Assuntos
Amiloidose/diagnóstico , Abscesso Encefálico/diagnóstico , Encéfalo/patologia , Paralisia Facial/etiologia , Rim/patologia , Mucormicose/diagnóstico , Insuficiência Renal/etiologia , Abuso de Substâncias por Via Intravenosa/complicações , Adulto , Amiloidose/complicações , Encéfalo/diagnóstico por imagem , Abscesso Encefálico/etiologia , Diagnóstico Diferencial , Disartria/etiologia , Glomerulonefrite/diagnóstico , Dependência de Heroína/complicações , Humanos , Rim/diagnóstico por imagem , Masculino , Mucormicose/complicações , Diálise Peritoneal , Insuficiência Renal/terapia , Tomografia Computadorizada por Raios X , UltrassonografiaAssuntos
Confusão/etiologia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Herpesvirus Humano 6/isolamento & purificação , Hospedeiro Imunocomprometido , Meningoencefalite/diagnóstico , Infecções por Roseolovirus/diagnóstico , Antivirais/uso terapêutico , Autopsia , Diagnóstico Diferencial , Evolução Fatal , Foscarnet/uso terapêutico , Reação Enxerto-Hospedeiro , Humanos , Leucemia Linfocítica Crônica de Células B/terapia , Masculino , Meningoencefalite/complicações , Meningoencefalite/virologia , Pessoa de Meia-Idade , Infecções por Roseolovirus/complicações , Infecções por Roseolovirus/tratamento farmacológico , Transplante HomólogoAssuntos
Colesteatoma/patologia , Otite Média/complicações , Osso Temporal/patologia , Colesteatoma/diagnóstico por imagem , Colesteatoma/etiologia , Doença Crônica , Diagnóstico Diferencial , Dor de Orelha/etiologia , Febre/etiologia , Cefaleia/etiologia , Perda Auditiva Condutiva/etiologia , Humanos , Imageamento por Ressonância Magnética , Masculino , Otite Média/diagnóstico , Recidiva , Tomografia Computadorizada por Raios X , Adulto JovemRESUMO
Accumulation of inflammatory microglia in Alzheimer's senile plaques is a hallmark of the innate response to beta-amyloid fibrils and can initiate and propagate neurodegeneration characteristic of Alzheimer's disease (AD). The molecular mechanism whereby fibrillar beta-amyloid activates the inflammatory response has not been elucidated. CD36, a class B scavenger receptor, is expressed on microglia in normal and AD brains and binds to beta-amyloid fibrils in vitro. We report here that microglia and macrophages, isolated from CD36 null mice, had marked reductions in fibrillar beta-amyloid-induced secretion of cytokines, chemokines, and reactive oxygen species. Intraperitoneal and stereotaxic intracerebral injection of fibrillar beta-amyloid in CD36 null mice induced significantly less macrophage and microglial recruitment into the peritoneum and brain, respectively, than in wild-type mice. Our data reveal that CD36, a major pattern recognition receptor, mediates microglial and macrophage response to beta-amyloid, and imply that CD36 plays a key role in the proinflammatory events associated with AD.
Assuntos
Doença de Alzheimer/imunologia , Peptídeos beta-Amiloides/imunologia , Antígenos CD36/fisiologia , Macrófagos Peritoneais/imunologia , Microglia/imunologia , Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/metabolismo , Animais , Antígenos CD36/genética , Antígenos CD36/imunologia , Adesão Celular/fisiologia , Células Cultivadas , Quimiocina CCL2/genética , Quimiocina CCL2/metabolismo , Quimiocinas/genética , Quimiocinas/imunologia , Quimiocinas/metabolismo , Quimiotaxia/fisiologia , Humanos , Imuno-Histoquímica , Interleucina-1/genética , Interleucina-1/imunologia , Interleucina-1/metabolismo , Macrófagos Peritoneais/citologia , Macrófagos Peritoneais/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microglia/citologia , Microglia/metabolismo , Placa Amiloide/imunologia , Placa Amiloide/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Candida albicans is an important cause of morbidity in hospitalized and immunosuppressed patients. Virulence factors of C. albicans include: filamentation, proteinases, adherence proteins and biofilm formation. The objective of this work was to use Galleria mellonella as a model to study the roles of C. albicans filamentation in virulence. We focused our study to five genes BCR1, FLO8, KEM1, SUV3 and TEC1 that have been shown to play a role in filamentation. Filaments are necessary for biofilm formation and evading interaction with macrophages in mammalian infections. Among the five mutant strain tested, we found that only the flo8/flo8 mutant strain did not form filaments within G. mellonella. This strain also exhibited reduced virulence in the larvae. Another strain that exhibited reduced pathogenicity in the G. mellonella model was tec1/tec1 but by contrast, the tec1/tec1 strain retained the ability to form filaments. Overexpression of TEC1 in the flo8/flo8 mutant restored filamentation but did not restore virulence in the larvae as well as in a mouse model of C. albicans infection. The filamentation phenotype did not affect the ability of hemocytes, the immune cells of G. mellonella, to associate with the various mutant strains of C. albicans. The capacities of the tec1/tec1 mutant and the flo8/flo8 TDH3-TEC1 strains to form filaments with impaired virulence suggest that filamentation alone is not sufficient to kill G. mellonella and suggest other virulence factors may be associated with genes that regulate filamentation.
