e-liquids, leading to their abuse. In order to detect SCs in e-liquids quickly and accurately, a thermal-assisted carbon fiber ionization mass spectrometry technique has been developed. The introduction of a heat source helps to reduce the matrix effects. The results indicate that the ratio of the slope of the matrix curve (e-liquids matrix) and the standard curve (methanol solution) for SCs analysis is close to 1, indicating a minimized matrix effect of this method. Furthermore, this method exhibits good quantitative ability when applied to real samples. It does not require sample pretreatment and is sensitive enough to directly quantify SCs in e-liquids. Our method is characterized by the ability to achieve rapid and direct quantitative analysis with minimized matrix effects. It provides a rapid and simple method for analyzing SCs in e-liquids.