Assuntos
Candida albicans/patogenicidade , Mariposas/microbiologia , Animais , Candida albicans/genética , Candida albicans/fisiologia , Candidíase/microbiologia , Modelos Animais de Doenças , Feminino , Histocitoquímica , Hifas/genética , Hifas/patogenicidade , Estimativa de Kaplan-Meier , CamundongosRESUMO
Evaluation of Cryptococcus neoformans virulence in a number of nonmammalian hosts suggests that C. neoformans is a nonspecific pathogen. We used the killing of Galleria mellonella (the greater wax moth) caterpillar by C. neoformans to develop an invertebrate host model system that can be used to study cryptococcal virulence, host immune responses to infection, and the effects of antifungal compounds. All varieties of C. neoformans killed G. mellonella. After injection into the insect hemocoel, C. neoformans proliferated and, despite successful phagocytosis by host hemocytes, killed caterpillars both at 37 degrees C and 30 degrees C. The rate and extent of killing depended on the cryptococcal strain and the number of fungal cells injected. The sequenced C. neoformans clinical strain H99 was the most virulent of the strains tested and killed caterpillars with inocula as low as 20 CFU/caterpillar. Several C. neoformans genes previously shown to be involved in mammalian virulence (CAP59, GPA1, RAS1, and PKA1) also played a role in G. mellonella killing. Combination antifungal therapy (amphotericin B plus flucytosine) administered before or after inoculation was more effective than monotherapy in prolonging survival and in decreasing the tissue burden of cryptococci in the hemocoel. The G. mellonella-C. neoformans pathogenicity model may be a substitute for mammalian models of infection with C. neoformans and may facilitate the in vivo study of fungal virulence and efficacy of antifungal therapies.
Assuntos
Cryptococcus neoformans/patogenicidade , Mariposas/microbiologia , Animais , Antifúngicos/farmacologia , Cryptococcus neoformans/efeitos dos fármacos , Cryptococcus neoformans/genética , Hemócitos/fisiologia , VirulênciaRESUMO
A pathological hallmark of Alzheimer's disease is the senile plaque, composed of beta-amyloid fibrils, microglia, astrocytes, and dystrophic neurites. We reported previously that class A scavenger receptors mediate adhesion of microglia and macrophages to beta-amyloid fibrils and oxidized low-density lipoprotein (oxLDL)-coated surfaces. We also showed that CD36, a class B scavenger receptor and an oxLDL receptor, promotes H(2)O(2) secretion by macrophages adherent to oxLDL-coated surfaces. Whether CD36 is expressed on microglia, and whether it plays a role in secretion of H(2)O(2) by microglia interacting with fibrillar beta-amyloid is not known. Using fluorescence-activated cell sorting analysis and immunohistochemistry, we found that CD36 is expressed on human fetal microglia, and N9-immortalized mouse microglia. We also found that CD36 is expressed on microglia and on vascular endothelial cells in the brains of Alzheimer's disease patients. Bowes human melanoma cells, which normally do not express CD36, gained the ability to specifically bind to surfaces coated with fibrillar beta-amyloid when transfected with a cDNA encoding human CD36, suggesting that CD36 is a receptor for fibrillar beta-amyloid. Furthermore, two different monoclonal antibodies to CD36 inhibited H(2)O(2) production by N9 microglia and human macrophages adherent to fibrillar beta-amyloid by approximately 50%. Our data identify a role for CD36 in fibrillar beta-amyloid-induced H(2)O(2) production by microglia, and imply that CD36 can mediate binding to fibrillar beta-amyloid. We propose that similar to their role in the interaction of macrophages with oxLDL, class A scavenger receptors and CD36 play complimentary roles in the interactions of microglia with fibrillar beta-amyloid.
Assuntos
Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/fisiologia , Encéfalo/metabolismo , Antígenos CD36/fisiologia , Proteínas de Membrana , Microglia/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptores Imunológicos/fisiologia , Receptores de Lipoproteínas , Peptídeos beta-Amiloides/química , Peptídeos beta-Amiloides/farmacologia , Animais , Anticorpos/farmacologia , Adesão Celular , Linhagem Celular , Linhagem Celular Transformada , Feto/citologia , Humanos , Macrófagos/fisiologia , Camundongos , Microglia/efeitos dos fármacos , Fragmentos de Peptídeos/metabolismo , Fragmentos de Peptídeos/farmacologia , Peróxidos/metabolismo , Fagócitos/efeitos dos fármacos , Fagócitos/metabolismo , Ratos , Receptores Depuradores , Receptores Depuradores Classe A , Receptores Depuradores Classe BRESUMO
The self-association of proteins to form amyloid fibrils has been implicated in the pathogenesis of a number of diseases including Alzheimer's, Parkinson's, and Creutzfeldt-Jakob diseases. We recently reported that the myeloid scavenger receptor CD36 initiates a signaling cascade upon binding to fibrillar beta-amyloid that stimulates recruitment of microglia in the brain and production of inflammatory mediators. This receptor plays a key role in the pathogenesis of atherosclerosis, prompting us to evaluate whether fibrillar proteins were present in atherosclerotic lesions that could initiate signaling via CD36. We show that apolipoprotein C-II, a component of very low and high density lipoproteins, readily forms amyloid fibrils that initiate macrophage inflammatory responses including reactive oxygen production and tumor necrosis factor alpha expression. Using macrophages derived from wild type and Cd36(-/-) mice to distinguish CD36-specific events, we show that fibrillar apolipoprotein C-II activates a signaling cascade downstream of this receptor that includes Lyn and p44/42 MAPKs. Interruption of this signaling pathway through targeted deletion of Cd36 or blocking of p44/42 MAPK activation inhibits macrophage tumor necrosis factor alpha gene expression. Finally, we demonstrate that apolipoprotein C-II in human atheroma co-localizes to regions positive for markers of amyloid and macrophage accumulation. Together, these data characterize a CD36-dependent signaling cascade initiated by fibrillar amyloid species that may promote atherogenesis.